MATN3 Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MATN3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Matrilin-3
  Ala35-Arg481
  Accession # AAH71224
• Specificity: Detects mouse Matrilin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human Matrilin-3 is observed and 5% cross-reactivity with recombinant mouse (rm) Matrilin-2 and rmMatrilin-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MATN3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MATN3 Antibody

• DIPOA
• EDM5
• HOA
• matrilin 3
• Matrilin3
• Matrilin-3
• NDUFC2
• OADIP
• OS2

Background

Matrilin-3 is a 50-60 kDa extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is primarily expressed in cartilage and functions as a bridging component between proteins of the collagenous matrix (1-3). The mouse Matrilin-3 cDNA encodes a 481 amino acid (aa) precursor with a 27 aa signal sequence, an N-terminal VWA domain, four tandem EGF-like repeats, and a C-terminal coiled-coil domain (4). The Matrilins differ in the number of VWA domains (one or two) and EGF-like repeats (one, three, four, or ten) they contain. Mouse Matrilin-3 shares 82% aa sequence identity with human Matrilin-3. Within the first VWA domain, mouse Matrilin-3 shares approximately 51% aa sequence identity with mouse Matrilin-1, -2, and -4. The coiled-coil domain of Matrilin-3 mediates disulfide-linked homo-oligomerization, with tetramer formation being the most dominant (5-7). It can also assemble into hetero-oligomers with Matrilin-1 (5-7). Matrilin-3 is more plentiful than Matrilin-1 in the proliferative zone of the growth plate, whereas the reverse is true in the maturation zone (5). Matrilin-3 interacts directly with Collagen IX and COMP (8, 9). In the absence of Collagen IX, the expression of Matrilin-3 is unchanged, although it is retained inside chondrocytes and is not incorporated into the matrix (9). Intracellular retention of Matrilin-3 also occurs with particular point mutations in the VWA domain that results in multiple epiphyseal dysplasia (11-13). In contrast, a point mutation in the first EGF-like repeat which has been linked to hand osteoarthritis does not prevent Matrilin-3 secretion (13). Matrilin-3 knockout mice do not display any obvious abnormalities, suggesting that other molecules may compensate for the lack of Matrilin-3 (10).

1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.
2. Deak, F. et al. (1999) Matrix Biol. 18:55.
3. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.
4. Wagener, R. et al. (1997) FEBS Lett. 413:129.
5. Zhang, Y. and Q. Chen (2000) J. Biol. Chem. 275:32628.
6. Klatt, A.R. et al. (2000) J. Biol. Chem. 275:3999.
7. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
8. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
9. Budde, B. et al. (2005) Mol. Cell. Biol. 25:10465.
10. Ko, Y. et al. (2004) Mol. Cell. Biol. 24:1691.
11. Jackson, G.C. et al. (2004) J. Med. Genet. 41:52.
12. Cotterill, S.L. et al. (2005) Hum. Mutat. 26:557.
13. Otten, C. et al. (2005) J. Med. Genet. 42:774.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MATN3 Antibody (AF3357)(4)

Publications using AF3357

• KA Piróg, EP Dennis, CL Hartley, RM Jackson, J Soul, JM Schwartz, JF Bateman, RP Boot-Handf, MD Briggs XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease PLoS Genet., 2019-07-01;15(7):e1008215. 2019-07-01 [PMID: 31260448] (IHC, Mouse)
• P. A. Bell, E. P. Dennis, C. L. Hartley, R. M. Jackson, A. Porter, R. P. Boot-Handford et al. Mesencephalic astrocyte-derived neurotropic factor is an important factor in chondrocyte ER homeostasis Cell Stress and Chaperones 1/1/2019 [PMID: 30543055]
• Posey KL, Veerisetty AC, Liu P, Wang HR, Poindexter BJ, Bick R, Alcorn JL, Hecht JT An inducible cartilage oligomeric matrix protein mouse model recapitulates human pseudoachondroplasia phenotype. Am. J. Pathol., 2009-09-17;175(4):1555-63. 2009-09-17 [PMID: 19762713] (IHC, Mouse)
• Posey KL, Hankenson K, Veerisetty AC, Bornstein P, Lawler J, Hecht JT Skeletal abnormalities in mice lacking extracellular matrix proteins, thrombospondin-1, thrombospondin-3, thrombospondin-5, and type IX collagen. Am. J. Pathol., 2008-05-08;172(6):1664-74. 2008-05-08 [PMID: 18467703] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: MATN3
• Uniprot:
  • AAH71224()