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Human IL-10 Quantikine HS ELISA Kit

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human IL-10 Quantikine HS ELISA Kit Summary

Background
The Quantikine HS Human IL-10 Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-10 in serum and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10. Results obtained using natural human IL-10 showed linear curves that are par...allel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.
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Specificity
Natural and recombinant human IL-10
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using HS100C.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-10 Quantikine HS ELISA Kit

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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Publications for IL-10 (HS100C)(56)

We have publications tested in 1 confirmed species: Human.


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Human
(56)
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Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using HS100C Applications Species
Niemelä, O;Bloigu, A;Bloigu, R;Nivukoski, U;Kultti, J;Pohjasniemi, H; Patterns of IgA Autoantibody Generation, Inflammatory Responses and Extracellular Matrix Metabolism in Patients with Alcohol Use Disorder International journal of molecular sciences 2023-08-23 [PMID: 37685930] (Human) Human
Abramiuk, M;Frankowska, K;Ku?ak, K;Tarkowski, R;Mertowska, P;Mertowski, S;Grywalska, E; Possible Correlation between Urocortin 1 (Ucn1) and Immune Parameters in Patients with Endometriosis International journal of molecular sciences 2023-04-24 [PMID: 37175494] (Human) Human
G Elisa, B Tolusso, L Petricca, C Di Mario, MR Gigante, G Ferracciol, S Alivernini Peripheral blood CD4posCD25posFoxP3pos cells and inflammatory cytokines as biomarkers of response in rheumatoid arthritis patients treated with CTLA4-Ig Arthritis Research & Therapy, 2022-06-15;24(1):143. 2022-06-15 [PMID: 35706043] (Human) Human
E Martín-Núñ, A Pérez-Cast, VG Tagua, C Hernández-, C Ferri, N Pérez-Delg, S Rodríguez-, P Cerro-Lópe, Á López-Cast, A Delgado-Mo, VC López-Tarr, MA Arévalo-Gó, A González-L, A Martín-Oli, CC Morales-Es, C Mora-Ferná, J Donate-Cor, JF Navarro-Go Klotho expression in peripheral blood circulating cells is associated with vascular and systemic inflammation in atherosclerotic vascular disease Scientific Reports, 2022-05-19;12(1):8422. 2022-05-19 [PMID: 35590090] (Human) Human
C Griffen, M Duncan, J Hattersley, MO Weickert, A Dallaway, D Renshaw Effects of resistance exercise and whey protein supplementation on skeletal muscle strength, mass, physical function, and hormonal and inflammatory biomarkers in healthy active older men: A randomised, double-blind, placebo-controlled trial Experimental gerontology, 2021-12-09;0(0):111651. 2021-12-09 [PMID: 34896568] (Human) Human
K Kurianowic, M Klatka, A Polak, A Hymos, D B?bnowska, M Podgajna, R Hrynkiewic, O Sierawska, P Nied?wiedz Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression International Journal of Molecular Sciences, 2021-11-09;22(22):. 2021-11-09 [PMID: 34830017] (Human) Human
S Roumelioti, A Veljkovic, PI Georgianos, G Lazarevic, Z Perisic, J Hadzi-Djok, V Liakopoulo, G Kocic Association between Biomarkers of Oxidative Stress and Inflammation with Cardiac Necrosis and Heart Failure in Non-ST Segment Elevation Myocardial Infarction Patients and Various Degrees of Kidney Function Oxidative Medicine and Cellular Longevity, 2021-11-01;2021(0):3090120. 2021-11-01 [PMID: 34760045] (Human) Human
M Bury-Kami?, A Szudy-Szcz, A Nowaczy?sk, O Jankowska-, M Hus, K Kot Chemotherapy-Related Differences in Cognitive Functioning and Their Biological Predictors in Patients with Multiple Myeloma Brain sciences, 2021-09-01;11(9):. 2021-09-01 [PMID: 34573187] (Human) Human
M Wiecek, Z Szygula, J Gradek, J Kusmierczy, J Szymura Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men Biomolecules, 2021-07-16;11(7):. 2021-07-16 [PMID: 34356664] (Human) Human
MM Leite, MT Dutra, MVG da Costa, SS Funghetto, AO Silva, LR de Lima, ICR da Silva, MR Mota, MM Stival Comparative evaluation of inflammatory parameters and substitute insulin resistance indices in elderly women with and without type 2 diabetes mellitus Experimental gerontology, 2021-05-03;150(0):111389. 2021-05-03 [PMID: 33957262] (Human) Human
Show All 56 Publications.

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Bioinformatics

Gene Symbol IL10