ICAM-1/CD54 Antibody (141017) [Unconjugated]

• Reactivity: Rt
• Applications: ELISA(Cap)
• Clone: 141017
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ICAM-1/CD54 Antibody (141017) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat ICAM-1
  Met1-Thr493
  Accession # Q00238
• Specificity: Detects rat ICAM-1 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CD31, recombinant mouse (rm) DCC, rhICAM-1, rmICAM-1, rmICAM-2, rhICAM-3, rhMAdCAM-1, or rmVCAM-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Rat ICAM-1/CD54 Biotinylated Antibody (Catalog number BAF583)
  Standard: Recombinant Rat ICAM-1/CD54 Fc Chimera (Catalog number 583-IC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (141017) [Unconjugated]

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
2. Siu, G. et al. (1989) J. Immunol. 143:3813.
3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-1/CD54 Antibody (MAB5832)(2)

Publications using MAB5832

• Yifeng Zhao, Limin Zhang, Rui Han, Yonghua Si, Zigang Zhao Intravenous injection of post‑hemorrhagic shock mesenteric lymph induces multiple organ injury in rats Experimental and Therapeutic Medicine 12/5/2018 [PMID: 30680027]
• Stacy Hu, Steven Koevary Efficacy of Antibody Delivery to the Retina and Optic Nerve by Topical Administration Journal of Ocular Pharmacology and Therapeutics 2016-05-01 [PMID: 26771217]

Bioinformatics

• Gene Symbol: ICAM1
• Uniprot:
  • Q00238()