Human MIF Quantikine ELISA Kit



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Reactivity HuSpecies Glossary
Applications ELISA

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Human MIF Quantikine ELISA Kit Summary

The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard cur...ves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.
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Natural and recombinant human MIF
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Spike Recovery
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Application Notes
No significant interference observed with available related molecules.
Read Publications using DMF00B.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human MIF Quantikine ELISA Kit

  • EC
  • EC
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase


MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for MIF (DMF00B)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using DMF00B Applications Species
Wu, L;Gao, Y;Xie, S;Ye, W;Uemura, Y;Zhang, R;Yu, Y;Li, J;Chen, M;Wu, Q;Cui, P;Liu, H;Mu, S;Li, Y;Wang, L;Liu, C;Li, J;Zhang, L;Jiao, S;Zhang, G;Liu, T; The level of macrophage migration inhibitory factor is negatively correlated with the efficacy of PD-1 blockade immunotherapy combined with chemotherapy as a neoadjuvant therapy for esophageal squamous cell carcinoma Translational oncology 2023-09-05 [PMID: 37678132] (Human) Human
MS Simon, BB Barton, C Glocker, R Musil A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol PLoS ONE, 2022-07-21;17(7):e0271793. 2022-07-21 [PMID: 35862413] (Human) Human
L Garcia-Ger, M García, A Garrido-Ga, S Gómez-Gonz, M Torrebadel, E Prada, G Pascual-Pa, O Muñoz, S Perez-Jaum, I Lemos, N Salvador, M Vila-Ubach, A Doncel-Req, M Suñol, AM Carcaboso, J Mora, C Lavarino MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment BMC Cancer, 2022-06-17;22(1):669. 2022-06-17 [PMID: 35715791] (Human) Human
JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis Cell Death & Disease, 2022-06-06;13(6):527. 2022-06-06 [PMID: 35661105] (Human) Human
G Benedek, M Abed El La, K Miller, M Rivkin, A Ahmed Rama, L P Riek, R Lako, S Edvardson, S Arbel-Alon, E Galun, M Levite Macrophage migration inhibitory factor in Nodding syndrome PloS Neglected Tropical Diseases, 2021-10-18;15(10):e0009821. 2021-10-18 [PMID: 34662363] (Human) Human
KH Chong, MH Wu, CW Chen, TH Hsieh, CW Lai Preoperative Serum Macrophage Migration Inhibitory Factor Level Correlates with Surgical Difficulty and Outcome in Patients with Autoimmune Thyroiditis Journal of Clinical Medicine, 2021-09-07;10(18):. 2021-09-07 [PMID: 34575145] (Human) Human
HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth Cells, 2020-05-28;9(6):. 2020-05-28 [PMID: 32481584] (Human) Human
GL Du, JY Luo, D Wang, YH Li, BB Fang, XM Li, XM Gao, YN Yang MIF gene rs755622 polymorphism positively associated with acute coronary syndrome in Chinese Han population: case-control study Sci Rep, 2020-01-10;10(1):140. 2020-01-10 [PMID: 31924846] (Human) Human
J Ning, L Xu, CQ Shen, YY Zhang, Q Zhao Increased serum levels of macrophage migration inhibitory factor in autism spectrum disorders Neurotoxicology, 2018-11-29;71(0):1-5. 2018-11-29 [PMID: 30503813] (Human) Human
M Pawlitzki, CM Sweeney-Re, SG Meuth, D Reinhold, J Neumann CSF macrophage migration inhibitory factor levels did not predict steroid treatment response after optic neuritis in patients with multiple sclerosis PLoS ONE, 2018-11-26;13(11):e0207726. 2018-11-26 [PMID: 30475854] (Human) Human
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Gene Symbol MIF