Novus Biologicals products are now on

Human IL-10 Quantikine HS ELISA Kit



Product Details

Reactivity HuSpecies Glossary
Applications ELISA

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Human IL-10 Quantikine HS ELISA Kit Summary

The Quantikine HS Human IL-10 Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-10 in serum and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10. Results obtained using natural human IL-10 showed linear curves that are par...allel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.
Show More
Natural and recombinant human IL-10
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using HS100C.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-10 Quantikine HS ELISA Kit

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF


Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA

Publications for IL-10 (HS100C)(56)

We have publications tested in 1 confirmed species: Human.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using HS100C Applications Species
Niemelä, O;Bloigu, A;Bloigu, R;Nivukoski, U;Kultti, J;Pohjasniemi, H; Patterns of IgA Autoantibody Generation, Inflammatory Responses and Extracellular Matrix Metabolism in Patients with Alcohol Use Disorder International journal of molecular sciences 2023-08-23 [PMID: 37685930] (Human) Human
Abramiuk, M;Frankowska, K;Ku?ak, K;Tarkowski, R;Mertowska, P;Mertowski, S;Grywalska, E; Possible Correlation between Urocortin 1 (Ucn1) and Immune Parameters in Patients with Endometriosis International journal of molecular sciences 2023-04-24 [PMID: 37175494] (Human) Human
G Elisa, B Tolusso, L Petricca, C Di Mario, MR Gigante, G Ferracciol, S Alivernini Peripheral blood CD4posCD25posFoxP3pos cells and inflammatory cytokines as biomarkers of response in rheumatoid arthritis patients treated with CTLA4-Ig Arthritis Research & Therapy, 2022-06-15;24(1):143. 2022-06-15 [PMID: 35706043] (Human) Human
E Martín-Núñ, A Pérez-Cast, VG Tagua, C Hernández-, C Ferri, N Pérez-Delg, S Rodríguez-, P Cerro-Lópe, Á López-Cast, A Delgado-Mo, VC López-Tarr, MA Arévalo-Gó, A González-L, A Martín-Oli, CC Morales-Es, C Mora-Ferná, J Donate-Cor, JF Navarro-Go Klotho expression in peripheral blood circulating cells is associated with vascular and systemic inflammation in atherosclerotic vascular disease Scientific Reports, 2022-05-19;12(1):8422. 2022-05-19 [PMID: 35590090] (Human) Human
C Griffen, M Duncan, J Hattersley, MO Weickert, A Dallaway, D Renshaw Effects of resistance exercise and whey protein supplementation on skeletal muscle strength, mass, physical function, and hormonal and inflammatory biomarkers in healthy active older men: A randomised, double-blind, placebo-controlled trial Experimental gerontology, 2021-12-09;0(0):111651. 2021-12-09 [PMID: 34896568] (Human) Human
K Kurianowic, M Klatka, A Polak, A Hymos, D B?bnowska, M Podgajna, R Hrynkiewic, O Sierawska, P Nied?wiedz Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression International Journal of Molecular Sciences, 2021-11-09;22(22):. 2021-11-09 [PMID: 34830017] (Human) Human
S Roumelioti, A Veljkovic, PI Georgianos, G Lazarevic, Z Perisic, J Hadzi-Djok, V Liakopoulo, G Kocic Association between Biomarkers of Oxidative Stress and Inflammation with Cardiac Necrosis and Heart Failure in Non-ST Segment Elevation Myocardial Infarction Patients and Various Degrees of Kidney Function Oxidative Medicine and Cellular Longevity, 2021-11-01;2021(0):3090120. 2021-11-01 [PMID: 34760045] (Human) Human
M Bury-Kami?, A Szudy-Szcz, A Nowaczy?sk, O Jankowska-, M Hus, K Kot Chemotherapy-Related Differences in Cognitive Functioning and Their Biological Predictors in Patients with Multiple Myeloma Brain sciences, 2021-09-01;11(9):. 2021-09-01 [PMID: 34573187] (Human) Human
M Wiecek, Z Szygula, J Gradek, J Kusmierczy, J Szymura Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men Biomolecules, 2021-07-16;11(7):. 2021-07-16 [PMID: 34356664] (Human) Human
MM Leite, MT Dutra, MVG da Costa, SS Funghetto, AO Silva, LR de Lima, ICR da Silva, MR Mota, MM Stival Comparative evaluation of inflammatory parameters and substitute insulin resistance indices in elderly women with and without type 2 diabetes mellitus Experimental gerontology, 2021-05-03;150(0):111389. 2021-05-03 [PMID: 33957262] (Human) Human
Show All 56 Publications.

Reviews for IL-10 (HS100C) (0)

There are no reviews for IL-10 (HS100C). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-10 (HS100C) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-10 Products

Blogs on IL-10.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human IL-10 Quantikine HS ELISA Kit and receive a gift card or discount.


Gene Symbol IL10