Human Tie-2 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Tie-2 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TEK

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Tie-2 DuoSet ELISA, 15 Plate

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

Tie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult endothelial cells, hematopoietic stem cells, and a circulating population of proangiogenic monocytes. Tie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular integrity by mediating endothelial cell-smooth muscle cell communication and inhibiting endothelial cell apoptosis. It is also required for embryonic development of the endocardium. In addition, Tie-2 signaling mediates the quiescence of bone marrow stem cells in response to osteoblast-produced Angiopoietin-1. This quiescence is critical for maintaining an ongoing hematopoietic capability in the bone marrow.

Publications for Tie-2 (DY5159)(11)

Publications using DY5159

• Varo, R;Sitoe, A;Madrid, L;Aide, P;Cuamba, I;Cossa, A;Jairoce, C;Barrios, D;Martiáñez-Vendrell, X;Balanza, N;Cisteró, P;Ouchi, D;Quintó, L;Crowley, VM;Gupta, H;Kain, KC;Mayor, A;Bassat, Q; Host biomarkers and parasite biomass are associated with severe malaria in Mozambican children: a case-control study Scientific reports 2025-04-24 [PMID: 40275018] (Human)
• P Ku?, R Charkiewic, D Klasa-Mazu, T Milczek, B Mroczko, J Nikli?ski, P Lauda?ski Profiling of selected angiogenesis-related genes in serous ovarian cancer patients Adv Med Sci, 2017-02-21;62(1):116-120. 2017-02-21 [PMID: 28235714] (Human)
• Erdman L, D'Acremont V, Hayford K, Rajwans N, Kilowoko M, Kyungu E, Hongoa P, Alamo L, Streiner D, Genton B, Kain K Biomarkers of Host Response Predict Primary End-Point Radiological Pneumonia in Tanzanian Children with Clinical Pneumonia: A Prospective Cohort Study. PLoS ONE, 2015-09-14;10(9):e0137592. 2015-09-14 [PMID: 26366571] (Human)
• Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta. PLoS Negl Trop Dis, 2015-06-19;9(6):e0003824. 2015-06-19 [PMID: 26090803] (Human)
• Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-01-20;14(0):35. 2014-01-20 [PMID: 24444080] (Human)
• Giri H, Chandel S, Dwarakanath L, Sreekumar S, Dixit M Increased endothelial inflammation, sTie-2 and arginase activity in umbilical cords obtained from gestational diabetic mothers. PLoS ONE, 2013-12-20;8(12):e84546. 2013-12-20 [PMID: 24376824] (Human)
• Conroy AL, Liles WC, Molyneux ME Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. PLoS ONE, 2011-12-12;6(12):e28540. 2011-12-12 [PMID: 22174834] (Human)
• Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study. PLoS ONE, 2011-02-25;6(2):e17440. 2011-02-25 [PMID: 21364762] (Human)
• Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study. PLoS ONE, 2010-12-29;5(12):e15291. 2010-12-29 [PMID: 21209923] (Human)
• Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy. Placenta, 2010-03-24;31(6):545-8. 2010-03-24 [PMID: 20338637] (Human)
• Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H Serum levels of angiogenic factors and their prognostic relevance in bladder cancer. Pathol. Oncol. Res., 2008-09-20;15(2):193-201. 2008-09-20 [PMID: 18807212] (Human)

FAQs for Tie-2 (DY5159). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TEK