Human COMP DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human COMP DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: COMP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human COMP DuoSet ELISA, 15 Plate

• cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• cartilage oligomeric matrix protein
• cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• COMP
• EDM1
• EPD1
• MED
• MEDMGC131819
• MGC149768
• PSACH
• pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
• THBS5
• Thrombospondin5
• Thrombospondin-5
• TSP5

Background

COMP (Cartilage Oligomeric Matrix Protein), also known as Thrombospondin-5, is a multidomain calcium binding protein that associates with other extracellular matrix molecules. COMP associates into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands. An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrins a5b1 or aVb3. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Several mutations in COMP are associated with skeletal dysplasias.

Publications for COMP/Thrombospondin-5 (DY3134)(2)

Publications using DY3134

• Hanitrarimalala, V;Bednarska, I;Murakami, T;Papadakos, KS;Blom, AM; Intracellular cartilage oligomeric matrix protein augments breast cancer resistance to chemotherapy Cell death & disease 2024-07-04 [PMID: 38965233] (Human)
• K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration PLoS ONE, 2020-08-07;15(8):e0236283. 2020-08-07 [PMID: 32764794] (Human)

FAQs for COMP/Thrombospondin-5 (DY3134). (Showing 1 - 2 of 2 FAQs).

1. I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
   • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: COMP