Human MIF Quantikine ELISA Kit



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Reactivity HuSpecies Glossary
Applications ELISA

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Human MIF Quantikine ELISA Kit Summary

The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard cur...ves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.
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Natural and recombinant human MIF
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
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Application Notes
No significant interference observed with available related molecules.
Read Publications using DMF00B.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human MIF Quantikine ELISA Kit

  • EC
  • EC
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase


MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for MIF (DMF00B)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using DMF00B Applications Species
HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth Cells, 2020;9(6):. 2020 [PMID: 32481584] (Human) Human
J Ning, L Xu, CQ Shen, YY Zhang, Q Zhao Increased serum levels of macrophage migration inhibitory factor in autism spectrum disorders Neurotoxicology, 2018;71(0):1-5. 2018 [PMID: 30503813] (Human) Human
M Pawlitzki, CM Sweeney-Re, SG Meuth, D Reinhold, J Neumann CSF macrophage migration inhibitory factor levels did not predict steroid treatment response after optic neuritis in patients with multiple sclerosis PLoS ONE, 2018;13(11):e0207726. 2018 [PMID: 30475854] (Human) Human
L Di Serafin, J Bartunek, G Heyndrickx, K Dierickx, G Scognamigl, T Tesorio, B De Bruyne, B Trimarco, W Wijns, E Barbato Macrophage migration inhibitory factor (MIF) is associated with degree of collateralization in patients with totally occluded coronary arteries Int. J. Cardiol., 2018;0(0):. 2018 [PMID: 29602581] (Human) Human
S Okazaki, A Hishimoto, I Otsuka, Y Watanabe, S Numata, S Boku, N Shimmyo, M Kinoshita, E Inoue, T Ohmori, T Someya, I Sora Increased serum levels and promoter polymorphisms of macrophage migration inhibitory factor in schizophrenia Prog. Neuropsychopharmacol. Biol. Psychiatry, 2018;83(0):33-41. 2018 [PMID: 29305329] (Human) Human
M Zimmerman, S Rolandsson, H Skärstrand, K Pourhamidi, A Gottsäter, P Wollmer, O Rolandsson, G Westergren, LB Dahlin Temporal trend of autonomic nerve function and HSP27, MIF and PAI-1 in type 1 diabetes J Clin Transl Endocrinol, 2017;8(0):15-21. 2017 [PMID: 29067254] (Human) Human
G Benedek, R Meza-Romer, K Jordan, Y Zhang, H Nguyen, G Kent, J Li, E Siu, J Frazer, M Piecychna, X Du, A Sreih, L Leng, J Wiedrick, SJ Caillier, H Offner, JR Oksenberg, V Yadav, D Bourdette, R Bucala, AA Vandenbark MIF and D-DT are potential disease severity modifiers in male MS subjects Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28923927] (Human) Human
J Baron-Stef, J Schiefer, EJ Miller, GA Berlakovic, DM Baron, P Faybik Comparison of macrophage migration inhibitory factor and neutrophil gelatinase-associated lipocalin-2 to predict acute kidney injury after liver transplantation: An observational pilot study PLoS ONE, 2017;12(8):e0183162. 2017 [PMID: 28813470] (Human) Human
R Kumar, T de Mooij, TE Peterson, T Kaptzan, AJ Johnson, DJ Daniels, IF Parney Modulating glioma-mediated myeloid-derived suppressor cell development with sulforaphane PLoS ONE, 2017;12(6):e0179012. 2017 [PMID: 28666020] (Human) Human
S Grygorczuk, M Parczewski, R ?wierzbi?s, P Czupryna, A Moniuszko, J Dunaj, M Kondrusik, S Pancewicz The increased concentration of macrophage migration inhibitory factor in serum and cerebrospinal fluid of patients with tick-borne encephalitis J Neuroinflammation, 2017;14(1):126. 2017 [PMID: 28646884] (Human) Human
Show All 25 Publications.

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FAQs for MIF (DMF00B). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Gene Symbol MIF