Human PBEF/Visfatin DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human PBEF/Visfatin DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: NAMPT

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human PBEF/Visfatin DuoSet ELISA, 5 Plate

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

PBEF, also called Nampt or visfatin, is a ubiquitous 52 kDa nicotinamide phosphoribosyltransferase. PBEF is the rate-limiting component in the biosynthesis of NAD+, and functions in the cytoplasm to regulate energy metabolism during stress responses and immune activation. Although it lacks a signal sequence, PBEF appears to be secreted by visceral adipose and functions as a noncompetitive insulin mimetic. Mouse PBEF shows 96% and > 99% aa identity with human and rat PBEF, respectively.

Publications for PBEF/Visfatin/NAMPT (DY4335-05)(7)

Publications using DY4335-05

• R Jiménez-Es, D Vargas-Alc, P Flores-Esp, AC Helguera-R, O Villavicen, I Mancilla-H, C Irles, YD Torres-Ram, MY Valdespino, P Velázquez-, R Zamora-Esc, M Islas-Lópe, C Carranco-S, L Díaz, V Zaga-Clave, A Olmos-Orti High Glucose Promotes Inflammation and Weakens Placental Defenses against E. coli and S. agalactiae Infection: Protective Role of Insulin and Metformin International Journal of Molecular Sciences, 2023-03-09;24(6):. 2023-03-09 [PMID: 36982317] (Human)
• TH Chen, HC Hsu, JF You, CC Lai, YK Tsou, CL Hsu, CSJ Fann, RN Chien, ML Chang Extracellular Nicotinamide Phosphoribosyltransferase as a Surrogate Marker of Prominent Malignant Potential in Colonic Polyps: A 2-Year Prospective Study Cancers, 2023-03-10;15(6):. 2023-03-10 [PMID: 36980589] (Human)
• YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages Cancers, 2020-11-26;12(12):. 2020-11-26 [PMID: 33256011] (Human)
• A Bongrani, Y Elfassy, JS Brun, C Ramé, N Mellouk, S Fellahi, JP Bastard, R Levy, C Vasseur, P Froment, J Dupont Expression of adipokines in seminal fluid of men of normal weight Asian J. Androl., 2019-09-01;0(0):. 2019-09-01 [PMID: 31115360] (Human)
• M Tao, J Jiang, L Wang, Y Li, Q Mao, J Dong, J Zuo ?-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPT/NAD Controlled Inflammatory Reactions Evid Based Complement Alternat Med, 2018-10-10;2018(0):5470187. 2018-10-10 [PMID: 30405740] (Rat)
• C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis Peptides, 2018-02-21;102(0):31-37. 2018-02-21 [PMID: 29475075] (Human)
• SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype PLoS ONE, 2017-04-11;12(4):e0175109. 2017-04-11 [PMID: 28399156] (Human)

FAQs for PBEF/Visfatin/NAMPT (DY4335-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NAMPT