ICOS Antibody (ISA-3)


Flow Cytometry: ICOS Antibody (ISA-3) [NBP1-43126] - Staining of CD3 + CD28 stimulated human PBMC with Anti-Human CD278 (ICOS) FITC (left), and Anti-Human CD278 (ICOS) PE (right). Appropriate isotype controls were used ...read more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, Func, IHC, IHC-Fr, IP
0.5 mg/ml

ICOS Antibody (ISA-3) Summary

The immunogen for this antibody was ICOS.
IgG1 Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The ISA-3 antibody has been tested by flow cytometric analysis of unstimulated and PHA-activated (2 days) human blood leukocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Alternate Names for ICOS Antibody (ISA-3)

  • Activation-inducible lymphocyte immunomediatory molecule
  • AILIMCD278
  • CD278 antigen
  • CD278
  • CRP-1
  • CVID1
  • inducible costimulator
  • inducible T-cell costimulator
  • inducible T-cell co-stimulator
  • MGC39850


The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4, was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3 however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use or rather than.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP1-43472
FITC Labeled NBP1-43917
PE Labeled NBP1-43707
PE/Cy7 Labeled NBP1-42933

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Blogs on ICOS

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Contact Information

Product PDFs


Gene Symbol ICOS

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