PAF49 Antibody - BSA Free

Western Blot: PAF49 Antibody [NBP2-19676] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PAF49 Antibody [NBP2-19676] - Paraffin-embedded human ovarian cancer. PAF49 antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PAF49 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PAF49 Antibody - BSA Free (NBP2-19676) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.
• Localization: Nucleus; nucleolus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD3EAP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PAF49 Antibody - BSA Free

• A34.5
• antisense to ERCC 1
• Antisense to ERCC-1 protein
• ASE-1ASE1
• CASTMGC118851
• CD3 epsilon associated protein
• CD3e antigen, epsilon polypeptide associated protein
• CD3e molecule, epsilon associated protein
• CD3E-associated protein
• CD3-epsilon-associated protein
• DNA-directed RNA polymerase I subunit RPA34
• PAF49RNA polymerase I-associated factor PAF49

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3EAP
• Uniprot:
  • O15446(Human)