IGF-I/IGF-1 Antibody [Biotin]

Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF291) . This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IGF-I/IGF-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human IGF-I/IGF-1
• Specificity: Detects human IGF-I/IGF-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% recombinant human (rh) IGF-I/IGF-1 sR, recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, rhIGF-II/IGF-2, rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human IGF-I Antibody (Catalog # MAB291)
  ELISA Detection: Human IGF-I Biotinylated Antibody (Catalog # BAF291)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I/IGF-1 Antibody [Biotin]

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4 - 7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

1. Philippou, A. et al. (2007) In Vivo 21:45.
2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-I/IGF-1 Antibody (BAF291)(7)

Publications using BAF291

• J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs Adv Healthc Mater, 2018-02-14;0(0):. 2018-02-14 [PMID: 29441702] (ELISA Detection, Mouse)
• Ludwig S, Tokarski C, Lang S, van Ginkel L, Zhu H, Ozcan A, Nielen M Calling Biomarkers in Milk Using a Protein Microarray on Your Smartphone. PLoS ONE, 2015-08-26;10(8):e0134360. 2015-08-26 [PMID: 26308444] (Array Development, Bovine)
• Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence. PLoS ONE, 2014-06-17;9(6):e99983. 2014-06-17 [PMID: 24937130] (ELISA Development (Detection), Human)
• E M Goetz, B Shankar, Y Zou, J C Morales, X Luo, S Araki et al. ATM-dependent IGF-1 induction regulates secretory clusterin expression after DNA damage and in genetic instability Oncogene 9/1/2011 [PMID: 21460853]
• E M Goetz, B Shankar, Y Zou, J C Morales, X Luo, S Araki et al. ATM-dependent IGF-1 induction regulates secretory clusterin expression after DNA damage and in genetic instability Oncogene 2011-09-01 [PMID: 21460853] (ELISA Detection, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy. Clin. Cancer Res., 2007-02-01;13(3):1000-9. 2007-02-01 [PMID: 17289896] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IGF1