IGF-I/IGF-1 Antibody [Unconjugated]

Images

 
IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 ...read more
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ...read more
IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

IGF-I/IGF-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Specificity
Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGF1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 3-12 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF-291-NA in the following applications:

Publications
Read Publications using
AF-291-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I/IGF-1 Antibody [Unconjugated]

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-I/IGF-1 Antibody (AF-291-NA)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 6 applications: ELISA Development, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC
(2)
IHC-P
(3)
Neutralization
(9)
Western Blot
(5)
All Applications
Filter By Species
Human
(16)
Mouse
(3)
Rat
(1)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF-291-NA Applications Species
S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis Microorganisms, 2019;7(10):. 2019 [PMID: 31569536] (Neutralization, Human) Neutralization Human
S Morscheid, A Rey-Rico, G Schmitt, H Madry, M Cucchiarin, JK Venkatesan Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 2019;20(10):. 2019 [PMID: 31137788] (IHC-P, Human) IHC-P Human
A Pristerà, C Blomeley, E Lopes, S Threlfell, E Merlini, D Burdakov, S Cragg, F Guillemot, SL Ang Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration Proc. Natl. Acad. Sci. U.S.A., 2019;116(9):3817-3826. 2019 [PMID: 30808767] (IHC, Mouse) IHC Mouse
M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30609691] (Neutralization, Human) Neutralization Human
L Dou, Y Zheng, L Li, X Gui, Y Chen, M Yu, Y Guo The effect of cinnamon on polycystic ovary syndrome in a mouse model Reprod. Biol. Endocrinol., 2018;16(1):99. 2018 [PMID: 30340496] (IHC, Mouse) IHC Mouse
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Neutralization, Human) Neutralization Human
D Wang, C Liu, J Wang, Y Jia, X Hu, H Jiang, ZM Shao, YA Zeng Protein C receptor stimulates multiple signaling pathways in breast cancer cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29217770] (Neutralization, Human) Neutralization Human
L Song, Z Sun, DS Kim, W Gou, C Strange, H Dong, W Cui, G Gilkeson, KA Morgan, DB Adams, H Wang Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function Stem Cell Res Ther, 2017;8(1):192. 2017 [PMID: 28854965] (Neutralization, Mouse) Neutralization Mouse
J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28467017] (ICC, Human) ICC Human
Magali Cucchiarin Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I Tissue Eng Part A, 2015;21(17):2460-71. 2015 [PMID: 26123891] (IHC-P, Human) IHC-P Human
Show All 22 Publications.

Reviews for IGF-I/IGF-1 Antibody (AF-291-NA) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF-291-NA:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24036101
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24036101

Details

Detection NotesSee PMID 24036101

Comments

CommentsPublished: See PMID 24036101
 
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23424195
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23424195

Details

Detection NotesSee PMID 23424195

Comments

Commentswe have used this antibody for western blot, ELISA and coimmunoprecipitation assays. we have not observed any non-specific bands in our assays. See PMID 23424195

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FAQs for IGF-I/IGF-1 Antibody (AF-291-NA) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF291

Additional IGF-I/IGF-1 Products

Bioinformatics Tool for IGF-I/IGF-1 Antibody (AF-291-NA)

Discover related pathways, diseases and genes to IGF-I/IGF-1 Antibody (AF-291-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IGF-I/IGF-1 Antibody (AF-291-NA)

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Pathways for IGF-I/IGF-1 Antibody (AF-291-NA)

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PTMs for IGF-I/IGF-1 Antibody (AF-291-NA)

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Recombinant Monoclonal Antibodies

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: WB
Species: Human

 
Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGF1
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