Human Lipocalin-2/NGAL DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Lipocalin-2/NGAL DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: LCN2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Lipocalin-2/NGAL DuoSet ELISA, 15 Plate

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Publications for Lipocalin-2/NGAL (DY1757)(40)

Publications using DY1757

• Rivera, AO;Held, J;Beimdiek, J;Buettner, FFR;Heu beta el, CP;Janciauskiene, S;Trudzinski, F; Characterization of alpha-1 antitrypsin in a Pi?ZZ patient with emphysema: a case report Respiratory medicine case reports 2025-10-17 [PMID: 41216612] (Human)
• Somova, M;Simm, S;Ehrhardt, J;Schoon, J;Burchardt, M;Pinto, PC; SARS-CoV-2 Spike Protein Amplifies the Immunogenicity of Healthy Renal Epithelium in the Presence of Renal Cell Carcinoma Cells 2024-12-10 [PMID: 39768130] (ELISA, Human)
• Hanford, HE;Price, CTD;Uriarte, S;Abu Kwaik, Y; Inhibition and evasion of neutrophil microbicidal responses by Legionella longbeachae mBio 2024-12-16 [PMID: 39679679] (Human)
• Williams, ME;Asia, LK;Lindeque, Z;Jansen van Vuren, E; The association between HIV-1 Tat and Vif amino acid sequence variation, inflammation and Trp-Kyn metabolism: an exploratory investigation BMC infectious diseases 2024-09-09 [PMID: 39251983] (Human)
• Herfindal, AM;Nilsen, M;Aspholm, TE;Schultz, GIG;Valeur, J;Rudi, K;Thoresen, M;Lundin, KEA;Henriksen, C;Bøhn, SK; Effects of fructan and gluten on gut microbiota in individuals with self-reported non-celiac gluten/wheat sensitivity-a randomised controlled crossover trial BMC medicine 2024-09-04 [PMID: 39227818] (Human)
• Cho, HY;Lee, JE;Park, KH;Choi, BY;Lee, MJ;Jeong, DE;Shin, S; Identification and characterization of plasma proteins associated with intra-amniotic inflammation and/or infection in women with preterm labor. Scientific reports 2024-06-25 [PMID: 38918423] (Human)
• de Rooij, ENM;Hoogeveen, EK;Romijn, FPHTM;van der Kooy, SW;Veighey, KV;Dekker, FW;van Kooten, C;Cobbaert, CM;de Fijter, JW; Urinary beta-2 microglobulin increases whereas TIMP-2 and IGFBP7 decline after unilateral nephrectomy in healthy kidney donors Scientific reports 2024-06-05 [PMID: 38839764] (Human)
• Xu, S;Hultström, M;Larsson, A;Lipcsey, M;Lindskog, C;Bülow, S;Frithiof, R;Venge, P; The Human Phospholipase B-II Precursor (HPLBII-P) in Urine as a Novel Biomarker of Glomerular Activity in COVID-19 and Diabetes Mellitus Journal of clinical medicine 2024-04-26 [PMID: 38731071] (Human)
• Grannes, H;Ueland, T;Simeone, P;Liani, R;Guagnano, MT;Aukrust, P;Michelsen, AE;Birkeland, K;di Castelnuovo, A;Cipollone, F;Consoli, A;Halvorsen, B;Gregersen, I;Santilli, F; Liraglutide and not lifestyle intervention reduces soluble CD163 after comparable weight loss in obese participants with prediabetes or type 2 diabetes mellitus Cardiovascular diabetology 2024-04-29 [PMID: 38685051] (Human)
• Nagayama, I;Takayanagi, K;Nagata, D;Hasegawa, H;Maeshima, A; Potential Utility of Urinary Follistatin as a Non-Invasive Indicator of Acute Tubular Damage in Patients with Acute Kidney Injury Cells 2024-03-16 [PMID: 38534369] (Human)
• Ramakrishnan, G;Young, MK;Nayak, U;Rigo, I;Marrs, AS;Gilchrist, CA;Behm, BW;Madden, GR;Petri, WA; Systemic neutrophil degranulation and emergency granulopoiesis in patients with Clostridioides difficile infection Anaerobe 2024-03-19 [PMID: 38514010] (Human)
• Paparella, R;Ferraguti, G;Fiore, M;Menghi, M;Micangeli, G;Tarani, F;Ligotino, A;Messina, MP;Ceccanti, M;Minni, A;Barbato, C;Lucarelli, M;Tarani, L;Petrella, C; Serum Lipocalin-2 Levels as a Biomarker in Pre- and Post-Pubertal Klinefelter Syndrome Patients: A Pilot Study International journal of molecular sciences 2024-02-12 [PMID: 38396890] (Human)
• Lablad, Y;Vanhomwegen, C;De Prez, E;Antoine, MH;Hasan, S;Baudoux, T;Nortier, J; Longitudinal Follow-Up of Serum and Urine Biomarkers Indicative of COVID-19-Associated Acute Kidney Injury: Diagnostic and Prognostic Impacts International journal of molecular sciences 2023-11-18 [PMID: 38003685] (Human)
• Ravn, EJ;Hasific, S;Thomassen, M;Hjortebjerg, R;Bach Laursen, K;Diederichsen, A;Bistrup, C;Øvrehus, KA; Intravenous versus oral hydration to reduce the risk of postcontrast acute kidney injury after intravenous contrast-enhanced CT in patients with severe chronic kidney disease (ENRICH): a study protocol for a single-centre, parallel-group, open-labelled non-inferiority randomised controlled trial in Denmark BMJ open 2023-09-12 [PMID: 37699636] (Human)
• Goulet, N;Tetzlaff, EJ;Morin, R;Mauger, JF;Amaratunga, R;Kenny, GP;Imbeault, P; No impact of a high-fat meal coupled with intermittent hypoxemia on acute kidney injury biomarkers in adults with and without obstructive sleep apnea Physiological reports 2023-09-01 [PMID: 37653582] (Human)
• Z Cao, IJ Burvenich, K Zhao, C Senko, J Glab, R Fogliaro, Z Liu, I Jose, H Puthalakat, NJ Hoogenraad, LD Osellame, AM Scott Identification of Potential Biomarkers for Cancer Cachexia and Anti-Fn14 Therapy Cancers, 2022-11-10;14(22):. 2022-11-10 [PMID: 36428623] (Human)
• PJW Naudé, C Pariante, N Hoffman, SM Koopowitz, KA Donald, HJ Zar, DJ Stein Antenatal maternal depression, early life inflammation and neurodevelopment in a South African birth cohort Brain, Behavior, and Immunity, 2022-07-06;105(0):160-168. 2022-07-06 [PMID: 35803482] (Human)
• HM Demerdash, AA Sabry, OE Arida Impact of Zinc to Copper Ratio and Lipocalin 2 in Obese Patients Undergoing Sleeve Gastrectomy Oncogene, 2022-06-10;2022(0):9278531. 2022-06-10 [PMID: 35722455] (Human)
• S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department Scientific Reports, 2022-05-24;12(1):8733. 2022-05-24 [PMID: 35610344] (Human)
• JH Kim, H Lee, J Oh, K Suk, BY Chun Increased Plasma Lipocalin-2 Levels in Patients with Myelin Oligodendrocyte Glycoprotein-IgG-Positive Optic Neuritis Journal of Clinical Medicine, 2022-05-07;11(9):. 2022-05-07 [PMID: 35566760] (Human)
• M Lipcsey, M Bergquist, R Sirén, A Larsson, F Huss, J Pravda, M Furebring, J Sjölin, H Janols Urine Hydrogen Peroxide Levels and Their Relation to Outcome in Patients with Sepsis, Septic Shock, and Major Burn Injury Biomedicines, 2022-04-05;10(4):. 2022-04-05 [PMID: 35453598] (Human)
• ACC Loureiro, GF Nocrato, ALL Correia, RS de Matos, JCCN Filho, EF Daher, FHM Pinto, AC de Oliveir, VM Ceccatto, RS Fortunato, DP de Carvalh Serum and Urinary Neutrophil Gelatinase-Associated Lipocalin Are Not Associated With Serum Redox Parameters in Amateur Athletes After an Ultramarathon Frontiers in Physiology, 2022-03-17;13(0):811514. 2022-03-17 [PMID: 35370771] (Human)
• S Hong, KH Park, YE Lee, JE Lee, YM Kim, E Joo, I Cho Antibody microarray analysis of amniotic fluid proteomes in women with cervical insufficiency and short cervix, and their association with pregnancy latency length PLoS ONE, 2022-02-07;17(2):e0263586. 2022-02-07 [PMID: 35130326] (Human)
• Ingunn Bakke, Gunnar Andreas Walaas, Torunn Bruland, Elin Synnøve Røyset, Atle van Beelen Granlund, Celia Escudero-Hernández et al. Mucosal and faecal neutrophil gelatinase-associated lipocalin as potential biomarkers for collagenous colitis Journal of Gastroenterology 2021-10-01 [PMID: 34414506]
• A Gradin, H Andersson, T Luther, SB Anderberg, S Rubertsson, M Lipcsey, M Åberg, A Larsson, R Frithiof, M Hultström Urinary cytokines correlate with acute kidney injury in critically ill COVID-19 patients Cytokine, 2021-05-24;146(0):155589. 2021-05-24 [PMID: 34161857] (Human)
• B Olson, X Zhu, MA Norgard, PR Levasseur, JT Butler, A Buenafe, KG Burfeind, KA Michaelis, KR Pelz, H Mendez, J Edwards, SM Krasnow, AJ Grossberg, DL Marks Lipocalin 2 mediates appetite suppression during pancreatic cancer cachexia Nature Communications, 2021-04-06;12(1):2057. 2021-04-06 [PMID: 33824339] (Human)
• C Veraar, J Kliman, A Benazzo, F Oberndorfe, M Laggner, P Hacker, T Raunegger, S Janik, P Jaksch, W Klepetko, HJ Ankersmit, B Moser Potential novel biomarkers for chronic lung allograft dysfunction and azithromycin responsive allograft dysfunction Scientific Reports, 2021-03-24;11(1):6799. 2021-03-24 [PMID: 33762606] (Human)
• L Kang, S Smith, C Wang Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating Journal of colloid and interface science, 2020-12-24;588(0):101-109. 2020-12-24 [PMID: 33388576] (Human)
• HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients PLoS ONE, 2020-12-16;15(12):e0243759. 2020-12-16 [PMID: 33326471] (Human)
• M Bergquist, L Samuelsson, A Larsson, J Tydén, J Johansson, M Lipcsey TNFR1, TNFR2, neutrophil gelatinase-associated lipocalin and heparin binding protein in identifying sepsis and predicting outcome in an intensive care cohort Scientific Reports, 2020-09-18;10(1):15350. 2020-09-18 [PMID: 32948801] (Human)
• B Cuffaro, ALW Assohoun, D Boutillier, L Súkeníková, J Desramaut, S Boudebbouz, S Salomé-Des, J Hrdý, AJ Waligora-D, E Maguin, C Grangette In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice Cells, 2020-09-16;9(9):. 2020-09-16 [PMID: 32947881] (Human)
• A Stanton, C Mowbray, M Lanz, K Brown, P Hilton, A Tyson-Capp, RS Pickard, ASM Ali, J Hall Topical Estrogen Treatment Augments the Vaginal Response to Escherichia coli Flagellin Sci Rep, 2020-05-21;10(1):8473. 2020-05-21 [PMID: 32439855] (Human)
• J Merkle, A Daka, AC Deppe, T Wahlers, A Paunel-Gör High levels of cell-free DNA accurately predict late acute kidney injury in patients after cardiac surgery PLoS ONE, 2019-06-18;14(6):e0218548. 2019-06-18 [PMID: 31211810] (Human)
• YT Kang, WC Hsu, CH Wu, IL Hsin, PR Wu, KT Yeh, JL Ko Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells Oncotarget, 2017-11-06;8(62):105536-105552. 2017-11-06 [PMID: 29285270] (Human)
• Microbiota-inducible Innate Immune, Siderophore Binding Protein Lipocalin 2 is Critical for Intestinal Homeostasis Cell Mol Gastroenterol Hepatol, 2016-07-01;2(4):482-498.e6. 2016-07-01 [PMID: 27458605] (Human)
• Silk-based blood stabilization for diagnostics Proc Natl Acad Sci USA, 2016-05-09;113(21):5892-7. 2016-05-09 [PMID: 27162330] (Human)
• Zager R, Johnson A, Frostad K Rapid renal alpha-1 antitrypsin gene induction in experimental and clinical acute kidney injury. PLoS ONE, 2014-05-21;9(5):e98380. 2014-05-21 [PMID: 24848503] (Human)
• Zughaier , Susu M, Kandler , Justin L, Shafer , William Neisseria gonorrhoeae modulates iron-limiting innate immune defenses in macrophages. PLoS ONE, 2014-01-28;9(1):e87688. 2014-01-28 [PMID: 24489950] (Human)
• Paton C, Rogowski M, Kozimor A, Stevenson J, Chang H, Cooper J Lipocalin-2 increases fat oxidation in vitro and is correlated with energy expenditure in normal weight but not obese women. Obesity (Silver Spring), 2013-07-29;21(12):E640-8. 2013-07-29 [PMID: 23640923] (Human)
• Alvarez J, Zughaier S, Law J, Hao L, Wasse H, Ziegler T, Tangpricha V Effects of high-dose cholecalciferol on serum markers of inflammation and immunity in patients with early chronic kidney disease. Eur J Clin Nutr, 2013-01-30;67(3):264-9. 2013-01-30 [PMID: 23361158] (Human)

FAQs for Lipocalin-2/NGAL (DY1757). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LCN2