ELISA: Purified anti-human IFN-g antibody (clone 43-11) can be used as the capture reagent in a two-site sandwich ELISA for measuring human IFN-g levels in conjunction with the biotinylated form of the anti-human IFN-g monoclonal (clone 45-15 Cat. No. IMG-211B) as the detecting antibody (1), and recombinant human IFN-g as the standard. The optimal concentration of clone 43-11 for coating ELISA plates was 5 mg/ml. This ELISA measured human IFN-g with a detection limit of 37 pg/ml (1). The detection limit can be increased to 15 pg/ml with the use of a bead-ELISA assay (1). Chemiluminescent Immuno Assay (CLIA): Clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used together as an antibody pair to implement a highly sensitive Chemiluminescent Immuno Assay (CLIA) for human IFN-g (0.5 pg/ml as the detection limit) and a bead-based CLIA (detection limit of 0.2 pg/ml) (1). Cytokine Immunotrapping Assay (CITA): Furthermore clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used in combination to study the early production of human IFN-g in a highly sensitive chemiluminescent based Cytokine Immuno-trapping Assay (CITA) (2). Neutralization: This antibody is not suitable for neutralization studies. For neutralization of the biologic activity of human IFN-g the use of clone 45-15 (Cat. No. IMG-211E) is recommended (1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
iNOS: The Nitric Oxide Boss Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti... Read full blog post.