ICAM-1/CD54 Antibody (MEM-111)

Images

 
Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Of CD54 expression in activated (A) and nonactivated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control.
Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using PE conjugate of NB500-318. A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched ...read more
Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using the FITC conjugate of NB500-318. Staining of Human peripheral blood lymphocytes with MEM-111 FITC.

Product Details

Summary
Reactivity Hu, Rt, BvSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
MEM-111
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

ICAM-1/CD54 Antibody (MEM-111) Summary

Immunogen
Raji human Burkitt's lymphoma cell line (NM_000201.2).
Specificity
The antibody MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Flow Cytometry 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • CyTOF-ready
Application Notes
WB: Use under non-reducing conditions. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26256764)
Positive Control
ICAM-1/CD54 Lysate (NBL1-11804)
Publications
Read Publications using
NB500-318 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for ICAM-1/CD54 Antibody (MEM-111)

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

CD54 (ICAM1) is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase in inflammation. Also, ICAM1 is detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (NB500-318)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ICAM-1/CD54 Antibody (NB500-318). (Showing 1 - 2 of 2 FAQs).

  1. Is the antibody kappa chain specific?
    • Our product NB500-318 is isotype IgG2a kappa.
  2. The vial says to store at -20C but the data sheet says to store at 4C. Which is correct?
    • The correct storage temperature for NB500-318 is 4C.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-318AF405
Alexa Fluor 488 Labeled NB500-318AF488
Alexa Fluor 647 Labeled NB500-318AF647
Alexa Fluor 700 Labeled NB500-318AF700
Allophycocyanin Labeled NB500-318APC
DyLight 350 Labeled NB500-318UV
DyLight 405 Labeled NB500-318V
DyLight 488 Labeled NB500-318G
DyLight 680 Labeled NB500-318FR
DyLight 755 Labeled NB500-318IR
FITC Labeled NB100-77927
PE Labeled NB500-318PE
PerCP Labeled NB500-318PCP

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Bioinformatics Tool for ICAM-1/CD54 Antibody (NB500-318)

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Diseases for ICAM-1/CD54 Antibody (NB500-318)

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Bioinformatics

Gene Symbol ICAM1
Entrez