IFN-gamma Antibody (43-11) - BSA Free

Western Blot: IFN-gamma Antibody (43-11) [NBP2-31044] - analysis of IFN-gamma in HEK293 cell lysate using anti-IFN-gamma antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, Func, B/N
• Clone: 43-11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

IFN-gamma Antibody (43-11) - BSA Free Summary

• Description: Novus Biologicals Mouse IFN-gamma Antibody (43-11) - BSA Free (NBP2-31044) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Monoclonal Antibody to Human IFN-g (ELISA Capture)-Azide Free
• Specificity: This antibody recognizes both the recombinant and the natural form of human interferon-gamma (IFN-gamma) (1). This antibody does not react with human IL-4, IFN-alpha or acid inactivated IFN-gamma , and it shows only a minimal reactivity with heatinactivated IFN-g (<0.05%) (1).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Chemiluminescence Immunoassay >0.2 pg/ml
  • Cytokine immunotrapping Assay
  • ELISA >15 pg/ml
  • Western Blot
• Application Notes: WB reported in a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 8.0)
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for IFN-gamma Antibody (43-11) - BSA Free

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

ELISA: Purified anti-human IFN-g antibody (clone 43-11) can be used as the capture reagent in a two-site sandwich ELISA for measuring human IFN-g levels in conjunction with the biotinylated form of the anti-human IFN-g monoclonal (clone 45-15 Cat. No. IMG-211B) as the detecting antibody (1), and recombinant human IFN-g as the standard. The optimal concentration of clone 43-11 for coating ELISA plates was 5 mg/ml. This ELISA measured human IFN-g with a detection limit of 37 pg/ml (1). The detection limit can be increased to 15 pg/ml with the use of a bead-ELISA assay (1). Chemiluminescent Immuno Assay (CLIA): Clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used together as an antibody pair to implement a highly sensitive Chemiluminescent Immuno Assay (CLIA) for human IFN-g (0.5 pg/ml as the detection limit) and a bead-based CLIA (detection limit of 0.2 pg/ml) (1). Cytokine Immunotrapping Assay (CITA): Furthermore clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used in combination to study the early production of human IFN-g in a highly sensitive chemiluminescent based Cytokine Immuno-trapping Assay (CITA) (2). Neutralization: This antibody is not suitable for neutralization studies. For neutralization of the biologic activity of human IFN-g the use of clone 45-15 (Cat. No. IMG-211E) is recommended (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IFN-gamma Antibody (NBP2-31044) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Shilpa Agarwal WB Human 11/26/2014

Summary

• Application: Western Blot
• Sample Tested: Lysate from HEK293 cells
• Species: Human

FAQs for IFN-gamma Antibody (NBP2-31044). (Showing 1 - 1 of 1 FAQ).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • NP_004855(Human)