ICAM-3/CD50 Antibodies

715-IC) was serially diluted and captured by Mouse Anti-Human ICAM‑3/CD50 Monoclonal Antibody (Catalog # BBA15) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM‑3/CD50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF813) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 715-IC) was serially diluted and captured by Mouse Anti-Human ICAM‑3/CD50 Monoclonal Antibody (Catalog # BBA15) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM‑3/CD50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF813) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC) was serially diluted and captured by Mouse Anti-Human ICAM‑3/CD50 Monoclonal Antibody (Catalog # BBA15) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM‑3/CD50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF813) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB002), followed by Allophycocyanin-conjugated anti-Mouse IgG secondary antibody (F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB002), followed by Allophycocyanin-conjugated anti-Mouse IgG secondary antibody (F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # MAB8131) or (B) Mouse IgG1 Isotype Control (MAB002), followed by Allophycocyanin-conjugated anti-Mouse IgG secondary antibody (F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />