Pyridoxal Kinase/PDXK Antibody (OTI5H5)

Western Blot: PDXK Antibody (5H5) [NBP2-00821] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDXK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDXK.

Immunocytochemistry/Immunofluorescence: PDXK Antibody (5H5) [NBP2-00821] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDXK.

Immunohistochemistry-Paraffin: PDXK Antibody (5H5) [NBP2-00821] - Staining of paraffin-embedded Human pancreas tissue using anti-PDXK mouse monoclonal antibody.

Flow Cytometry: PDXK Antibody (5H5) [NBP2-00821] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDXK antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: PDXK Antibody (5H5) [NBP2-00821] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDXK mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDXK Antibody (5H5) [NBP2-00821] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDXK mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDXK Antibody (5H5) [NBP2-00821] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDXK mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDXK Antibody (5H5) [NBP2-00821] - Staining of paraffin-embedded Human endometrium tissue using anti-PDXK mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDXK Antibody (5H5) [NBP2-00821] - Staining of paraffin-embedded Human lymph node tissue using anti-PDXK mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI5H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.82 mg/ml

Pyridoxal Kinase/PDXK Antibody (OTI5H5) Summary

• Description: Novus Biologicals Mouse Pyridoxal Kinase/PDXK Antibody (OTI5H5) (NBP2-00821) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PDXK(NP_03672) produced in HEK293 cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDXK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 34.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.82 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pyridoxal Kinase/PDXK Antibody (OTI5H5)

• C21orf124
• C21orf97
• chromosome 21 open reading frame 124
• DKFZp566A071
• EC 2.7.1.35
• FLJ21324
• FLJ37311
• HEL-S-1a
• MGC15873
• MGC31754
• MGC52346
• PDXK
• PKH
• PKHchromosome 21 open reading frame 97
• PNK
• PNKhuman pyridoxal kinase, EC 2.7.1.3510FLJ31940
• PRED79
• pyridoxal (pyridoxine, vitamin B6) kinase
• Pyridoxal Kinase
• pyridoxamine kinase
• Pyridoxine kinase
• Vitamin B6 Kinase

Background

The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDXK
• Entrez:
  • 8566(Human)