IFN-gamma Antibody (43-11)

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Western Blot: IFN-gamma Antibody (43-11) [NBP2-31044] - analysis of IFN-gamma in HEK293 cell lysate using anti-IFN-gamma antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, CITA, CLIA, ELISA
Clone
43-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

IFN-gamma Antibody (43-11) Summary

Immunogen
Monoclonal Antibody to Human IFN-g (ELISA Capture)-Azide Free
Specificity
This antibody recognizes both the recombinant and the natural form of human interferon-gamma (IFN-gamma) (1). This antibody does not react with human IL-4, IFN-alpha or acid inactivated IFN-gamma, and it shows only a minimal reactivity with heatinactivated IFN-g (<0.05%) (1).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IFNG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Blocking/Neutralizing
  • ELISA >15 pg/ml
  • Chemiluminescence Immunoassay >0.2 pg/ml
  • Cytokine immunotrapping Assay
Reviewed Applications
Read 1 Review rated 5
using
NBP2-31044 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 8.0)
Preservative
0.1% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IFN-gamma Antibody (43-11)

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

ELISA: Purified anti-human IFN-g antibody (clone 43-11) can be used as the capture reagent in a two-site sandwich ELISA for measuring human IFN-g levels in conjunction with the biotinylated form of the anti-human IFN-g monoclonal (clone 45-15 Cat. No. IMG-211B) as the detecting antibody (1), and recombinant human IFN-g as the standard. The optimal concentration of clone 43-11 for coating ELISA plates was 5 mg/ml. This ELISA measured human IFN-g with a detection limit of 37 pg/ml (1). The detection limit can be increased to 15 pg/ml with the use of a bead-ELISA assay (1). Chemiluminescent Immuno Assay (CLIA): Clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used together as an antibody pair to implement a highly sensitive Chemiluminescent Immuno Assay (CLIA) for human IFN-g (0.5 pg/ml as the detection limit) and a bead-based CLIA (detection limit of 0.2 pg/ml) (1). Cytokine Immunotrapping Assay (CITA): Furthermore clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used in combination to study the early production of human IFN-g in a highly sensitive chemiluminescent based Cytokine Immuno-trapping Assay (CITA) (2). Neutralization: This antibody is not suitable for neutralization studies. For neutralization of the biologic activity of human IFN-g the use of clone 45-15 (Cat. No. IMG-211E) is recommended (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IFN-gamma Antibody (NBP2-31044) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-31044:
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Images Ratings Applications Species Date Details
Western Blot IFN-gamma NBP2-31044
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reviewed by:
Shilpa Agarwal
WB Human 11/26/2014
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Summary

ApplicationWestern Blot
Sample TestedLysate from HEK293 cells
SpeciesHuman

Blocking

Blocking DetailsNon-fat dry milk (5%) in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 dilution, 3 hrs at RT in 5% non-fat dry milk

Secondary Antibody

Secondary Descriptionanti mouse HRP-conjugated antibody
Secondary Manufacturer Cat#Life Technologies # A16066
Secondary Concentration1:5,000

Details

Detection NotesSuperSignal West Pico Chemiluminescent Substrate (Thermo), 30 sec exposure using X-ray film

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Shilpa Agarwal
11/26/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol IFNG
Entrez
Uniprot