IFN-gamma Antibody [Unconjugated]

Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA). The ND50 is typically0.2-1.0 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IFN-gamma Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IFN-gamma
• Specificity: Detects mouse IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human IFN‑ gamma , recombinant cotton rat IFN‑ gamma , recombinant feline IFN‑ gamma , recombinant rat IFN‑ gamma , recombinant porcine IFN‑ gamma , and recombinant canine IFN‑ gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNG
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.2-1.0 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody [Unconjugated]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (AF-485-NA)(5)

Publications using AF-485-NA

• H Wang, X Feng, P Han, Y Lei, Y Xia, D Tian, W Yan The JAK inhibitor tofacitinib ameliorates immune?mediated liver injury in mice Mol Med Rep, 2019-10-16;0(0):. 2019-10-16 [PMID: 31638166] (Western Blot, Mouse)
• Y Manome, D Suzuki, A Mochizuki, E Saito, K Sasa, K Yoshimura, T Inoue, M Takami, K Inagaki, T Funatsu, R Kamijo The inhibition of malignant melanoma cell invasion of bone by the TLR7 agonist R848 is dependent upon pro-inflammatory cytokines produced by bone marrow macrophages Oncotarget, 2018-07-06;9(52):29934-29943. 7/6/2018 [PMID: 30042824]
• Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. J. Clin. Invest., 2011-09-19;121(10):3846-59. 2011-09-19 [PMID: 21926464] (Neutralization, Mouse)
• Tilahun AY, Holz M, Wu TT, David CS, Rajagopalan G Interferon gamma-dependent intestinal pathology contributes to the lethality in bacterial superantigen-induced toxic shock syndrome. PLoS ONE, 2011-02-03;6(2):e16764. 2011-02-03 [PMID: 21304813] (Neutralization, Mouse)
• Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9. 2009-03-16 [PMID: 19289819] (Neutralization, Mouse)

FAQs for IFN-gamma Antibody (AF-485-NA). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG