Human VE-Cadherin Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human VE-Cadherin Quantikine ELISA Kit Summary

• Background: The Quantikine Human VE-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VE-Cadherin in cell culture supernates, serum, and plasma. It contains NS0-derived recombinant human VE-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VE-Cadherin showed linear curves that were parallel to the standard curve...s obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VE-Cadherin.
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• Specificity: Natural and recombinant human VE-Cadherin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CDH5

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human VE-Cadherin Quantikine ELISA Kit

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2⁺-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VE-Cadherin (DCADV0)(5)

Publications using DCADV0

• Prabhakar, A;Kumar, R;Wadhwa, M;Ghatpande, P;Zhang, J;Zhao, Z;Lizama, CO;Kharbikar, BN;Gräf, S;Treacy, CM;Morrell, NW;Graham, BB;Lagna, G;Hata, A; Reversal of pulmonary veno-occlusive disease phenotypes by inhibition of the integrated stress response bioRxiv : the preprint server for biology 2023-11-28 [PMID: 38076809] (Human, Rat)
• PN Kouam, GA Rezniczek, IA Adamietz, H Bühler Ionizing radiation increases the endothelial permeability and the transendothelial migration of tumor cells through ADAM10-activation and subsequent degradation of VE-cadherin BMC Cancer, 2019-10-16;19(1):958. 2019-10-16 [PMID: 31619190] (Human)
• NJ Aachmann-A, SJ Christense, K Lisbjerg, P Oturai, PI Johansson, NH Holstein-R, NV Olsen Recombinant erythropoietin acutely decreases renal perfusion and decouples the renin-angiotensin-aldosterone system Physiol Rep, 2018-03-01;6(5):. 2018-03-01 [PMID: 29504258] (Human)
• P Polzik, PI Johansson, O Hyldegaard How biomarkers reflect the prognosis and treatment of necrotising soft tissue infections and the effects of hyperbaric oxygen therapy: the protocol of the prospective cohort PROTREAT study conducted at a tertiary hospital in Copenhagen, Denmark BMJ Open, 2017-10-05;7(10):e017805. 2017-10-05 [PMID: 28982834] (Human)
• S Suwarto, RT Sasmono, R Sinto, E Ibrahim, M Suryamin Association of Endothelial Glycocalyx and Tight and Adherens Junctions With Severity of Plasma Leakage in Dengue Infection J. Infect. Dis., 2017-03-15;215(6):992-999. 2017-03-15 [PMID: 28453844] (Human)

FAQs for VE-Cadherin (DCADV0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CDH5