IGF-I/IGF-1 Antibody [Unconjugated]

Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3‑12 µg/mL.

IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) . This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IGF-I/IGF-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IGF-I/IGF-1
• Specificity: Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGF1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 3-12 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I/IGF-1 Antibody [Unconjugated]

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-I/IGF-1 Antibody (AF-291-NA)(26)

Publications using AF-291-NA

• KM Lugo-Cintr, JM Ayuso, M Humayun, MM Gong, SC Kerr, SM Ponik, PM Harari, M Virumbrale, DJ Beebe Primary Head and Neck Tumour-Derived Fibroblasts Promote Lymphangiogenesis in a Lymphatic Organotypic Co-culture Model EBioMedicine, 2021;73(0):103634. 2021 [PMID: 34673450] (Neutralization, Human)
• Q Zhang, T Li, Z Wang, X Kuang, N Shao, Y Lin lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32548873] (Western Blot, Human)
• F Alvarez-Ri, A Rey-Rico, JK Venkatesan, L Diaz-Gomez, M Cucchiarin, A Concheiro, C Alvarez-Lo Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy Pharmaceutics, 2020;12(4):. 2020 [PMID: 32283694] (IHC, Human)
• S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis Microorganisms, 2019;7(10):. 2019 [PMID: 31569536] (Neutralization, Human)
• S Morscheid, A Rey-Rico, G Schmitt, H Madry, M Cucchiarin, JK Venkatesan Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 2019;20(10):. 2019 [PMID: 31137788] (IHC-P, Human)
• M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer Cancers (Basel), 2019;11(5):. 2019 [PMID: 31086083] (Neutralization, Human)
• A Pristerà, C Blomeley, E Lopes, S Threlfell, E Merlini, D Burdakov, S Cragg, F Guillemot, SL Ang Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration Proc. Natl. Acad. Sci. U.S.A., 2019;116(9):3817-3826. 2019 [PMID: 30808767] (IHC, Mouse)
• M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30609691] (Neutralization, Human)
• L Dou, Y Zheng, L Li, X Gui, Y Chen, M Yu, Y Guo The effect of cinnamon on polycystic ovary syndrome in a mouse model Reprod. Biol. Endocrinol., 2018;16(1):99. 2018 [PMID: 30340496] (IHC, Mouse)
• S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Neutralization, Human)
• D Wang, C Liu, J Wang, Y Jia, X Hu, H Jiang, ZM Shao, YA Zeng Protein C receptor stimulates multiple signaling pathways in breast cancer cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29217770] (Neutralization, Human)
• L Song, Z Sun, DS Kim, W Gou, C Strange, H Dong, W Cui, G Gilkeson, KA Morgan, DB Adams, H Wang Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function Stem Cell Res Ther, 2017;8(1):192. 2017 [PMID: 28854965] (Neutralization, Mouse)
• J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28467017] (ICC, Human)
• Magali Cucchiarin Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I Tissue Eng Part A, 2015;21(17):2460-71. 2015 [PMID: 26123891] (IHC-P, Human)
• Benabbou N, Mirshahi P, Bordu C, Faussat A, Tang R, Therwath A, Soria J, Marie J, Mirshahi M A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line. Int J Oncol, 2014;45(4):1372-80. 2014 [PMID: 25095896]
• Du , Lili, Yu , Yanqiu, Ma , Haiying, Lu , Xiaomei, Ma , Ling, Jin , Yunan, Zhang , Haipeng Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1. Int J Mol Sci, 2014;15(2):1983-2002. 2014 [PMID: 24473145] (Neutralization, Human)
• Ni , Fang, Sun , Rui, Fu , Binqing, Wang , Fuyan, Guo , Chuang, Tian , Zhigang, Wei , Haiming IGF-1 promotes the development and cytotoxic activity of human NK cells. Nat Commun, 2013;4(0):1479. 2013 [PMID: 23403580] (Neutralization, Human)
• Weimer A, Madry H, Venkatesan JK Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis. Mol. Med., 2012;18(1):346-58. 2012 [PMID: 22160392] (Western Blot, Human)
• Banziger-Tobler NE, Halin C, Kajiya K, Detmar M Growth hormone promotes lymphangiogenesis. Am. J. Pathol., 2008;173(2):586-97. 2008 [PMID: 18583315] (ELISA Development, Human)
• Skibinski G, Elborn JS, Ennis M Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76. 2007 [PMID: 17384084] (Neutralization, Human)
• Yoon DM, Hawkins EC, Francke-Carroll S, Fisher JP Effect of construct properties on encapsulated chondrocyte expression of insulin-like growth factor-1. Biomaterials, 2006;28(2):299-306. 2006 [PMID: 16982090] (Western Blot, Bovine)
• Schnapp LM, Donohoe S, Chen J, Sunde DA, Kelly PM, Ruzinski J, Martin T, Goodlett DR Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury. Am. J. Pathol., 2006;169(1):86-95. 2006 [PMID: 16816363] (Neutralization, Human)
• McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori. Gastroenterology, 2006;130(6):1754-63. 2006 [PMID: 16697739] (Western Blot, Human)
• Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2006;47(3):864-72. 2006 [PMID: 16505018] (Western Blot, Human)
• Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL Gene induction during differentiation of human pulmonary type II cells in vitro. Am. J. Respir. Cell Mol. Biol., 2006;34(6):727-37. 2006 [PMID: 16474099] (Western Blot, Human)
• Brenner N, Huning M, Stange R Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing. Calcif. Tissue Int., 2004;74(4):388-97. 2004 [PMID: 15255077] (IHC-P, Rat)

Reviews for IGF-I/IGF-1 Antibody (AF-291-NA) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous - WB Human 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24036101
• Species: Human

reviewed by: Anonymous - WB Human 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23424195
• Species: Human

Bioinformatics

• Gene Symbol: IGF1