ICAP-1 Overexpression Lysate (Native)

Western Blot: ICAP1 Overexpression Lysate (Adult Normal) [NBL1-12071] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAP1.

• Reactivity: Hu
• Applications: WB

ICAP-1 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC201963 and based on accession number NM_004763. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens ICAP1, mRNA.
• Gene: ITGB1BP1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 21.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ICAP-1 Overexpression Lysate (Native)

• Bodenin
• DKFZp686K08158
• ICAP1
• ICAP-1
• ICAP-1alpha
• ICAP-1B
• ICAP1ICAP1AICAP1BICAP-1A
• integrin beta 1 binding protein 1
• integrin beta-1-binding protein 1
• Integrin cytoplasmic domain-associated protein 1
• integrin cytoplasmic domain-associated protein 1-alpha
• integrin cytoplasmic domain-associated protein 1-beta
• ITGB1BP1

Background

The cytoplasmic domains of integrins are essential for cell adhesion. The protein encoded by this gene binds to the beta1 integrin cytoplasmic domain. The interaction between this protein and beta1 integrin is highly specific. Two isoforms of this protein are derived from alternatively spliced transcripts. The shorter form of this protein does not interact with the beta1 integrin cytoplasmic domain. The longer form is a phosphoprotein and the extent of its phosphorylation is regulated by the cell-matrix interaction, suggesting an important role of this protein during integrin-dependent cell adhesion. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the full length isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ITGB1BP1
• Entrez:
  • 9270(Human)
  • 16413(Mouse)
  • 298914(Rat)