Human IL-10 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Human IL-10 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY217B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-10 DuoSet ELISA, 15 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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Publications for IL-10 (DY217B)(225)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse,  Human.


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(215)
Mouse
(3)
Rat
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Showing Publications 1 - 10 of 225. Show All 225 Publications.
Publications using DY217B Applications Species
Westhoff, J;Weber, C;Bachmann, V;Göppert, NE;Peric, M;George, A;Sierka, M;Hoeppener, S;Vilotijevic, I;Schubert, US;Werz, O;Fischer, D; Amino acid-equipped poly(ester amide) nanoparticles for improved encapsulation efficiency of lipophilic anti-inflammatory 6BIGOE based on molecular dynamic simulations International journal of pharmaceutics 2025-09-15 [PMID: 40962080] (Human) Human
Padilha, CS;Olean-Oliveira, T;Figueiredo, C;Dos Santos, VR;Dorneles, GP;Ribeiro, JPJ;Deminice, R;Krüger, K;Rosa-Neto, JC;Lira, FS; CD4+ differentiated T regulatory cells is modified by physical fitness and visceral adipose tissue in young adults-A cross-sectional study Physiological reports 2025-08-01 [PMID: 40853927] (Human) Human
Zhang, W;Zhang, P;Pu, Y;Chen, Z;Su, G;Deng, Y;Zhang, Y;Ji, Y;Huang, Z;Zhou, Q;Luo, X;Lai, Y;Yang, P; DNA 5-Hydroxymethylcytosine Landscape and Transcriptional Profile Highlight the TUBB4B-Mediated Th17/Th1/Treg Imbalance in Behçet's Uveitis Investigative ophthalmology & visual science 2025-08-01 [PMID: 40793858] (Human) Human
Umar, S;Chang, HT;Maienschein-Cline, M;Ravindran, S; Modulation of IRAK4 as a Therapeutic Strategy Against Monosodium Urate- and Xanthine-Induced Inflammation in Macrophages and HepG2 Cells Research square 2025-07-16 [PMID: 40709261] (Human) Human
Khongpraphan, S;Sanongkiet, S;Luangjindarat, C;Thairat, S;Munyoo, B;Chabang, N;Charoensutthivarakul, S;Borwornpinyo, S;Tuchinda, P;Ponpuak, M;Utaisincharoen, P;Pudla, M; ECDD-S16, a synthetic derivative of cleistanthin A, suppresses pyroptosis in Burkholderia pseudomallei-infected U937 macrophages PloS one 2025-07-08 [PMID: 40627773] (Human) Human
Belboul, A;Ashworth, J;Fadel, A;Mcloughlin, J;Mahmoud, A;El Mohtadi, M; Estrogen induces the alternative activation of macrophages through binding to estrogen receptor-alpha Experimental and molecular pathology 2025-05-20 [PMID: 40398084] (Human) Human
Gaggioli, MR;Jones, AG;Panagi, I;Washington, EJ;Loney, RE;Muench, JH;Foster, MW;Brennan, RG;Thurston, TLM;Ko, DC; A single amino acid in the Salmonella effector SarA/SteE triggers supraphysiological activation of STAT3 for anti-inflammatory gene expression Cell reports 2025-04-22 [PMID: 40188438] (Human) Human
Pan, X;Vishnyakova, KS;Chermnykh, ES;Jasko, MV;Zhuravlev, AD;Verkhova, SS;Chegodaev, YS;Popov, MA;Nikiforov, NG;Yegorov, YE; Effect of Free Long-Chain Fatty Acids on Anagen Induction: Metabolic or Inflammatory Aspect? International journal of molecular sciences 2025-03-13 [PMID: 40141208] (Mouse) Mouse
Abdel-Raoof Fouda, M;Abdel-Wahhab, M;Abdelkader, AE;Ibrahim, ME;Elsheikh, TA;Aldeweik, HM;Elfeky, N; Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients BMC infectious diseases 2025-01-30 [PMID: 39885417] (Human) Human
Navas, A;Matzaraki, V;van Eekeren, LE;Blaauw, MJT;Groenendijk, AL;Vos, WAJW;Jacobs-Cleophas, M;Dos Santos, JC;van der Ven, AJAM;Joosten, LAB;Netea, MG; Plasma Proteomic Signature as a Predictor of Age Advancement in People Living With HIV Aging cell 2025-01-15 [PMID: 39812213] (Human) Human
Show All 225 Publications.

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Product General Protocols

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FAQs for IL-10 (DY217B). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol IL10