Human ICAM-1/CD54 ELISA Kit (Colorimetric)

ELISA: ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-82420] - Representative standard curve for human ICAM-1 ELISA. Human ICAM-1 was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be runfor each group of microwell strips assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, heparin, citrate), amniotic fluid, spontaneous urine, bile, other body fluids
• Standard Curve Range: 6.25 - 100 ng/mL
• Sensitivity: 2.2 ng/mL

Human ICAM-1/CD54 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: This antibody is reactive to the soluble protein. There was no cross reactivity detected with TNF-R (60 kDa), TNF-R (80 kDa), IL-8/NAP-1, TNF alpha, TNF beta, IFN gamma, IFN alpha 2C, IFN omega, IL-6, IL-2R, ELAM-1 and L-selectin.
• Standard Curve Range: 6.25 - 100 ng/mL
• Sensitivity: 2.2 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.7%
• Intra-Assay: CV% < 4.1%
• Spike Recovery: The recovery ranged from 82% to 109% with an overall mean recovery of 99%.
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ICAM1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ICAM-1/CD54 ELISA Kit (Colorimetric)

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR. sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ICAM1