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Human VEGF DuoSet ELISA, 15 Plate


Effects of cell stress on VEGF-A and HIF1 alpha expression in Müller cells and Y79 photoreceptors. (A) VEGF-A was measured by ELISA using conditioned media collected from MIO-M1 and Y79 cells, n = 4–5/group in more

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Reactivity HuSpecies Glossary
Applications ELISA

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Human VEGF DuoSet ELISA, 15 Plate Summary

Assay Type
Solid Phase Sandwich ELISA
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See PDF Datasheet for details
Spike Recovery
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Sample Volume
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Application Notes
No significant interference observed with available related molecules.
Reviewed Applications
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DY293B in the following application:

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DY293B in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human VEGF DuoSet ELISA, 15 Plate

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of antiparallel disulfide-linked dimers (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha (3, 4, 9, 16). VEGF isoforms are differentially expressed during development and in the adult (3). 
VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR), and induce their homodimerization and autophosphorylation (3, 4, 7, 17, 18). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (19). 
VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size, but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (20-22). After birth, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (23, 24). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (25). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (26).

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Publications for VEGF (DY293B)(183)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Ovine, Porcine, Xenograft.

We have publications tested in 2 applications: ChIP, ELISA.

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Showing Publications 1 - 10 of 183. Show All 183 Publications.
Publications using DY293B Applications Species
Rehage, E;Sowislok, A;Busch, A;Papaeleftheriou, E;Jansen, M;Jäger, M; Surgical Site-Released Tissue Is Potent to Generate Bone onto TCP and PCL-TCP Scaffolds In Vitro International journal of molecular sciences 2023-11-01 [PMID: 37958857] (Human) Human
Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S; CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy International journal of molecular sciences 2023-10-25 [PMID: 37958563] (Human) Human
Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D; Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial PloS one 2023-11-02 [PMID: 37917760] (Human) Human
Skovronova, R;Scaccia, E;Calcat-I-Cervera, S;Bussolati, B;O'Brien, T;Bieback, K; Adipose stromal cells bioproducts as cell-free therapies: manufacturing and therapeutic dose determine in vitro functionality Journal of translational medicine 2023-10-16 [PMID: 37840135] (Human) Human
Chung, IC;Huang, WC;Huang, YT;Chen, ML;Tsai, AW;Wu, PY;Yuan, TT; Unrevealed roles of extracellular enolase?1 (ENO1) in promoting glycolysis and pro?cancer activities in multiple myeloma via hypoxia?inducible factor 1? Oncology reports 2023-11-01 [PMID: 37800625] (Human, Mouse) Human, Mouse
Park, GT;Lim, JK;Choi, EB;Lim, MJ;Yun, BY;Kim, DK;Yoon, JW;Hong, YG;Chang, JH;Bae, SH;Ahn, JY;Kim, JH; Transplantation of adipose tissue-derived microvascular fragments promotes therapy of critical limb ischemia Biomaterials research 2023-07-16 [PMID: 37455318] (Human) Human
Shams, S;Stilhano, RS;Silva, EA; Harnessing EGLN1 Gene Editing to Amplify HIF-1 alpha and Enhance Human Angiogenic Response bioRxiv : the preprint server for biology 2023-05-30 [PMID: 37398294] (Human) Human
Blangero, F;Robert, M;Andraud, T;Dumontet, C;Vidal, H;Eljaafari, A; Contribution of Mesenchymal Stem Cells from Obese Adipose Tissue to PD-L1 Over-Expression and Breast Cancer Progression through Pathogenic Th17 Cell Activation Cancers 2023-05-29 [PMID: 37296927] (Human) Human
M Minoves, F Hazane-Puc, G Moriondo, A Boutin-Par, E Lemarié, JL Pépin, D Godin-Ribu, A Briançon-M Differential Impact of Intermittent vs. Sustained Hypoxia on HIF-1, VEGF and Proliferation of HepG2 Cells International Journal of Molecular Sciences, 2023-04-07;24(8):. 2023-04-07 [PMID: 37108039] (ELISA, Human) ELISA Human
D Sikorska, D Kami?ska, R Catar, D Wu, H Zhao, P Wang, J Kamhieh-Mi, M Banasik, M Kusztal, M Cielecka, M Zakliczy?s, R Rutkowski, K Korybalska, H Heidecke, G Moll, W Samborski Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study Journal of Clinical Medicine, 2023-03-16;12(6):. 2023-03-16 [PMID: 36983302] (Human) Human
Show All 183 Publications.

Review for VEGF (DY293B) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using DY293B:
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Sample Testedhuman dermal fibroblast,Supernatant from cultured human dermal fibroblasts (HDFa)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF (DY293B). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Additional VEGF Products

Blogs on VEGF. Showing 1-10 of 23 blog posts - Show all blog posts.

Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

Showing 1-10 of 23 blog posts - Show all blog posts.

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Gene Symbol VEGFA