Human PDGF-BB DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human PDGF-BB DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: PDGFB

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human PDGF-BB DuoSet ELISA, 15 Plate

• c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV
• PDGFBB
• PDGF-BB

Background

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Publications for PDGF-BB (DY220)(28)

Publications using DY220

• Timmer, KB;Killian, ML;Harley, BAC; Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair Bioactive materials 2025-11-01 [PMID: 40703334] (Human)
• Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P; Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders Psychoneuroendocrinology 2024-07-06 [PMID: 39003840] (Human)
• Jiang, J;Röper, L;Fuchs, F;Hanschen, M;Failer, S;Alageel, S;Cong, X;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P; Bone Regenerative Effect of Injectable Hypoxia Preconditioned Serum-Fibrin (HPS-F) in an Ex Vivo Bone Defect Model International journal of molecular sciences 2024-05-13 [PMID: 38791352] (Human)
• Miguel-Pastor, L;Satué, K;Chicharro, D;Damiá, E;Cuervo, B;Torres-Torrillas, M;Martins, E;Velasco-Martínez, MG;Carrillo, JM;Sopena, JJ;Cerón, JJ;Rubio, M; Characterization of platelet rich plasma in feline immunodeficiency virus-infected cats: Cell, and PDGF-BB and TGF-beta 1 growth factor analysis Research in veterinary science 2024-01-06 [PMID: 38218061] (Feline)
• Jiang, J;Altammar, J;Cong, X;Ramsauer, L;Steinbacher, V;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P; Hypoxia Preconditioned Serum (HPS) Promotes Proliferation and Chondrogenic Phenotype of Chondrocytes In Vitro International journal of molecular sciences 2023-06-21 [PMID: 37445617] (Human)
• J Xu, S Fahmy-Garc, MA Wesdorp, N Kops, L Forte, C De Luca, MM Misciagna, L Dolcini, G Filardo, M Labberté, K Vancíková, J Kok, B van Rietbe, J Nickel, E Farrell, PAJ Brama, GJVM van Osch Effectiveness of BMP-2 and PDGF-BB Adsorption onto a Collagen/Collagen-Magnesium-Hydroxyapatite Scaffold in Weight-Bearing and Non-Weight-Bearing Osteochondral Defect Bone Repair: In Vitro, Ex Vivo and In Vivo Evaluation Journal of functional biomaterials, 2023-02-16;14(2):. 2023-02-16 [PMID: 36826910] (Human)
• J Jiang, X Cong, S Alageel, U Dornseifer, AF Schilling, E Hadjipanay, HG Machens, P Moog In Vitro Comparison of Lymphangiogenic Potential of Hypoxia Preconditioned Serum (HPS) and Platelet-Rich Plasma (PRP) International Journal of Molecular Sciences, 2023-01-19;24(3):. 2023-01-19 [PMID: 36768283] (Human)
• H Lena, G David, K Johann, J Yvonne, Z Frank G, H Daniela, B Roman, B Rolf E, R Nicole In situ regeneration of nasal septal defects using acellular cartilage enhanced with platelet-derived growth factor Journal of tissue engineering, 2022-09-20;13(0):2041731422111. 2022-09-20 [PMID: 36158899] (Porcine)
• K Zwittnig, B Kirnbauer, N Jakse, P Schlenke, I Mischak, S Ghanaati, S Al-Maawi, D Végh, M Payer, TA Zrnc Growth Factor Release within Liquid and Solid PRF Journal of Clinical Medicine, 2022-08-29;11(17):. 2022-08-29 [PMID: 36078998] (Human)
• AL Graça, RMA Domingues, I Calejo, M Gómez-Flor, ME Gomes Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model International Journal of Molecular Sciences, 2022-03-09;23(6):. 2022-03-09 [PMID: 35328370] (Human)
• S Hakki, EJ Robinson, MG Robson Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula Scientific Reports, 2022-01-26;12(1):1427. 2022-01-26 [PMID: 35082332] (Human)
• SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 2022-01-14;23(2):. 2022-01-14 [PMID: 35055054] (Human)
• PC Duque-Madr, J Velasco-Bo, A Ceballos-M, C López, JU Carmona Intramammary treatment using allogeneic pure platelet-rich plasma in cows with subclinical mastitis caused by Gram-positive bacteria Scientific Reports, 2021-12-09;11(1):23737. 2021-12-09 [PMID: 34887474] (Bovine)
• P Niemiec, K Szyluk, A Balcerzyk, M Kalita, A Jarosz, J Iwanicka, T Iwanicki, T Nowak, M Negru, T Francuz, W Garczorz, W Grzeszczak, S Górczy?ska, W Kania, I ?ak Why PRP works only on certain patients with tennis elbow? Is PDGFB gene a key for PRP therapy effectiveness? A prospective cohort study Bmc Musculoskeletal Disorders, 2021-08-18;22(1):710. 2021-08-18 [PMID: 34407802] (Human)
• M Tutter, C Schug, KA Schmohl, S Urnauer, C Kitzberger, N Schwenk, M Petrini, C Zach, S Ziegler, P Bartenstei, WA Weber, G Multhoff, E Wagner, LH Lindner, PJ Nelson, C Spitzweg Regional hyperthermia enhances mesenchymal stem cell recruitment to tumor stroma: Implications for mesenchymal stem cell-based tumor therapy Mol Ther, 2020-10-15;0(0):. 2020-10-15 [PMID: 33068779] (Human)
• M Aliabouzar, A Jivani, X Lu, OD Kripfgans, JB Fowlkes, ML Fabiilli Standing wave-assisted acoustic droplet vaporization for single and dual payload release in acoustically-responsive scaffolds Ultrason Sonochem, 2020-03-26;66(0):105109. 2020-03-26 [PMID: 32248042] (N/A)
• T Emi, K Michaud, E Orton, G Santilli, C Linh, M O'Connell, F Issa, S Kennedy Ultrasonic Generation of Pulsatile and Sequential Therapeutic Delivery Profiles from Calcium-Crosslinked Alginate Hydrogels Molecules, 2019-03-16;24(6):. 2019-03-16 [PMID: 30884862] (Human)
• RF Jiménez-Ar, C López, ME Álvarez, C Giraldo, M Prades, JU Carmona Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols Cytokine, 2017-06-23;97(0):149-155. 2017-06-23 [PMID: 28648869] (Equine)
• The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages Nat Commun, 2016-05-06;7(0):11385. 2016-05-06 [PMID: 27150562] (Human)
• DG Belair, NN Le, WL Murphy Regulating VEGF signaling in platelet concentrates via specific VEGF sequestering Biomater Sci, 2016-03-24;4(5):819-25. 2016-03-24 [PMID: 27010034] (Human)
• Giraldo C, Alvarez M, Carmona J Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res, 2015-03-14;11(0):60. 2015-03-14 [PMID: 25889052] (Equine)
• Giraldo C, Lopez C, Alvarez M, Samudio I, Prades M, Carmona J Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res, 2013-02-12;9(0):29. 2013-02-12 [PMID: 23402541] (Equine)
• Martino MM, Hubbell JA The 12th-14th type III repeats of fibronectin function as a highly promiscuous growth factor-binding domain. FASEB J., 2010-07-29;24(12):4711-21. 2010-07-29 [PMID: 20671107] (Human)
• Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity. Brain, 2010-07-21;133(0):2232-47. 2010-07-21 [PMID: 20659956] (Human)
• Peterson JE, Zurakowski D, Italiano JE Normal ranges of angiogenesis regulatory proteins in human platelets. Am. J. Hematol., 2010-07-01;85(7):487-93. 2010-07-01 [PMID: 20575035] (Human)
• Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment. FASEB J., 2010-05-19;24(10):3782-95. 2010-05-19 [PMID: 20484670] (Human)
• Chung I, Han G, Seshadri M, Gillard BM, Yu WD, Foster BA, Trump DL, Johnson CS Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo. Cancer Res., 2009-01-13;69(3):967-75. 2009-01-13 [PMID: 19141646] (Mouse)
• Ball SG, Shuttleworth CA, Kielty CM Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 2007-04-30;177(3):489-500. 2007-04-30 [PMID: 17470632] (Human)

FAQs for PDGF-BB (DY220). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PDGFB