Human FGF basic/FGF2/bFGF DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human FGF basic/FGF2/bFGF DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FGF2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human FGF basic/FGF2/bFGF DuoSet ELISA, 15 Plate

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Publications for FGF basic/FGF2/bFGF (DY233)(29)

Publications using DY233

• Jiang, J;Röper, L;Fuchs, F;Hanschen, M;Failer, S;Alageel, S;Cong, X;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P; Bone Regenerative Effect of Injectable Hypoxia Preconditioned Serum-Fibrin (HPS-F) in an Ex Vivo Bone Defect Model International journal of molecular sciences 2024-05-13 [PMID: 38791352] (Human)
• Jiang, J;Altammar, J;Cong, X;Ramsauer, L;Steinbacher, V;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P; Hypoxia Preconditioned Serum (HPS) Promotes Proliferation and Chondrogenic Phenotype of Chondrocytes In Vitro International journal of molecular sciences 2023-06-21 [PMID: 37445617] (Human)
• Berlin, F;Mogren, S;Ly, C;Ramu, S;Hvidtfeldt, M;Uller, L;Porsbjerg, C;Andersson, CK; Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells Cells 2023-05-22 [PMID: 37408273] (Human)
• J Jiang, X Cong, S Alageel, U Dornseifer, AF Schilling, E Hadjipanay, HG Machens, P Moog In Vitro Comparison of Lymphangiogenic Potential of Hypoxia Preconditioned Serum (HPS) and Platelet-Rich Plasma (PRP) International Journal of Molecular Sciences, 2023-01-19;24(3):. 2023-01-19 [PMID: 36768283] (Human)
• I Petit, A Levy, S Estrach, CC Féral, AG Trentin, F Dingli, D Loew, J Qu, H Zhou, C Théry, C Prunier, D Aberdam, O Ferrigno Fibroblast growth factor-2 bound to specific dermal fibroblast-derived extracellular vesicles is protected from degradation Scientific Reports, 2022-12-22;12(1):22131. 2022-12-22 [PMID: 36550142] (Human)
• S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis Journal of ovarian research, 2022-07-26;15(1):87. 2022-07-26 [PMID: 35883110] (Human)
• AL Graça, RMA Domingues, I Calejo, M Gómez-Flor, ME Gomes Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model International Journal of Molecular Sciences, 2022-03-09;23(6):. 2022-03-09 [PMID: 35328370] (Human)
• J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy Nature Communications, 2021-02-17;12(1):1087. 2021-02-17 [PMID: 33597530] (Human)
• DL Vitale, I Caon, A Parnigoni, I Sevic, FM Spinelli, A Icardi, A Passi, D Vigetti, L Alaniz Initial Identification of UDP-Glucose Dehydrogenase as a Prognostic Marker in Breast Cancer Patients, Which Facilitates Epirubicin Resistance and Regulates Hyaluronan Synthesis in MDA-MB-231 Cells Biomolecules, 2021-02-09;11(2):. 2021-02-09 [PMID: 33572239] (Human)
• Y Ikegami, H Mizumachi, K Yoshida, H Ijima Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine Regenerative Therapy, 2020-11-01;15(0):236-242. 2020-11-01 [PMID: 33426224] (Human)
• M Tutter, C Schug, KA Schmohl, S Urnauer, C Kitzberger, N Schwenk, M Petrini, C Zach, S Ziegler, P Bartenstei, WA Weber, G Multhoff, E Wagner, LH Lindner, PJ Nelson, C Spitzweg Regional hyperthermia enhances mesenchymal stem cell recruitment to tumor stroma: Implications for mesenchymal stem cell-based tumor therapy Mol Ther, 2020-10-15;0(0):. 2020-10-15 [PMID: 33068779] (Human)
• M Aliabouzar, A Jivani, X Lu, OD Kripfgans, JB Fowlkes, ML Fabiilli Standing wave-assisted acoustic droplet vaporization for single and dual payload release in acoustically-responsive scaffolds Ultrason Sonochem, 2020-03-26;66(0):105109. 2020-03-26 [PMID: 32248042] (N/A)
• A Islam, I Urbarova, JA Bruun, I Martinez-Z Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells Eur Cell Mater, 2019-02-20;37(0):153-174. 2019-02-20 [PMID: 30785213] (Human)
• U Hopfner, MM Aitzetmuel, P Ne beta bach, MS Hu, HG Machens, ZN Maan, D Duscher Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing Stem Cells Int, 2018-12-19;2018(0):1353085. 2018-12-19 [PMID: 30662467] (Human)
• MS Chen, TJ Wang, HC Lin, B Thierry Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells New biotechnology, 2018-11-20;0(0):. 2018-11-20 [PMID: 30465908] (Human)
• AC Finato, TF Fraga-Silv, AUC Prati, AA de Souza J, BF Mazzeu, LG Felippe, RA Pinto, MA Golim, MSP Arruda, M Furlan, J Venturini Crude leaf extracts of Piperaceae species downmodulate inflammatory responses by human monocytes PLoS ONE, 2018-06-20;13(6):e0198682. 2018-06-20 [PMID: 29924840] (Human)
• F Klatte-Sch, T Schmidt, M Uckert, S Scheffler, U Kalus, M Rojewski, H Schrezenme, A Pruss, B Wildemann Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study Int J Mol Sci, 2018-01-10;19(1):. 2018-01-10 [PMID: 29320421] (Human)
• J Miku?a-Pie, P Uruski, S Szubert, K Maksin, R Moszy?ski, D Szpurek, A Wo?niak, S Sajdak, A Tykarski, K Ksi??ek The Proangiogenic Capabilities of Malignant Ascites Generated by Aggressive Ovarian Tumors Biomed Res Int, 2017-09-20;2017(0):2592496. 2017-09-20 [PMID: 29085834] (Human)
• J Venturini, RS Cavalcante, DV Moris, M de Assis G, AD Levorato, KH Dos Reis, MSP de Arruda, RP Mendes Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis Acta Trop., 2017-06-09;0(0):. 2017-06-09 [PMID: 28606816] (Human)
• LA Ahrens, D Vonwil, J Christense, VP Shastri Gelatin device for the delivery of growth factors involved in endochondral ossification PLoS ONE, 2017-04-05;12(4):e0175095. 2017-04-05 [PMID: 28380024] (Human)
• David M, Kelly E, Zoellner H Opposite cytokine synthesis by fibroblasts in contact co-culture with osteosarcoma cells compared with transwell co-cultures. Cytokine, 2013-03-19;62(1):48-51. 2013-03-19 [PMID: 23523091] (Human)
• Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011-07-13;475(7355):226-30. 2011-07-13 [PMID: 21753853] (Mouse)
• Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity. Brain, 2010-07-21;133(0):2232-47. 2010-07-21 [PMID: 20659956] (Human)
• Peterson JE, Zurakowski D, Italiano JE Normal ranges of angiogenesis regulatory proteins in human platelets. Am. J. Hematol., 2010-07-01;85(7):487-93. 2010-07-01 [PMID: 20575035] (Human)
• Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment. FASEB J., 2010-05-19;24(10):3782-95. 2010-05-19 [PMID: 20484670] (Human)
• Parsi MK, Adams JR, Whitelock J LTBP-2 has multiple heparin/heparan sulfate binding sites. Matrix Biol., 2010-04-09;29(5):393-401. 2010-04-09 [PMID: 20382221] (Human)
• Riekstina U, Muceniece R, Cakstina I, Muiznieks I, Ancans J Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions. Cytotechnology, 2009-02-14;58(3):153-62. 2009-02-14 [PMID: 19219561] (Human)
• Shi S, Mercer S, Eckert GJ, Trippel SB Growth factor regulation of growth factors in articular chondrocytes. J. Biol. Chem., 2009-01-09;284(11):6697-704. 2009-01-09 [PMID: 19136669] (Human)
• Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer. Br. J. Cancer, 2006-02-27;94(4):524-31. 2006-02-27 [PMID: 16450002] (Human)

FAQs for FGF basic/FGF2/bFGF (DY233). (Showing 1 - 2 of 2 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FGF2