Human IL-6 Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Conjugate
HRP

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Human IL-6 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-6 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure human IL-6 in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 and antibodies raised against the recombinant protein. Natural human IL-6 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human IL-6.
Specificity
Natural and recombinant human IL-6.
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
D6050B in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-6 Quantikine ELISA Kit

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor.

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

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Publications for IL-6 (D6050B)(790)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Rabbit, Xenograft.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(5)
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Filter By Species
Human
(749)
Mouse
(18)
Rat
(5)
Porcine
(1)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(7)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
Rabbit
(2)
Xenograft
(3)
All Species
Showing Publications 1 - 10 of 790. Show All 790 Publications.
Publications using D6050B Applications Species
Mitsui, E;Kikuchi, S;Okura, T;Tazawa, H;Une, Y;Nishiwaki, N;Kuroda, S;Noma, K;Kagawa, S;Ohara, T;Ohtsuka, J;Ohki, R;Fujiwara, T; Novel treatment strategy targeting interleukin-6 induced by cancer associated fibroblasts for peritoneal metastasis of gastric cancer Scientific reports 2025-01-25 [PMID: 39863722] (Mouse) Mouse
Liu, WC;Wu, MY;Lim, PS; Alterations in Autophagic Function and Endoplasmic Reticulum Stress Markers in the Peripheral Blood Mononuclear Cells of Patients on Hemodialysis International journal of molecular sciences 2025-01-07 [PMID: 39859163] (Human) Human
Son, Y;Kim, M;Lee, D;Kwon, RJ;Kim, K; Roles of 4'-O-Methylalpinum Isoflavone on Activation of Microglia Induced by Oxysterols International journal of molecular sciences 2024-11-27 [PMID: 39684454] (Human) Human
Sarmin, M;Akter, F;Islam, AN;Mahfuz, M;Das, S;Sharifuzzaman, ;Hasan, SMT;Bhuiyan, TR;Rahman, M;Gazi, A;Matin, FB;Tariqujjaman, M;Shahrin, L;Islam, M;Mahmud, AM;Banu, S;Chisti, MJ;Qadri, F;Clemens, JD;Ahmed, T; Cytokine Storm among Bangladeshi adults with COVID-19: A prospective cohort study Heliyon 2024-12-15 [PMID: 39654713] (Human) Human
Kawasaki, R;Sakata, A;Tatsumi, K;Mitani, S;Takeda, M;Kasuda, S;Matsumoto, N;Harada, S;Soeda, T;Nishida, Y;Yoshimura, Y;Shima, M; ?-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats Scientific reports 2024-11-29 [PMID: 39614095] (Human) Human
Matsumoto, H;Annen, S;Mukai, N;Ohshita, M;Ogawa, S;Okita, M;Tanabe, T;Takezawa, M;Nakabayashi, Y;Kikuchi, S;Takeba, J;Sato, N; Association of endotheliopathy with coagulofibrinolytic reactions and disseminated intravascular coagulation after trauma: a retrospective observational study Scientific reports 2024-11-28 [PMID: 39609563] (Human) Human
Abbasifard, M;Kazerooni, K;Taghipour Khaje Sharifi, G;Bagheri-Hosseinabadi, Z;Hajizadeh, MR; Influence of blood trace elements on immune responses and adverse symptoms subsequent to Sinopharm COVID-19 vaccination Scientific reports 2024-11-27 [PMID: 39604541] (Human) Human
Hernández-Lepe, MA;Hernández-Ontiveros, DA;Chávez-Guevara, IA;Ramos-Jiménez, A;Hernández-Torres, RP;López-Fregoso, RJ;Ramos-Lopez, O;Amaro-Gahete, FJ;Muñiz-Salazar, R;Olivas-Aguirre, FJ; Impact of Exercise Training at Maximal Fat Oxidation Intensity on Metabolic and Epigenetic Parameters in Patients with Overweight and Obesity: Study Protocol of a Randomized Controlled Trial Journal of functional morphology and kinesiology 2024-10-31 [PMID: 39584867] (Human) Human
Li, X;Yan, Y;Wang, Z;Hou, J;Meng, Y;Cui, D;Long, Y;Li, M;Yang, R; The link between osteoporosis and frozen shoulder: exploring the therapeutic effect of TAK715 on reversing fibrosis and protecting against osteoporosis via the p38 MAPK signaling pathway BMC musculoskeletal disorders 2024-11-21 [PMID: 39574076] (Human) Human
Devenport, JM;Tran, T;Harris, BR;Fingerman, DF;DeWeerd, RA;Elkhidir, L;LaVigne, D;Fuh, K;Sun, L;Bednarski, JJ;Drapkin, R;Mullen, M;Green, AM; APOBEC3A drives metastasis of high-grade serous ovarian cancer by altering epithelial-to-mesenchymal transition bioRxiv : the preprint server for biology 2024-10-28 [PMID: 39553968] (Human) Human
Show All 790 Publications.

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FAQs for IL-6 (D6050B). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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