Human IL-7 Quantikine HS ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-7 Quantikine HS ELISA Kit Summary

• Background: The Quantikine HS Human IL-7 Immunoassay is a solid phase ELISA that specifically measures IL-7 in 18-24 hours (with an overnight incubation step). This kit is designed to measure IL-7 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-7 and antibodies raised against the recombinant factor. It has been shown to quantitate the rec...ombinant factor accurately. Results obtained using natural IL-7 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-7.
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• Specificity: Natural and recombinant human IL-7
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL7

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-7 Quantikine HS ELISA Kit

• IL7
• IL-7
• IL-7interleukin-7
• interleukin 7
• Lymphopoietin-1
• PBGF

Background

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.

Publications for IL-7 (HS750)(47)

Publications using HS750

• Park, S;Lee, SM;Pak, KC;Byun, MS;Choi, D;Lim, HS; Population Pharmacokinetic and Pharmacodynamic Modeling Analysis of rhIL-7-hyFc, a Hybrid Fc-Fused Long-Acting Interleukin-7, to Support Optimal Dosing Regimens in Patients with Solid Cancer Drug design, development and therapy 2025-10-17 [PMID: 41127361] (Human)
• Dolna-Michno, J;Kopi?ski, P;Przybylski, G;Wypasek, E;Szyma?ska, M;W?drowska, E;Miko?ajczyk, K;Senderek, T;Gagat, M; Cytoimmunological Profile of Lower Airways in Post-COVID-19 Syndrome (PCS): Predictive Value of Bronchoalveolar Lavage Journal of clinical medicine 2025-05-12 [PMID: 40429360] (Human)
• Chen, S;Nguyen, TD;Lee, KZ;Liu, D; Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies Biotechnology advances 2024-08-19 [PMID: 39168355] (Human)
• Y Kitamura, YI Koma, K Tanigawa, S Tsukamoto, Y Azumi, S Miyako, S Urakami, T Kodama, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Roles of IL-7R Induced by Interactions between Cancer Cells and Macrophages in the Progression of Esophageal Squamous Cell Carcinoma Cancers, 2023-01-06;15(2):. 2023-01-06 [PMID: 36672342] (Human)
• C Cuéllar, M Rodero, J Pérez-Grie, L Galindo-Re, F Lopez-Chul, C García-Bal, J Carlos And Association between anti-Anisakis simplex antibodies and interleukin-7 levels International immunopharmacology, 2022-08-11;111(0):109134. 2022-08-11 [PMID: 35964412] (Human)
• T Sainz, L Diaz, D Rojo, MI Clemente, C Barbas, MJ Gosalbes, N Jimenez-He, L Escosa, S Guillen, JT Ramos, MÁ Muñoz-Fern, ML Navarro, MJ Mellado, S Serrano-Vi Targeting the Gut Microbiota of Vertically HIV-Infected Children to Decrease Inflammation and Immunoactivation: A Pilot Clinical Trial Nutrients, 2022-02-26;14(5):. 2022-02-26 [PMID: 35267967] (Human)
• C Bacchus-So, M Fitch, J Symons, M Abdel-Mohs, DB Reeves, R Hoh, M Stone, J Hiatt, P Kim, A Chopra, H Ahn, VA York, DL Cameron, FM Hecht, JN Martin, SA Yukl, S Mallal, PU Cameron, SG Deeks, JT Schiffer, SR Lewin, MK Hellerstei, JM McCune, PW Hunt Relationship between CD4 T cell turnover, cellular differentiation and HIV persistence during ART PloS Pathogens, 2021-01-19;17(1):e1009214. 2021-01-19 [PMID: 33465157] (Human)
• J Xun, T Qi, L Zou, Q Tang, Y Shen, J Yang, L Xie, Y Ji, R Zhang, L Liu, J Wang, C Steinhart, Z Wang, Y Tang, W Song, J Sun, J Cheng, X Le, H Wu, X He, R Chen, J Chen, H Lu Mycobacterium tuberculosis co-infection is associated with increased surrogate marker of the HIV reservoir AIDS Res Ther, 2020-10-19;17(1):63. 2020-10-19 [PMID: 33076959] (Human)
• Y Yuan, C Zhou, Q Yang, S Ma, X Wang, X Guo, Y Ding, J Tang, Y Zeng, D Li HIV-1 Tat protein inhibits the hematopoietic support function of human bone marrow mesenchymal stem cells Virus Res., 2019-09-12;273(0):197756. 2019-09-12 [PMID: 31521762] (Human)
• A Rajamanick, S Munisankar, Y Bhootra, CK Dolla, K Thiruvenga, TB Nutman, S Babu Altered levels of memory T cell subsets and common ?c cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment PLoS Negl Trop Dis, 2018-05-24;12(5):e0006481. 2018-05-24 [PMID: 29795573] (Human)
• J Seyfarth, C Lundtoft, K Förtsch, H Ahlert, J Rosenbauer, C Baechle, M Roden, RW Holl, E Mayatepek, S Kummer, T Meissner, M Jacobsen IL7RA haplotypes differentially affect soluble IL-7 receptor and IL-7 serum concentrations in children with type 1 diabetes Pediatr Diabetes, 2018-03-13;0(0):. 2018-03-13 [PMID: 29484785] (Human)
• C Lundtoft, A Afum-Adjei, J Rimpler, K Harling, N Nausch, M Kohns, E Adankwah, F Lang, L Olbrich, E Mayatepek, E Owusu-Dabo, M Jacobsen Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis PLoS Pathog., 2017-06-05;13(6):e1006425. 2017-06-05 [PMID: 28582466] (Human)
• R Ponte, M Rancez, S Figueiredo, J Dutrieux, V Fabre-Mers, B Charmeteau, T Guilbert, JP Routy, R Cheynier, A Couëdel-Co Acute Simian Immunodeficiency Virus Infection Triggers Early and Transient Interleukin-7 Production in the Gut, Leading to Enhanced Local Chemokine Expression and Intestinal Immune Cell Homing Front Immunol, 2017-05-19;8(0):588. 2017-05-19 [PMID: 28579989] (Primate)
• NP Kumar, VV Banurekha, D Nair, S Babu Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment PLoS ONE, 2017-04-27;12(4):e0176495. 2017-04-27 [PMID: 28448542] (Human)
• P Severino, DT Palomino, H Alvarenga, CB Almeida, DC Pasqualim, A Cury, PR Salvalaggi, AL De Vasconc, MC Andrade, T Aloia, S Bromberg, LV Rizzo, FA Rocha, LC Marti Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli Front Immunol, 2017-02-14;8(0):141. 2017-02-14 [PMID: 28261205] (Human)
• Establishment of IL-7 Expression Reporter Human Cell Lines, and Their Feasibility for High-Throughput Screening of IL-7-Upregulating Chemicals PLoS ONE, 2016-09-02;11(9):e0161899. 2016-09-02 [PMID: 27589392] (Human)
• Okoye A, Rohankhedkar M, Konfe A, Abana C, Reyes M, Clock J, Duell D, Sylwester A, Sammader P, Legasse A, Park B, Axthelm M, Nikolich-Zugich J, Picker L Effect of IL-7 Therapy on Naive and Memory T Cell Homeostasis in Aged Rhesus Macaques. J Immunol, 2015-09-28;195(9):4292-305. 2015-09-28 [PMID: 26416281] (Primate - Macaca mulatta (Rhesus Macaque))
• Bazdar D, Kalinowska M, Panigrahi S, Sieg S Recycled IL-7 Can Be Delivered to Neighboring T Cells. J Immunol, 2015-04-13;194(10):4698-704. 2015-04-13 [PMID: 25870237] (Human)
• Hannon M, Beguin Y, Ehx G, Servais S, Seidel L, Graux C, Maertens J, Kerre T, Daulne C, De Bock M, Fillet M, Ory A, Willems E, Gothot A, Humblet-Baron S, Baron F Immune Recovery after Allogeneic Hematopoietic Stem Cell Transplantation Following Flu-TBI versus TLI-ATG Conditioning. Clin Cancer Res, 2015-03-16;21(14):3131-9. 2015-03-16 [PMID: 25779951] (Human)
• Bruel T, Hamimi C, Dereuddre-Bosquet N, Cosma A, Shin S, Corneau A, Versmisse P, Karlsson I, Malleret B, Targat B, Barre-Sinoussi F, Le Grand R, Pancino G, Saez-Cirion A, Vaslin B Long-term control of simian immunodeficiency virus (SIV) in cynomolgus macaques not associated with efficient SIV-specific CD8+ T-cell responses. J Virol, 2015-01-14;89(7):3542-56. 2015-01-14 [PMID: 25589645] (Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Kielsen K, Jordan K, Uhlving H, Pontoppidan P, Shamim Z, Ifversen M, Heilmann C, Nielsen C, Sengelov H, Ryder L, Muller K T cell reconstitution in allogeneic haematopoietic stem cell transplantation: prognostic significance of plasma interleukin-7. Scand J Immunol, 2015-01-01;81(1):72-80. 2015-01-01 [PMID: 25263171] (Human)
• Cha L, de Jong E, French M, Fernandez S IFN-alpha exerts opposing effects on activation-induced and IL-7-induced proliferation of T cells that may impair homeostatic maintenance of CD4+ T cell numbers in treated HIV infection. J Immunol, 2014-07-25;193(5):2178-86. 2014-07-25 [PMID: 25063872] (Human)
• Le Saout C, Hasley R, Imamichi H, Tcheung L, Hu Z, Luckey M, Park J, Durum S, Smith M, Rupert A, Sneller M, Lane H, Catalfamo M Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis. PLoS Pathog, 2014-03-06;10(3):e1003976. 2014-03-06 [PMID: 24603698] (Human)
• Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature. PLoS ONE, 2013-12-05;8(12):e81983. 2013-12-05 [PMID: 24349168] (Human)
• Cai , Yu-Jiao, Wang , Wen-Shen, Yang , Yang, Sun , Li-Hua, Teitelbaum , Daniel H, Yang , Hua Up-regulation of intestinal epithelial cell derived IL-7 expression by keratinocyte growth factor through STAT1/IRF-1, IRF-2 pathway. PLoS ONE, 2013-03-12;8(3):e58647. 2013-03-12 [PMID: 23554911] (Human)
• Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J The systemic cytokine environment is permanently altered in multiple myeloma. PLoS ONE, 2013-03-27;8(3):e58504. 2013-03-27 [PMID: 23544044] (Human)
• Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients. Genes Immun, 2012-08-23;13(7):587-92. 2012-08-23 [PMID: 22914435] (Human)
• Vassena L, Miao H, Cimbro R, Malnati MS, Cassina G, Proschan MA, Hirsch VM, Lafont BA, Morre M, Fauci AS, Lusso P Treatment with IL-7 prevents the decline of circulating CD4+ T cells during the acute phase of SIV infection in rhesus macaques. PLoS Pathog., 2012-04-12;8(4):e1002636. 2012-04-12 [PMID: 22511868] (Primate - Macaca mulatta (Rhesus Macaque))
• Steel C, Nutman TB Altered T cell memory and effector cell development in chronic lymphatic filarial infection that is independent of persistent parasite antigen. PLoS ONE, 2011-04-29;6(4):e19197. 2011-04-29 [PMID: 21559422] (Human)
• Thiant S, Labalette M, Trauet J, Coiteux V, de Berranger E, Dessaint JP, Yakoub-Agha I Plasma levels of IL-7 and IL-15 after reduced intensity conditioned allo-SCT and relationship to acute GVHD. Bone Marrow Transplant., 2010-12-06;46(10):1374-81. 2010-12-06 [PMID: 21132028] (Human)
• Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma. Clin. Exp. Immunol., 2010-10-05;162(3):447-59. 2010-10-05 [PMID: 20942806] (Human)
• Antonelli LR, Mahnke Y, Hodge JN, Porter BO, Barber DL, DerSimonian R, Greenwald JH, Roby G, Mican J, Sher A, Roederer M, Sereti I Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome. Blood, 2010-07-26;116(19):3818-27. 2010-07-26 [PMID: 20660788] (Human)
• Sugalski JM, Rodriguez B, Moir S Peripheral blood B cell subset skewing is associated with altered cell cycling and intrinsic resistance to apoptosis and reflects a state of immune activation in chronic hepatitis C virus infection. J. Immunol., 2010-07-23;185(5):3019-27. 2010-07-23 [PMID: 20656924] (Human)
• Thiant S, Yakoub-Agha I, Magro L, Trauet J, Coiteux V, Jouet JP, Dessaint JP, Labalette M Plasma levels of IL-7 and IL-15 in the first month after myeloablative BMT are predictive biomarkers of both acute GVHD and relapse. Bone Marrow Transplant., 2010-03-01;45(10):1546-52. 2010-03-01 [PMID: 20190846] (Human)
• Juffroy O, Bugault F, Lambotte O, Landires I, Viard JP, Niel L, Fontanet A, Delfraissy JF, Theze J, Chakrabarti LA Dual mechanism of impairment of interleukin-7 (IL-7) responses in human immunodeficiency virus infection: decreased IL-7 binding and abnormal activation of the JAK/STAT5 pathway. J. Virol., 2010-01-01;84(1):96-108. 2010-01-01 [PMID: 19864382] (Human)
• Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients. J. Immunol., 2009-06-15;182(12):7389-97. 2009-06-15 [PMID: 19494261] (Human)
• Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma. Int. J. Cancer, 2009-09-01;125(5):1092-101. 2009-09-01 [PMID: 19391137] (Human)
• Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D Identification of interleukin-7 as a candidate disease mediator in spondylarthritis. Arthritis Rheum., 2008-11-01;58(11):3430-5. 2008-11-01 [PMID: 18975340] (Human)
• Louis CU, Straathof K, Bollard CM, Gerken C, Huls MH, Gresik MV, Wu MF, Weiss HL, Gee AP, Brenner MK, Rooney CM, Heslop HE, Gottschalk S Enhancing the in vivo expansion of adoptively transferred EBV-specific CTL with lymphodepleting CD45 monoclonal antibodies in NPC patients. Blood, 2008-10-29;113(11):2442-50. 2008-10-29 [PMID: 18971421] (Human)
• Mancebo E, Clemente J, Sanchez J, Ruiz-Contreras J, De Pablos P, Cortezon S, Romo E, Paz-Artal E, Allende LM Longitudinal analysis of immune function in the first 3 years of life in thymectomized neonates during cardiac surgery. Clin. Exp. Immunol., 2008-09-22;154(3):375-83. 2008-09-22 [PMID: 18811694] (Human)
• Benedict C, Dimitrov S, Marshall L, Born J Sleep enhances serum interleukin-7 concentrations in humans. Brain Behav. Immun., 2007-05-23;21(8):1058-62. 2007-05-23 [PMID: 17524612] (Human)
• Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection. J. Immunol., 2007-04-15;178(8):5340-50. 2007-04-15 [PMID: 17404319] (Human)
• Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis. J. Pathol., 2007-05-01;212(1):47-55. 2007-05-01 [PMID: 17370327] (Human)
• Koesters SA, Alimonti JB, Wachihi C, Matu L, Anzala O, Kimani J, Embree JE, Plummer FA, Fowke KR IL-7Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation. Eur. J. Immunol., 2006-02-01;36(2):336-44. 2006-02-01 [PMID: 16421946] (Human)
• Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J. Immunol., 2005-12-01;175(11):7325-31. 2005-12-01 [PMID: 16301638] (Human)
• Muthukumar A, Wozniakowski A, Gauduin MC, Paiardini M, McClure HM, Johnson RP, Silvestri G, Sodora DL Elevated interleukin-7 levels not sufficient to maintain T-cell homeostasis during simian immunodeficiency virus-induced disease progression. Blood, 2003-10-02;103(3):973-9. 2003-10-02 [PMID: 14525780] (Primate - Cebus capucinus (White-headed Capuchin Monkey), Primate - Macaca mulatta (Rhesus Macaque))
• Storek J, Gillespy T, Lu H, Joseph A, Dawson MA, Gough M, Morris J, Hackman RC, Horn PA, Sale GE, Andrews RG, Maloney DG, Kiem HP Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys. Blood, 2003-01-23;101(10):4209-18. 2003-01-23 [PMID: 12543864] (Primate - Papio cynocephalus (Yellow Baboon))

FAQs for IL-7 (HS750). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL7