Human Phospho-AMPK alpha 1 (T183) DuoSet IC ELISA, 2 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Phospho-AMPK alpha 1 (T183) DuoSet IC ELISA, 2 Plate Summary

• Modification: p Thr183
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: PRKAA1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-AMPK alpha 1 (T183) DuoSet IC ELISA, 2 Plate

• AMP -activate kinase alpha 1 subunit
• AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
• AMPK alpha 1
• AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
• AMPK subunit alpha-1
• AMPK
• AMPK1
• AMPKa1
• EC 2.7.11
• EC 2.7.11.1
• MGC33776
• MGC57364
• PRKAA1
• protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic a subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms (alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations capable of forming complexes. The catalytic a subunit of AMPK is activated allosterically by AMP and by phosphorylation via the AMPK kinase LKB1. Active AMPK downregulates anabolic pathways such as fatty acid, triglyceride, and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation. AMPK's role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Publications for AMPK alpha 1 (DYC3528-2)(4)

Publications using DYC3528-2

• Zhong, S;Wang, R;Gao, X;Guo, Q;Lin, R;Luo, M; Modular DNA barcoding of nanobodies enables multiplexed in situ protein imaging and high-throughput biomolecule detection eLife 2025-07-22 [PMID: 40694500] (Human)
• Ghandour, F;Kassem, S;Simanovich, E;Rahat, M; Glucose Promotes EMMPRIN/CD147 and the Secretion of Pro-Angiogenic Factors in a Co-Culture System of Endothelial Cells and Monocytes Biomedicines 2024-03-22 [PMID: 38672062] (Human)
• Y Chen, F Qiu, B Yu, Y Chen, F Zuo, X Zhu, KS Nandakumar, C Xiao Metformin, an AMPK Activator, Inhibits Activation of FLSs but Promotes HAPLN1 Secretion Mol Ther Methods Clin Dev, 2020-05-13;17(0):1202-1214. 2020-05-13 [PMID: 32518807] (Human)
• S Adeberg, D Bernhardt, SB Harrabi, NH Nicolay, J Hörner-Rie, L König, M Repka, A Mohr, A Abdollahi, KJ Weber, J Debus, S Rieken Metformin Enhanced in Vitro Radiosensitivity Associates with G2/M Cell Cycle Arrest and Elevated Adenosine-5'-monophosphate-activated Protein Kinase Levels in Glioblastoma Radiol Oncol, 2017-11-01;51(4):431-437. 2017-11-01 [PMID: 29333122] (Human)

FAQs for AMPK alpha 1 (DYC3528-2). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: PRKAA1