Human Neutrophil Elastase/ELA2 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Neutrophil Elastase/ELA2 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Neutrophil Elastase/ELA2 DuoSet ELISA, 5 Plate

• Bone marrow serine protease
• EC 3.4.21
• EC 3.4.21.37
• ELA2
• ELA2granulocyte-derived elastase
• ELANE
• elastase 2, neutrophil
• elastase, neutrophil expressed
• Elastase-2
• GE
• HLEelastase-2
• HNE
• Human leukocyte elastase
• Leukocyte Elastase
• Medullasin
• NE
• Neutrophil Elastase
• PMN elastase
• PMN-E
• polymorphonuclear elastase
• SCN1

Background

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Publications for Neutrophil Elastase/ELA2 (DY9167-05)(25)

Publications using DY9167-05

• Hyslop, K;Ki, KK;Naidoo, R;O'Brien, D;Prabhu, A;Gill, D;Anstey, C;Rapchuk, IL;McDonald, CI;Marshall, L;Pearse, I;Fraser, JF;Suen, JY;Passmore, MR; Cell-free mitochondrial DNA may predict the risk of post-operative complications and outcomes in surgical aortic valve replacement patients Scientific reports 2025-05-06 [PMID: 40328845] (Human)
• Da Silva Cunha, AL;Blanter, M;Renders, J;Gouwy, M;Lorent, N;Boon, M;Struyf, S;Carlon, MS; Inhibiting CFTR through inh-172 in primary neutrophils reveals CFTR-specific functional defects Scientific reports 2024-12-28 [PMID: 39732786] (Human)
• Hanford, HE;Price, CTD;Uriarte, S;Abu Kwaik, Y; Inhibition and evasion of neutrophil microbicidal responses by Legionella longbeachae mBio 2024-12-16 [PMID: 39679679] (Human)
• Mirandola, A;Kudriavtsev, A;Cofre Muñoz, CI;Navarro, RC;Macagno, M;Daoud, S;Sanchez, C;Pastor, B;Pisareva, E;Marin, MS;Ruiz, JG;Piris, A;Rodriguez, AG;Gonzalez, NS;Vivancos, A;Quarà, V;Mellano, A;Borghi, F;Corti, G;Marchiò, C;Sapino, A;Bartolini, A;Crisafulli, G;Bardelli, A;Di Maio, M;Lossaint, G;Frayssinoux, F;Crapez, E;Ychou, M;Soler, RS;Fenocchio, E;Fernandez Calotti, PX;Mazard, T;Vivas, CS;Elez, E;Di Nicolantonio, F;Thierry, AR; Post-surgery sequelae unrelated to disease progression and chemotherapy revealed in follow-up of patients with stage III colon cancer EBioMedicine 2024-09-19 [PMID: 39303668] (Human)
• Nicola, T;Wenger, N;Xu, X;Evans, M;Qiao, L;Rezonzew, G;Yang, Y;Jilling, T;Margaroli, C;Genschmer, K;Willis, K;Ambalavanan, N;Blalock, JE;Gaggar, A;Lal, CV; A lactobacilli-based inhaled live biotherapeutic product attenuates pulmonary neutrophilic inflammation Nature communications 2024-08-19 [PMID: 39160214] (Human)
• Das, S;Kaminski, TW;Schlegel, BT;Bain, W;Hu, S;Patel, A;Kale, SL;Chen, K;Lee, JS;Mallampalli, RK;Kagan, VE;Rajasundaram, D;McVerry, BJ;Sundd, P;Kitsios, GD;Ray, A;Ray, P; Neutrophils and galectin-3 defend mice from lethal bacterial infection and humans from acute respiratory failure Nature communications 2024-06-03 [PMID: 38830855] (Human)
• Ramakrishnan, G;Young, MK;Nayak, U;Rigo, I;Marrs, AS;Gilchrist, CA;Behm, BW;Madden, GR;Petri, WA; Systemic neutrophil degranulation and emergency granulopoiesis in patients with Clostridioides difficile infection Anaerobe 2024-03-19 [PMID: 38514010] (Human)
• Pastorek, M;Kone?ná, B;Janko, J;Janovi?ová, ?;Podracká, ?;Záhumenský, J;Šte?ová, E;Dúbrava, M;Hodosy, J;Vlková, B;Celec, P; Mitochondria-induced formation of neutrophil extracellular traps is enhanced in the elderly via Toll-like receptor 9 Journal of leukocyte biology 2023-08-30 [PMID: 37648664] (Human)
• Bakalovi?, G;Bokonji?, D;Mihajlovi?, D;?oli?, M;Mališ, V;Drakul, M;Tomi?, S;Joji?, I;Rako?evi?, S;Popovi?, D;Kozi?, L;Vasiljevi?, M;Beki?, M;Maši?, S;Ljuboja, O; Dysfunctions of Neutrophils in the Peripheral Blood of Children with Cystic Fibrosis Biomedicines 2023-06-15 [PMID: 37371820] (Human)
• Chen, L;Liu, Y;Wang, Z;Zhang, L;Xu, Y;Li, Y;Zhang, L;Wang, G;Yang, S;Xue, G; Mesenchymal stem cell-derived extracellular vesicles protect against abdominal aortic aneurysm formation by inhibiting NET-induced ferroptosis Experimental & molecular medicine 2023-05-01 [PMID: 37121969] (Human)
• M Blanter, M Cockx, L Wittebols, S Abouelasra, M De Bondt, N Berghmans, N Pörtner, L Vanbrabant, N Lorent, M Gouwy, M Boon, S Struyf Sputum from patients with primary ciliary dyskinesia contains high numbers of dysfunctional neutrophils and inhibits efferocytosis Respiratory Research, 2022-12-17;23(1):359. 2022-12-17 [PMID: 36528664] (Human)
• Thiago Henrique Caldeira Oliveira, Vincent Vanheule, Sofie Vandendriessche, Fariba Poosti, Mauro Martins Teixeira, Paul Proost et al. The GAG-Binding Peptide MIG30 Protects against Liver Ischemia-Reperfusion in Mice International Journal of Molecular Sciences 2022-08-26 [PMID: 36077113] (Human)
• L Morimont, M Dechamps, C David, C Bouvy, C Gillot, H Haguet, J Favresse, L Ronvaux, J Candiracci, M Herzog, PF Laterre, J De Poorter, S Horman, C Beauloye, J Douxfils NETosis and Nucleosome Biomarkers in Septic Shock and Critical COVID-19 Patients: An Observational Study Biomolecules, 2022-07-27;12(8):. 2022-07-27 [PMID: 36008932] (Human)
• SA Maskarinec, M McKelvy, K Boyle, H Hotchkiss, ME Duarte, B Addison, N Amato, S Khandelwal, GM Arepally, GM Lee Neutrophil functional heterogeneity is a fixed phenotype and is associated with distinct gene expression profiles Journal of leukocyte biology, 2022-08-02;0(0):. 2022-08-02 [PMID: 35916035] (Human)
• MA Wesdorp, A Schwab, EI Bektas, R Narcisi, D Eglin, MJ Stoddart, GJVM Van Osch, M D'Este A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro Oncogene, 2022-07-02;20(0):627-637. 2022-07-02 [PMID: 35846845] (Human)
• VB Pacheco, G Nery, LL Fernandes, TV Santana, M Jimenez, L Borges, E Hatanaka, P Braga, FR Monteiro, JB Amaral, LS Alves, ALL Bachi, SG da Silva, A Birbrair, D Heller Salivary Proteome, Inflammatory, and NETosis Biomarkers in Older Adult Practitioners and Nonpractitioners of Physical Exercise Oxidative Medicine and Cellular Longevity, 2022-04-23;2022(0):3725056. 2022-04-23 [PMID: 35502212] (Human)
• KC Ngamsri, RA Putri, C Jans, K Schindler, A Fuhr, Y Zhang, J Gamper-Tsi, S Ehnert, FM Konrad CXCR4 and CXCR7 Inhibition Ameliorates the Formation of Platelet-Neutrophil Complexes and Neutrophil Extracellular Traps through Adora2b Signaling International Journal of Molecular Sciences, 2021-12-17;22(24):. 2021-12-17 [PMID: 34948374] (Human)
• S Cambier, M Metzemaeke, AC Carvalho, A Nooyens, C Jacobs, L Vanderbeke, B Malengier-, M Gouwy, E Heylen, P Meersseman, G Hermans, E Wauters, A Wilmer, C Consortium, D Schols, P Matthys, G Opdenakker, RE Marques, J Wauters, J Vandooren, P Proost Atypical response to bacterial co-infection and persistent neutrophilic broncho-alveolar inflammation distinguish critical COVID-19 from influenza JCI Insight, 2022-01-11;0(0):. 2022-01-11 [PMID: 34793331] (Human)
• J Huckriede, SB Anderberg, A Morales, F de Vries, M Hultström, A Bergqvist, JT Ortiz-Pére, JW Sels, K Wichapong, M Lipcsey, M van de Pol, A Larsson, T Luther, C Reutelings, PG de Frutos, R Frithiof, GAF Nicolaes Evolution of NETosis markers and DAMPs have prognostic value in critically ill COVID-19 patients Scientific Reports, 2021-08-03;11(1):15701. 2021-08-03 [PMID: 34344929] (Human)
• C Thomas, H Nothaft, R Yadav, C Fodor, A Alemka, O Oni, M Bell, B Rada, CM Szymanski Characterization of ecotin homologs from Campylobacter rectus and Campylobacter showae PLoS ONE, 2020-12-30;15(12):e0244031. 2020-12-30 [PMID: 33378351] (Human)
• SJCFM Moorlag, YA Rodriguez-, J Gillard, S Fanucchi, K Theunissen, B Novakovic, CM de Bont, Y Negishi, ET Fok, L Kalafati, P Verginis, VP Mourits, VACM Koeken, LCJ de Bree, GJM Pruijn, C Fenwick, R van Crevel, LAB Joosten, I Joosten, H Koenen, MM Mhlanga, DA Diavatopou, T Chavakis, MG Netea BCG Vaccination Induces Long-Term Functional Reprogramming of Human Neutrophils Cell Rep, 2020-11-17;33(7):108387. 2020-11-17 [PMID: 33207187] (Human)
• J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants Bone Rep, 2020-09-11;13(0):100715. 2020-09-11 [PMID: 32995386] (Human)
• S Castellani, S D'Oria, A Diana, AM Polizzi, S Di Gioia, MA Mariggiò, L Guerra, M Favia, A Vinella, G Leonetti, D De Venuto, C Gallo, P Montemurro, M Conese G-CSF and GM-CSF Modify Neutrophil Functions at Concentrations found in Cystic Fibrosis Sci Rep, 2019-09-10;9(1):12937. 2019-09-10 [PMID: 31506515] (Human)
• W Hao, M Li, Y Zhang, C Zhang, Y Xue Expressions of MMP-12, TIMP-4, and Neutrophil Elastase in PBMCs and Exhaled Breath Condensate in Patients with COPD and Their Relationships with Disease Severity and Acute Exacerbations J Immunol Res, 2019-04-17;2019(0):7142438. 2019-04-17 [PMID: 31143784] (Human)
• HM Alyami, LS Finoti, HS Teixeira, A Aljefri, DF Kinane, MR Benakanake Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis Microb. Pathog., 2019-03-30;0(0):. 2019-03-30 [PMID: 30940608] (Human)

FAQs for Neutrophil Elastase/ELA2 (DY9167-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.