ICAM-1/CD54 Antibody (141032) [Fluorescein]

Rat splenocytes were stained with Mouse Anti-Rat ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5831F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Flow
• Clone: 141032
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

ICAM-1/CD54 Antibody (141032) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat ICAM‑1/CD54
  Gln28-Thr493 (predicted)
  Accession # Q00238
• Specificity: Detects rat ICAM‑1/CD54 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) DCC, recombinant human (rh) ICAM-1, rmICAM-1, rmICAM-2, rhICAM-3, rmICAM-5, rmMAdCAM-1, rhCD31 or rmVCAM-1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (141032) [Fluorescein]

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 ( alpha L/ beta 2 or CD11a/CD18) and Mac-1 ( alpha M/ beta 2 or CD11b/CD18). ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta , and LPS. Within the extracellular domain, rat ICAM-1 shares 53% and 78% amino acid sequence identity with human and mouse ICAM-1, respectively. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
2. Siu, G. et al. (1989) J. Immunol. 143:3813.
3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-1/CD54 Antibody (FAB5831F)(3)

Publications using FAB5831F

• Kim A. Luetchford, Julian B. Chaudhuri, Paul A. De Bank Silk fibroin/gelatin microcarriers as scaffolds for bone tissue engineering Materials Science and Engineering: C 1/1/2020 [PMID: 31753329]
• S Tarantini, MN Valcarcel-, P Toth, A Yabluchans, Z Tucsek, T Kiss, P Hertelendy, M Kinter, P Ballabh, Z Süle, E Farkas, JA Baur, DA Sinclair, A Csiszar, Z Ungvari Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice Redox Biol, 2019-04-10;24(0):101192. 2019-04-10 [PMID: 31015147] (Flow Cytometry, Rat)
• Leslie A. Blair, April K. Haven, Natalie N. Bauer Circulating microparticles in severe pulmonary arterial hypertension increase intercellular adhesion molecule-1 expression selectively in pulmonary artery endothelium Respiratory Research 12/1/2016 [PMID: 27765042]

Bioinformatics

• Gene Symbol: ICAM1
• Uniprot:
  • Q00238()