iASPP Antibody

Images

 
Western Blot: iASPP Antibody [NBP1-77912] - Shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band ...read more
Immunohistochemistry: iASPP Antibody [NBP1-77912] - Staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

iASPP Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 780-797 of human iASPP (isoform 1) protein.
Specificity
This antibody is directed against the human iASPP protein. A BLAST analysis was used to suggest cross reactivity with iASPP proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1R13L
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:40000-1:160000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect bands approximately 100 kDa and 50 kDa in size corresponding - isoforms 1 and 2 respectively of iASPP protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for iASPP Antibody

  • IASPPinhibitor of apoptosis stimulating protein of p53
  • Inhibitor of ASPP protein
  • NFkB-interacting protein 1
  • NKIP1
  • PPP1R13BL
  • PPP1R13B-like protein
  • Protein iASPP
  • protein phosphatase 1, regulatory (inhibitor) subunit 13 like
  • protein phosphatase 1, regulatory subunit 13 like
  • RAINFkB interacting protein 1
  • relA-associated inhibitor

Background

ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PPP1R13L
Uniprot