Human TIM-3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TIM-3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human TIM-3 Immunoassay is a 2.5 hour solid phase ELISA designed to measure TIM-3 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human TIM-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TIM-3 showed linear curves that were parallel to the standard curves o...btained using the Quantikine Human TIM-3 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human TIM-3.
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• Specificity: Natural and recombinant human TIM-3.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: HAVCR2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TIM-3 Quantikine ELISA Kit

• CD366
• FLJ14428
• HAVCR2
• HAVcr-2
• hepatitis A virus cellular receptor 2
• kidney injury molecule-3
• KIM-3
• SPTCL
• T cell immunoglobulin mucin 3
• T cell immunoglobulin mucin-3
• T-cell immunoglobulin and mucin domain-containing protein 3
• TIM 3
• TIM3 T-cell membrane protein 3
• TIM3
• TIM-3
• TIMD3
• TIMD-3
• TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2 is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. It is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). TIM-3 ligation by Galectin-9 attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. Dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. TIM-3 also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TIM-3 (DTIM30)(3)

Publications using DTIM30

• S Valente, C Nascimento, A Gameiro, J Ferreira, J Correia, F Ferreira TIM-3 Is a Potential Immune Checkpoint Target in Cats with Mammary Carcinoma Cancers, 2023-01-06;15(2):. 2023-01-06 [PMID: 36672332] (Human)
• H Matsumoto, Y Fujita, T Asano, N Matsuoka, J Temmoku, S Sato, M Yashiro-Fu, K Yokose, S Yoshida, E Suzuki, T Yago, H Watanabe, A Kawakami, K Migita Association between inflammatory cytokines and immune-checkpoint molecule in rheumatoid arthritis PLoS ONE, 2021-11-18;16(11):e0260254. 2021-11-18 [PMID: 34793561] (Human)
• J Gao, X Qiu, X Li, H Fan, F Zhang, T Lv, Y Song Expression profiles and clinical value of plasma exosomal Tim-3 and Galectin-9 in non-small cell lung cancer Biochem. Biophys. Res. Commun., 2018-02-13;0(0):. 2018-02-13 [PMID: 29452091] (Human)

Bioinformatics

• Gene Symbol: HAVCR2