IFN-gamma Antibody (H22) [Unconjugated]

IFN‑ gamma was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA (left panel) or unstimulated (right panel) using Hamster Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB4851) at 15 µg/mL for 3 hours at room temperature. Cells were stained using an anti-hamster IgG secondary antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Mouse IFN‑ gamma (Catalog # 485‑MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti‑Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB4851). The ND50 is typically 4‑20 ng/mL.

• Reactivity: Mu
• Applications: B/N, ICC/IF
• Clone: H22
• Clonality: Monoclonal
• Host: Hamster
• Conjugate: Unconjugated

IFN-gamma Antibody (H22) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IFN-gamma
  Accession # P01580
• Specificity: Detects mouse IFN-gamma in direct ELISAs. Does not cross-react with rat, cotton rat, human, rhesus macaque, porcine, feline, or canine IFN-gamma.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Hamster
• Gene: IFNG
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 4-20 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody (H22) [Unconjugated]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (MAB4851)(8)

Publications using MAB4851

• RC Davicino, SP Méndez-Hue, RJ Eliçabe, JC Stupirski, I Autenrieth, MS Di Genaro, GA Rabinovich Galectin-1-Driven Tolerogenic Programs Aggravate Yersinia enterocolitica Infection by Repressing Antibacterial Immunity J. Immunol., 2017;0(0):. 2017 [PMID: 28716827] (In Vivo, Mouse)
• El-Behi M, Ciric B, Dai H The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF. Nat. Immunol., 2011;12(6):568-75. 2011 [PMID: 21516111] (Neutralization, Mouse)
• Elicabe RJ, Cargnelutti E, Serer MI, Stege PW, Valdez SR, Toscano MA, Rabinovich GA, Di Genaro MS Lack of TNFR p55 results in heightened expression of IFN-gamma and IL-17 during the development of reactive arthritis. J. Immunol., 2010;185(7):4485-95. 2010 [PMID: 20810989] (Neutralization, Mouse)
• Srivastava K, Zhang T, Yang N, Sampson H, Li XM Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-gamma, but not transforming growth factor-beta dependent. Clin. Exp. Allergy, 2010;40(11):1678-88. 2010 [PMID: 20573156] (Neutralization, Mouse)
• Wildbaum G, Zohar Y, Karin N Antigen-specific CD25- Foxp3- IFN-gamma(high) CD4+ T cells restrain the development of experimental allergic encephalomyelitis by suppressing Th17. Am. J. Pathol., 2010;176(6):2764-75. 2010 [PMID: 20382706] (Neutralization, Mouse)
• Ito H, Ando K, Ishikawa T Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance. Int. Immunol., 2008;20(7):869-79. 2008 [PMID: 18487227] (Neutralization, Mouse)
• Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection. J. Immunol., 2008;180(1):113-21. 2008 [PMID: 18097010] (Neutralization, Mouse)
• Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation. J. Clin. Invest., 2007;117(12):3868-3878. 2007 [PMID: 18060034] (Neutralization, Mouse)

FAQs for IFN-gamma Antibody (MAB4851). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • P01580()