IGF-I/IGF-1 Antibody [Unconjugated]

Images

 
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ...read more
IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room ...read more
IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 ...read more
Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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IGF-I/IGF-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Specificity
Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGF1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 3-12 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF-291-NA in the following applications:

Publications
Read Publications using
AF-291-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I/IGF-1 Antibody [Unconjugated]

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-I/IGF-1 Antibody (AF-291-NA)(26)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 6 applications: ELISA Development, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC
(3)
IHC-P
(3)
Neutralization
(11)
Western Blot
(6)
All Applications
Filter By Species
Human
(20)
Mouse
(3)
Rat
(1)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF-291-NA Applications Species
KM Lugo-Cintr, JM Ayuso, M Humayun, MM Gong, SC Kerr, SM Ponik, PM Harari, M Virumbrale, DJ Beebe Primary Head and Neck Tumour-Derived Fibroblasts Promote Lymphangiogenesis in a Lymphatic Organotypic Co-culture Model EBioMedicine, 2021;73(0):103634. 2021 [PMID: 34673450] (Neutralization, Human) Neutralization Human
Q Zhang, T Li, Z Wang, X Kuang, N Shao, Y Lin lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32548873] (Western Blot, Human) Western Blot Human
F Alvarez-Ri, A Rey-Rico, JK Venkatesan, L Diaz-Gomez, M Cucchiarin, A Concheiro, C Alvarez-Lo Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy Pharmaceutics, 2020;12(4):. 2020 [PMID: 32283694] (IHC, Human) IHC Human
S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis Microorganisms, 2019;7(10):. 2019 [PMID: 31569536] (Neutralization, Human) Neutralization Human
S Morscheid, A Rey-Rico, G Schmitt, H Madry, M Cucchiarin, JK Venkatesan Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 2019;20(10):. 2019 [PMID: 31137788] (IHC-P, Human) IHC-P Human
M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer Cancers (Basel), 2019;11(5):. 2019 [PMID: 31086083] (Neutralization, Human) Neutralization Human
A Pristerà, C Blomeley, E Lopes, S Threlfell, E Merlini, D Burdakov, S Cragg, F Guillemot, SL Ang Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration Proc. Natl. Acad. Sci. U.S.A., 2019;116(9):3817-3826. 2019 [PMID: 30808767] (IHC, Mouse) IHC Mouse
M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30609691] (Neutralization, Human) Neutralization Human
L Dou, Y Zheng, L Li, X Gui, Y Chen, M Yu, Y Guo The effect of cinnamon on polycystic ovary syndrome in a mouse model Reprod. Biol. Endocrinol., 2018;16(1):99. 2018 [PMID: 30340496] (IHC, Mouse) IHC Mouse
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Neutralization, Human) Neutralization Human
Show All 26 Publications.

Reviews for IGF-I/IGF-1 Antibody (AF-291-NA) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF-291-NA:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24036101
SpeciesHuman
  4
reviewed by:
Anonymous -
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23424195
SpeciesHuman

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FAQs for IGF-I/IGF-1 Antibody (AF-291-NA) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for IGF-I/IGF-1 Antibody (AF-291-NA)

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Recent Reviews

4.5
5
1
4
1
3
0
2
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1
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Anonymous -
01/07/2015
Application: WB
Species: Human

 
Anonymous -
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGF1