ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free

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Western Blot: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM-3.
Immunocytochemistry/ Immunofluorescence: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ICAM3.
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of extracts (35ug) from 9 different cell lines by using ICAM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI1E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ICAM3(NP_002153) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICAM3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400-4000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
Theoretical MW
56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ICAM-3/CD50 Antibody (NBP2-70975) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70975AF350
Alexa Fluor 405 NBP2-70975AF405
Alexa Fluor 488 NBP2-70975AF488
Alexa Fluor 532 NBP2-70975AF532
Alexa Fluor 594 NBP2-70975AF594
Alexa Fluor 647 NBP2-70975AF647
Alexa Fluor 700 NBP2-70975AF700
Alexa Fluor 750 NBP2-70975AF750
Biotin NBP2-70975B
DyLight 350 NBP2-70975UV
DyLight 405 NBP2-70975V
DyLight 488 NBP2-70975G
DyLight 550 NBP2-70975R
DyLight 594 NBP2-70975DL594
DyLight 650 NBP2-70975C
DyLight 680 NBP2-70975FR
DyLight 755 NBP2-70975IR
FITC NBP2-70975F
HRP NBP2-70975H
Janelia Fluor 549 NBP2-70975JF549
Janelia Fluor 646 NBP2-70975JF646

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Bioinformatics Tool for ICAM-3/CD50 Antibody (NBP2-70975)

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Blogs on ICAM-3/CD50

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Bioinformatics

Gene Symbol ICAM3