Huntingtin Antibody

Images

 
Western Blot: Huntingtin Antibody [NBP1-44265] - Lane 1: mouse brain extract. Lane 2: mouse brain extract with blocking peptide. Load: 10 ug per lane. Primary antibody: Huntingtin antibody at 0.1ug/mL for overnight at ...read more
Western Blot: Huntingtin Antibody [NBP1-44265] - Western blot analysis on mouse brain extract using 0.1ug/mL of NBP1-44265 (lane 1) and 0.1ug/mL of NBP1-44265 combined with 1ug/mL of blocking peptide (lane 2).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Order Details

Huntingtin Antibody Summary

Immunogen
Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington.
Specificity
This antibody is specific to HTT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTT
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/ml
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100-1:500

Reactivity Notes

This antibody is reactive in Human, Mouse and Rat

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
Preservative
No Preservative
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Huntingtin Antibody

  • HD protein
  • huntingtin (Huntington disease)
  • huntingtin
  • IT15HDHuntington disease protein

Background

Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HTT
Entrez
Uniprot