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Human IL-10 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Human IL-10 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY217B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-10 DuoSet ELISA, 15 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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Publications for IL-10 (DY217B)(198)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse,  Human.


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Human
(189)
Mouse
(2)
Rat
(1)
Transgenic Mouse
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 Human
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Showing Publications 1 - 10 of 198. Show All 198 Publications.
Publications using DY217B Applications Species
Augusto da Costa Teixeira, L;Rocha-Vieira, E;Aparecida Soares, L;Mota de Oliveira, F;Aparecida Oliveira Leopoldino, A;Netto Parentoni, A;Amaral Mendonça, V;Cristina Rodrigues Lacerda, A; The strong inverse association between plasma concentrations of soluble tumor necrosis factor receptors type 1 with adiponectin/leptin ratio in older women Cytokine 2024-01-27 [PMID: 38281360] (Human) Human
Bo?kun, ?;Starosz, A;Kr?towska-Grunwald, A;Wasiluk, T;Walewska, A;Wierzbowska, A;Moniuszko, M;Grubczak, K; Effects of Combinatory In Vitro Treatment with Immune Checkpoint Inhibitors and Cytarabine on the Anti-Cancer Immune Microenvironment in De Novo AML Patients Cancers 2024-01-22 [PMID: 38275902] (Human) Human
Tsai, HF;Tseng, CF;Liang, YL;Wu, PY;Huang, LY;Lin, YH;Lin, LH;Lin, CN;Hsu, KF; Downregulation of Salt-Inducible Kinase 3 Enhances CCL24 Activation in the Placental Environment with Preeclampsia International journal of molecular sciences 2023-12-22 [PMID: 38203391] (Human) Human
Wu, WC;Shiu, C;Tong, TK;Leung, SO;Hui, CW; Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases Journal of immunology research 2023-12-08 [PMID: 38106519] (Human) Human
Jalilian, E;Abolhasani-Zadeh, F;Afgar, A;Samoudi, A;Zeinalynezhad, H;Langroudi, L; Neutralizing tumor-related inflammation and reprogramming of cancer-associated fibroblasts by Curcumin in breast cancer therapy Scientific reports 2023-11-26 [PMID: 38008819] (Human) Human
Nikiforov, NG;Kirichenko, TV;Kubekina, MV;Chegodaev, YS;Zhuravlev, AD;Ilchuk, LA;Nikolaeva, MA;Arefieva, AS;Popov, MA;Verkhova, SS;Bagheri Ekta, M;Orekhov, AN; Macrophages derived from LPS-stimulated monocytes from individuals with subclinical atherosclerosis were characterized by increased pro-inflammatory activity Cytokine 2023-12-01 [PMID: 37918051] (Human) Human
Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D; Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial PloS one 2023-11-02 [PMID: 37917760] (Human) Human
Ren, J;Szombath, G;Vitale-Cross, L;Stroncek, DF;Robey, PG;Hajdara, A;Szalayova, I;Mayer, B;Martin, D;Mezey, E;Nemeth, K; The Potential Use of THP-1, a Monocytic Leukemia Cell Line, to Predict Immune-Suppressive Potency of Human Bone-Marrow Stromal Cells (BMSCs) In Vitro: A Pilot Study International journal of molecular sciences 2023-08-26 [PMID: 37686058] (Human) Human
Meunier, M;Spillmann, A;Rousseaux, C;Schwamborn, K;Hanson, M; An oral cholera vaccine in the prevention and/or treatment of inflammatory bowel disease PloS one 2023-08-28 [PMID: 37639428] (Human) Human
Kr?towska-Grunwald, A;Sawicka-?ukowska, M;Kowalska, M;Basaj, A;Krawczuk-Rybak, M;Moniuszko, M;Grubczak, K; Significance of Th17 and Treg in Treatment Efficacy and Outcome in Pediatric Acute Lymphoblastic Leukemia International journal of molecular sciences 2023-08-01 [PMID: 37569699] (Human) Human
Show All 198 Publications.

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How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol IL10