Humanin Antibody

Images

 
Western Blot: Humanin Antibody [NB100-56877] - Analysis of Humanin in (A) recombinant fusion protein containing amino acids 1-24 and (B) fusion partner without these amino acids, using Humanin antibody at 1:2000.
Immunohistochemistry-Paraffin: Humanin Antibody [NB100-56877] - Analysis Humanin in human testis at 1:100.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Humanin Antibody Summary

Immunogen
The full length peptide sequence from the human MT-RNR2 protein (accesion number Q8IVG9).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTRNR2L1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:500-1:2000
Application Notes
ICC data from customer review.
Reviewed Applications
Read 1 Review rated 3
using
NB100-56877 in the following applications:

Publications
Read Publication using NB100-56877.

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Alternate Names for Humanin Antibody

  • HN
  • HUNIN

Background

Humanin is a novel 24 A.A polypeptide identified in Alzheimer's disease-affected brain. It is 99% homologous to mitochondrial 16s ribosomal RNA and functions as a novel neuroprotective and cytoprotective factor. It specifically inhibits neuronal cell death induced by beta-amyloids and Alzheimer's disease causing FAD genes by competitively inhibiting access of FPRL1 receptor to beta-amyloids. It acts as an anti-apoptotic factor by binding to Bcl-2/Bax family of pro-apoptotic factors including Bax, Bak, Bid and BimEL and inhibits their pro-apoptotic activity. It activates JAK/STAT signaling pathway and is regulated by TRIM11. It interacts with IGFBP-3 and specifically blocks IGFBP-3 induced cell death in glioblastoma cells. Humanin is also expressed in colon and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Humanin Antibody (NB100-56877)(1)


Showing Publication 1 - 1 of 1.
Publication using NB100-56877 Applications Species
Celvin B Prevention of growth failure caused by glucocorticoids and inflammation Thesis

Review for Humanin Antibody (NB100-56877) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56877:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Humanin NB100-56877
Enlarge
3
reviewed by:
Manjari Dimri
IF Human 05/02/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedcells
SpeciesHuman
LotCS032105A-02

Blocking

Blocking DetailsBlocked with 1%BSA in PBS for 1hr

Primary Anitbody

Dilution Ratio1:500 dilution in blocking buffer for 1hr at room temp

Secondary Antibody

Secondary DescriptionAlexa 488 donkey anti-rabbit
Secondary Concentration1:2000 dilution

Details

Fixation DetailsFixed with 4% formaldehyde for 20min at room temp, permeabilized with 0.5% triton in PBS for 5 min
Wash Descriptionwash 3 times with PBS 8 min each wash

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Humanin Antibody (NB100-56877) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Humanin Products

Array NB100-56877

Bioinformatics Tool for Humanin Antibody (NB100-56877)

Discover related pathways, diseases and genes to Humanin Antibody (NB100-56877). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on Humanin

There are no specific blogs for Humanin, but you can read our latest blog posts.
Omicron Variant Antibodies

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Contact Information

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Product PDFs

Calculators

Concentration Calculator

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Recent Reviews

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1
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Manjari Dimri
05/02/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol MTRNR2L1
Uniprot