Human Myeloperoxidase DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Myeloperoxidase DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MPO

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Myeloperoxidase DuoSet ELISA, 15 Plate

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.

Publications for Myeloperoxidase/MPO (DY3174)(31)

Publications using DY3174

• Da Silva Cunha, AL;Blanter, M;Renders, J;Gouwy, M;Lorent, N;Boon, M;Struyf, S;Carlon, MS; Inhibiting CFTR through inh-172 in primary neutrophils reveals CFTR-specific functional defects Scientific reports 2024-12-28 [PMID: 39732786] (Human)
• Mirandola, A;Kudriavtsev, A;Cofre Muñoz, CI;Navarro, RC;Macagno, M;Daoud, S;Sanchez, C;Pastor, B;Pisareva, E;Marin, MS;Ruiz, JG;Piris, A;Rodriguez, AG;Gonzalez, NS;Vivancos, A;Quarà, V;Mellano, A;Borghi, F;Corti, G;Marchiò, C;Sapino, A;Bartolini, A;Crisafulli, G;Bardelli, A;Di Maio, M;Lossaint, G;Frayssinoux, F;Crapez, E;Ychou, M;Soler, RS;Fenocchio, E;Fernandez Calotti, PX;Mazard, T;Vivas, CS;Elez, E;Di Nicolantonio, F;Thierry, AR; Post-surgery sequelae unrelated to disease progression and chemotherapy revealed in follow-up of patients with stage III colon cancer EBioMedicine 2024-09-19 [PMID: 39303668] (Human)
• Cabrera, LE;Jokiranta, ST;Mäki, S;Miettinen, S;Kant, R;Kareinen, L;Sironen, T;Pietilä, JP;Kantele, A;Kekäläinen, E;Lindgren, H;Mattila, P;Kipar, A;Vapalahti, O;Strandin, T; The assembly of neutrophil inflammasomes during COVID-19 is mediated by type I interferons PLoS pathogens 2024-08-01 [PMID: 39172744] (Human)
• Nicola, T;Wenger, N;Xu, X;Evans, M;Qiao, L;Rezonzew, G;Yang, Y;Jilling, T;Margaroli, C;Genschmer, K;Willis, K;Ambalavanan, N;Blalock, JE;Gaggar, A;Lal, CV; A lactobacilli-based inhaled live biotherapeutic product attenuates pulmonary neutrophilic inflammation Nature communications 2024-08-19 [PMID: 39160214] (Human)
• Grannes, H;Ueland, T;Simeone, P;Liani, R;Guagnano, MT;Aukrust, P;Michelsen, AE;Birkeland, K;di Castelnuovo, A;Cipollone, F;Consoli, A;Halvorsen, B;Gregersen, I;Santilli, F; Liraglutide and not lifestyle intervention reduces soluble CD163 after comparable weight loss in obese participants with prediabetes or type 2 diabetes mellitus Cardiovascular diabetology 2024-04-29 [PMID: 38685051] (Human)
• Vozza, EG;Daly, CM;O'Rourke, SA;Fitzgerald, HK;Dunne, A;McLoughlin, RM; Staphylococcus aureus suppresses the pentose phosphate pathway in human neutrophils via the adenosine receptor A2aR to enhance intracellular survival mBio 2023-12-18 [PMID: 38108639] (Human)
• Mihalic, ZN;Kloimböck, T;Cosic-Mujkanovic, N;Valadez-Cosmes, P;Maitz, K;Kindler, O;Wadsack, C;Heinemann, A;Marsche, G;Gauster, M;Pollheimer, J;Kargl, J; Myeloperoxidase enhances the migration and invasion of human choriocarcinoma JEG-3 cells Redox biology 2023-09-19 [PMID: 37776707] (Human)
• Flockhart, M;Nilsson, LC;Tillqvist, EN;Vinge, F;Millbert, F;Lännerström, J;Nilsson, PH;Samyn, D;Apró, W;Sundqvist, ML;Larsen, FJ; Glucosinolate-rich broccoli sprouts protect against oxidative stress and improve adaptations to intense exercise training Redox biology 2023-09-03 [PMID: 37688976] (Human)
• Pastorek, M;Kone?ná, B;Janko, J;Janovi?ová, ?;Podracká, ?;Záhumenský, J;Šte?ová, E;Dúbrava, M;Hodosy, J;Vlková, B;Celec, P; Mitochondria-induced formation of neutrophil extracellular traps is enhanced in the elderly via Toll-like receptor 9 Journal of leukocyte biology 2023-08-30 [PMID: 37648664] (Human)
• A Gaál Koval, ? Janovi?ová, J Hodosy, J Bábí?ková, D Vavrincová, P Vavrinec, P Boor, ? Podracká, K Šebeková, P Celec, ? Tóthová Extracellular DNA concentrations in various aetiologies of acute kidney injury Scientific Reports, 2022-10-07;12(1):16812. 2022-10-07 [PMID: 36207374] (Human)
• AR Gründing, MA Schneider, S Richtmann, M Kriegsmann, H Winter, B Martinez-D, S Varona, B Liu, DS DeLuca, J Held, S Wrenger, T Muley, M Meister, T Welte, S Janciauski Lung Adenocarcinoma Cell Sensitivity to Chemotherapies: A Spotlight on Lipid Droplets and SREBF1 Gene Cancers, 2022-09-14;14(18):. 2022-09-14 [PMID: 36139614] (Human)
• Thiago Henrique Caldeira Oliveira, Vincent Vanheule, Sofie Vandendriessche, Fariba Poosti, Mauro Martins Teixeira, Paul Proost et al. The GAG-Binding Peptide MIG30 Protects against Liver Ischemia-Reperfusion in Mice International Journal of Molecular Sciences 2022-08-26 [PMID: 36077113] (Human)
• K Nakamura, H Nakayama, S Sasaki, K Takahashi, K Iwabuchi Mycobacterium avium-intracellulare complex promote release of pro-inflammatory enzymes matrix metalloproteinases by inducing neutrophil extracellular trap formation Scientific Reports, 2022-04-11;12(1):5181. 2022-04-11 [PMID: 35410994] (Human)
• S Hakki, EJ Robinson, MG Robson Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula Scientific Reports, 2022-01-26;12(1):1427. 2022-01-26 [PMID: 35082332] (Human)
• A Topf, M Mirna, N Bacher, V Paar, C Edlinger, LJ Motloch, S Gharibeh, M Bannehr, UC Hoppe, M Lichtenaue The Value of Fetuin-A as a Predictor to Identify Takotsubo Patients at Risk of Cardiovascular Events Journal of cardiovascular development and disease, 2021-10-05;8(10):. 2021-10-05 [PMID: 34677196] (Human)
• LE Cabrera, PT Pekkarinen, M Alander, KHA Nowlan, NA Nguyen, S Jokiranta, S Kuivanen, A Patjas, S Mero, SH Pakkanen, S Heinonen, A Kantele, O Vapalahti, E Kekäläinen, T Strandin Characterization of low-density granulocytes in COVID-19 PloS Pathogens, 2021-07-06;17(7):e1009721. 2021-07-06 [PMID: 34228753] (Human)
• GR Kathamuthu, K Moideen, R Sridhar, D Baskaran, S Babu Reduced neutrophil granular proteins and post-treatment modulation in tuberculous lymphadenitis PLoS ONE, 2021-06-21;16(6):e0253534. 2021-06-21 [PMID: 34153068] (Human)
• JH Jeong, SJ Choi, SM Ahn, JS Oh, YG Kim, CK Lee, B Yoo, S Hong Neutrophil extracellular trap clearance by synovial macrophages in gout Arthritis Research & Therapy, 2021-03-19;23(1):88. 2021-03-19 [PMID: 33741037] (Human)
• M Tong, SE Hanna, VM Abrahams Polymicrobial stimulation of human fetal membranes induce neutrophil activation and neutrophil extracellular trap release Journal of reproductive immunology, 2021-03-09;145(0):103306. 2021-03-09 [PMID: 33725528] (Human)
• A Minami-Tak, H Iwata, K Miyosawa, T Shiozawa, H Hayashi, T Funamizu, K Ishii, Y Nozaki, H Tabuchi, G Sekita, K Shimada, M Sumiyoshi, Y Nakazato, H Daida, T Minamino The association between impairment of HDL cholesterol efflux capacity and atrial remodeling in atrial fibrillation Scientific Reports, 2021-02-11;11(1):3547. 2021-02-11 [PMID: 33574408] (Human)
• E Nikolaou, SP Jochems, E Mitsi, S Pojar, A Blizard, J Reiné, C Solórzano, E Negera, B Carniel, A Soares-Sch, V Connor, H Adler, SR Zaidi, C Hales, H Hill, A Hyder-Wrig, SB Gordon, J Rylance, DM Ferreira Experimental Human Challenge Defines Distinct Pneumococcal Kinetic Profiles and Mucosal Responses between Colonized and Non-Colonized Adults MBio, 2021-01-12;12(1):. 2021-01-12 [PMID: 33436429] (Human)
• C Thomas, H Nothaft, R Yadav, C Fodor, A Alemka, O Oni, M Bell, B Rada, CM Szymanski Characterization of ecotin homologs from Campylobacter rectus and Campylobacter showae PLoS ONE, 2020-12-30;15(12):e0244031. 2020-12-30 [PMID: 33378351] (Human)
• J Theprungsi, JT Thaden, RM Wierzbicki, AS Burns, S Skopelja-G, VG Fowler, KL Winthrop, IW Martin, WFC Rigby Low-Avidity Autoantibodies Against Bactericidal/Permeability Increasing Protein Occur in Gram-Negative and Gram-Positive Bacteremia Infect. Immun., 2020-09-18;0(0):. 2020-09-18 [PMID: 32747603] (Human)
• RD Williamson, FP McCarthy, LC Kenny, CM McCarthy Activation of a TLR9 mediated innate immune response in preeclampsia Sci Rep, 2019-04-11;9(1):5920. 2019-04-11 [PMID: 30976066] (Human)
• HM Alyami, LS Finoti, HS Teixeira, A Aljefri, DF Kinane, MR Benakanake Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis Microb. Pathog., 2019-03-30;0(0):. 2019-03-30 [PMID: 30940608] (Human)
• D Emal, E Rampanelli, N Claessen, FJ Bemelman, JC Leemans, S Florquin, MC Dessing Calcineurin inhibitor Tacrolimus impairs host immune response against urinary tract infection Sci Rep, 2019-01-14;9(1):106. 2019-01-14 [PMID: 30643171] (Human)
• T Barliya, R Dardik, Y Nisgav, M Dachbash, D Gaton, G Kenet, R Ehrlich, D Weinberger, T Livnat Possible involvement of NETosis in inflammatory processes in the eye: Evidence from a small cohort of patients Mol. Vis., 2017-12-13;23(0):922-932. 2017-12-13 [PMID: 29296072] (Human)
• L Rocha-Penh, M Caldeira-D, JE Tanus-Sant, R de Carvalh, VC Sandrim Myeloperoxidase in Hypertensive Disorders of Pregnancy and Its Relation With Nitric Oxide Hypertension, 2017-05-01;0(0):. 2017-05-01 [PMID: 28461600] (Human)
• Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa PLoS Pathog., 2016-11-17;12(11):e1005987. 2016-11-17 [PMID: 27855208] (Human)
• Koepke J, Dresel M, Schmid S, Greulich T, Beutel B, Schmeck B, Vogelmeier C, Janciauskiene S, Koczulla A Therapy with plasma purified alpha1-antitrypsin (Prolastin(R)) induces time-dependent changes in plasma levels of MMP-9 and MPO. PLoS ONE, 2015-01-30;10(0):. 2015-01-30 [PMID: 25635861] (Human)
• Yoo D, Winn M, Pang L, Moskowitz S, Malech H, Leto T, Rada B Release of cystic fibrosis airway inflammatory markers from Pseudomonas aeruginosa-stimulated human neutrophils involves NADPH oxidase-dependent extracellular DNA trap formation. J Immunol, 2014-04-16;192(10):4728-38. 2014-04-16 [PMID: 24740504] (Human)

FAQs for Myeloperoxidase/MPO (DY3174). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: MPO