HUS1 Antibody (2B12) - Azide and BSA Free

Immunoprecipitation: HUS1 Antibody (2B12) [H00003364-M01] - Analysis of HUS1 transfected lysate using anti-HUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HUS1 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: HUS1 Antibody (2B12) [H00003364-M01] - Detection limit for recombinant GST tagged HUS1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA, IP
• Clone: 2B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HUS1 Antibody (2B12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HUS1 (AAH07013, 1 a.a. ~ 280 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS
• Specificity: HUS1 checkpoint homolog (S. pombe)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HUS1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
• Application Notes: Antibody reactivity against recombinant protein on ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HUS1 Antibody (2B12) - Azide and BSA Free

• checkpoint protein HUS1
• hHUS1
• HUS1 (S. pombe) checkpoint homolog
• HUS1 checkpoint homolog (S. pombe)
• hus1+-like protein

Background

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HUS1
• Entrez:
  • 3364(Human)
• OMIM:
  • 603760(Human)
• Uniprot:
  • AAH07013(Human)