Human C-Peptide Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human C-Peptide Quantikine ELISA Kit Summary

• Background: The Quantikine Human Insulin C-Peptide Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human Insulin C-Peptide in cell culture supernates, serum, plasma, and urine. It contains synthetic human Insulin C-Peptide and has been shown to accurately quantitate the peptide. Results obtained using natural human Insulin C-Peptide showed linear curves that were parallel to the standard... curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Insulin C-Peptide.
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• Specificity: Natural and synthetic human Insulin C-Peptide.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: INS

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human C-Peptide Quantikine ELISA Kit

• C-P
• C-Peptide
• IDDM2
• ILPR
• Insulin C-Peptide
• insulin
• IRDN
• MODY10
• Proinsulin C-Peptide
• proinsulin

Background

Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for C-Peptide (DICP00)(5)

Publications using DICP00

• Ghade, NS;Thappa, DK;Lona, J;Krishnan, AR;Sonar, SM; Comparative physicochemical and structural characterisation studies establish high biosimilarity between BGL-ASP and reference insulin aspart Scientific reports 2024-02-20 [PMID: 38378730] (Human)
• Hernandez, JD;Li, T;Ghannam, H;Rau, CM;Masuda, MY;Madura, JA;Jacobsen, EA;Filippis, E; Linking adipose tissue eosinophils, interleukin-4, and leptin in human obesity and insulin resistance JCI insight 2024-01-11 [PMID: 38206766] (Bioassay, Human)
• F Hoel, A Hoel, IK Pettersen, IG Rekeland, K Risa, K Alme, K Sørland, A Fosså, K Lien, I Herder, HL Thürmer, ME Gotaas, C Schäfer, RK Berge, K Sommerfelt, HP Marti, O Dahl, O Mella, Ø Fluge, KJ Tronstad A map of metabolic phenotypes in patients with myalgic encephalomyelitis/chronic fatigue syndrome JCI Insight, 2021-08-23;6(16):. 2021-08-23 [PMID: 34423789] (Human)
• KM Mohany, O Al Rugaie, O Al-Wutayd, A Al-Nafeesa Investigation of the levels of circulating miR-29a, miR-122, sestrin 2 and inflammatory markers in obese children with/without type 2 diabetes: a case control study BMC endocrine disorders, 2021-08-03;21(1):152. 2021-08-03 [PMID: 34344352] (Human)
• Z Ren, Z Yang, Y Lu, R Zhang, H Yang Anti?glycolipid disorder effect of epigallocatechin?3?gallate on high?fat diet and STZ?induced T2DM in mice Mol Med Rep, 2020-03-26;21(6):2475-2483. 2020-03-26 [PMID: 32236613] (Mouse)

FAQs for C-Peptide (DICP00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: INS