IDO2 Antibody (OTI1A4)

Western Blot: IDO2 Antibody (1A4) [NBP2-02730] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2.

Flow Cytometry: IDO2 Antibody (1A4) [NBP2-02730] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IDO2 antibody, and then analyzed by flow cytometry.

Flow Cytometry: IDO2 Antibody (1A4) [NBP2-02730] - Analysis of Jurkat cells, using anti-IDO2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow
• Clone: OTI1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IDO2 Antibody (OTI1A4) Summary

• Immunogen: Full length human recombinant protein of human IDO2 (NP_919270) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Western Blot 1:500
• Theoretical MW: 46.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IDO2 Antibody (OTI1A4)

• EC 1.13.11.-
• IDO2
• Ido-2
• INDOL1
• INDOL1indoleamine-pyrrole 2,3 dioxygenase-like 1
• indoleamine 2,3-dioxygenase 2
• Indoleamine 2,3-dioxygenase-2
• indoleamine 2,3-dioxygenase-like 1 protein
• Indoleamine 2,3-dioxygenase-like protein 1
• Indoleamine-pyrrole 2,3-dioxygenase-like protein 1

Background

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IDO2 Antibody (NBP2-02730). (Showing 1 - 1 of 1 FAQ).

1. I have a question regarding the use of the IDO2 antibody (NBP2-02730) for flow. Do you have any protocols or special recommendations for using this antibody for flow, since IDO2 is an intracellular enzyme?
   • Although our general protocol posted on our website is not very specific, it could be referenced as the first step. Here is another protocol from R&D Systems (our sister company) addressing the steps of intracellular staining in flow

Bioinformatics

• Gene Symbol: IDO2
• Entrez:
  • 169355(Human)