IFN-gamma Biologically Active Protein



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IFN-gamma Biologically Active Protein Summary

A biologically active recombinant protein with a 16 residue His tag and corresponding to 159 total residues of IFNG.

Source:E. coli The recombinant protein migrates as two bands with an apparent molecular mass of 25 and 27 kDa in SDS-PAGE.
Produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Details of Functionality
The bioactivity of recombinant human IFN-gamma was determined by its dose dependent cytostatic effect, in the presence of TNF-alpha, on HT-29 (human carcinoma) cells. The ED50 value for this batch of IFN-g is 0.12 ng/ml, corresponding to 8.5 x 10e6 U/mg specific activity.
Endotoxin Note
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kinetic assay.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Sterile filtered through a 0.2 um filter.PBS, pH 7.2.
No Preservative
Reconstitution Instructions
Reconstitute with deionized water to final concentration of at least 0.1 mg/ml.


Application Notes
This protein is functionally active and can be added to cell culture without the need to remove any Bioactive molecules. NovActive recombinant human IFN-gamma is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.

Reactivity Notes

Human Recombinant protein


Greater than 95% by SDS-PAGE gel analysis.


IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Gene Symbol IFNG

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