ICAM-1/CD54 Antibody (166623) [Phycoerythrin]

Mouse splenocytes were stained with Rat Anti-Mouse ICAM-1/CD54 PE-conjugated Monoclonal Antibody (Catalog # FAB796P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 166623
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

ICAM-1/CD54 Antibody (166623) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
  Gln28-Asn485
  Accession # P13597
• Specificity: Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and recombinant mouse (rm) ICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, recombinant rat (rr) ICAM-1, rmMAdCAM-1, or rmVCAM-1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: ICAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (166623) [Phycoerythrin]

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

ICAM-1 (InterCellular Adhesion Molecule-1), also known as CD54, is an 85-93 kDa member of the Immunoglobulin supergene family of molecules. Mouse ICAM-1 is a 510 amino acid (aa) type I transmembrane glycoprotein that contains five C2-type Ig-like domains in a 458 aa extracellular domain (ECD) (aa 28-485). It is expressed constitutively by, or inducibly on, a very wide variety of cells, including neurons, dendritic cells, fibroblasts, skeletal muscle myocytes, keratinocytes, eosinophils, neutrophils, hepatocytes, monocytes and vascular endothelial cells. On the cell surface, it exists primarily as an "intermediate" between a homodimeric and monomeric state. The homodimeric pairing does not seem to offer a distinct advantage with respect to ligand binding. On the other hand, the degree of glycosylation has a marked impact on ICAM-1 ligand binding, with under-glycosylation associated with low affinity ligand binding. Ligands for ICAM-1 include LFA-1 ( alpha L beta 2), Mac-1 ( alpha M beta 2), CD11c/CD18, fibrinogen, hyaluronan and select rhinoviruses. Notably, ICAM-1 is recognized to be a potential coreceptor for HGF, acting in concert with c-Met during ligand binding. ICAM-1 is best known for its role in white cell trafficking (leukocyte binding to endothelium), and is also reported to play a key role in the establishment of CD8⁺ T cell memory, serving as a link between mature dendritic cells and naïve CD8⁺ T cells. Over its ECD, mouse ICAM-1 shares 78% and 54% aa sequence identity with rat and human ICAM-1 ECD, respectively. There is one isoform variant for mouse ICAM-1 listed in SwissProt that shows a 28 aa substitution for aa 1-34.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-1/CD54 Antibody (FAB796P)(1)

Publication using FAB796P

• Norton , James E, Lytle , Andrew G, Shen , Shixue, Tzvetkov , Evgeni P, Dorfmeier , Corin L, McGettigan , James P ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo. PLoS ONE, 2014-01-29;9(1):e87098. 2014-01-29 [PMID: 24489846] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: ICAM1
• Uniprot:
  • P13597()