IGF-I/IGF-1 Products

291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND₅₀ is typically 3-12 µg/mL." class="big_lightbox" target="_blank">

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

791-MG) was serially diluted and captured by Hamster Anti-Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">