Human Angiopoietin-1 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Angiopoietin-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ANGPT1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Angiopoietin-1 DuoSet ELISA, 15 Plate

• AGP1
• AGPT
• Ang1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Publications for Angiopoietin-1 (DY923)(41)

Publications using DY923

• Mayhew, JA;Witten, AJ;Bond, CA;Opoka, RO;Bangirana, P;Conroy, AL;Hernandez-Alvarado, N;Schleiss, MR;John, CC; Cytomegalovirus reactivation and acute and chronic complications in children with cerebral malaria: a prospective cohort study Malaria journal 2025-02-17 [PMID: 39962580] (Human)
• López-Guzmán, C;García, AM;Marín, P;Vásquez, AM; Assessment of the Integrity and Function of Human Term Placental Explants in Short-Term Culture Methods and protocols 2024-02-15 [PMID: 38392690] (Human)
• Agyekum, JA;Yeboah, K; Peripheral sensory neuropathy is associated with circulating angiopoietins in type 2 diabetes patients in Ghana Journal of clinical & translational endocrinology 2023-12-01 [PMID: 37822668] (Human)
• S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis Journal of ovarian research, 2022-07-26;15(1):87. 2022-07-26 [PMID: 35883110] (Human)
• CE Lekpor, F Botchway, KA Kusi, AA Adjei, MD Wilson, JK Stiles, NO Wilson Angiogenic and angiostatic factors present in the saliva of malaria patients Malaria Journal, 2022-07-14;21(1):220. 2022-07-14 [PMID: 35836234] (Human)
• J Kaur, K Kamboj, AK Yadav, P Kaur, V Kumar, V Jha Cholecalciferol supplementation and angiogenic markers in chronic kidney disease PLoS ONE, 2022-06-03;17(6):e0268946. 2022-06-03 [PMID: 35657784] (Human)
• M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus Cardiovascular Diabetology, 2022-05-12;21(1):72. 2022-05-12 [PMID: 35549955] (Human)
• J Ohmes, MD Mikkelsen, TT Nguyen, VHN Tran, S Meier, MS Nielsen, M Ding, A Seekamp, AS Meyer, S Fuchs Depolymerization of fucoidan with endo-fucoidanase changes bioactivity in processes relevant for bone regeneration Carbohydrate polymers, 2022-02-24;286(0):119286. 2022-02-24 [PMID: 35337530] (Human)
• V Tran, AM Weckman, VM Crowley, LS Cahill, K Zhong, A Cabrera, RE Elphinston, V Pearce, M Madanitsa, L Kalilani-P, V Mwapasa, C Khairallah, AL Conroy, FO Ter Kuile, JG Sled, KC Kain The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy EBioMedicine, 2021-11-07;73(0):103683. 2021-11-07 [PMID: 34758414] (Mouse)
• Qimei Gong, Junyu Zeng, Xufang Zhang, Yihua Huang, Chanchan Chen, Jingjing Quan et al. Effect of erythropoietin on angiogenic potential of dental pulp cells Experimental and Therapeutic Medicine 2021-07-28 [PMID: 34447472] (Human)
• E Charles, BL Dumont, S Bonneau, PE Neagoe, L Villeneuve, A Räkel, M White, MG Sirois Angiopoietin 1 release from human neutrophils is independent from neutrophil extracellular traps (NETs) BMC immunology, 2021-08-03;22(1):51. 2021-08-03 [PMID: 34344299] (Human)
• R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut? Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma Scientific Reports, 2021-06-23;11(1):13100. 2021-06-23 [PMID: 34162919] (Human)
• T Eichhorn, I Linsberger, L Lauková, C Tripiscian, B Fendl, R Weiss, F König, G Valicek, G Miestinger, C Hörmann, V Weber Analysis of Inflammatory Mediator Profiles in Sepsis Patients Reveals That Extracellular Histones Are Strongly Elevated in Nonsurvivors Mediators of Inflammation, 2021-03-17;2021(0):8395048. 2021-03-17 [PMID: 33790693] (Human)
• AG Vassiliou, C Keskinidou, E Jahaj, P Gallos, I Dimopoulou, A Kotanidou, SE Orfanos ICU Admission Levels of Endothelial Biomarkers as Predictors of Mortality in Critically Ill COVID-19 Patients Cells, 2021-01-19;10(1):. 2021-01-19 [PMID: 33477776] (Human)
• J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment Mar Drugs, 2020-09-21;18(9):. 2020-09-21 [PMID: 32967359] (Human)
• J Yu, K Huang, J Pan, J Shen, R Zhan Significance of Serum Angiopoietin-2 in Patients with Hemorrhage in Adult-Onset Moyamoya Disease Biomed Res Int, 2020-08-04;2020(0):8209313. 2020-08-04 [PMID: 32802878] (Human)
• R Zhang, U Rai, NBM Ibrahim, Y Amazouz, J Soo, AL Conroy, S Namasopo, RO Opoka, R Bhargava, MT Hawkes Dysregulation of angiopoietin-Tie-2 axis in ugandan children hospitalized with pneumonia Cytokine, 2020-06-26;133(0):155175. 2020-06-26 [PMID: 32599540] (Human)
• G Varricchi, S Loffredo, L Bencivenga, AL Ferrara, G Gambino, N Ferrara, A de Paulis, G Marone, G Rengo Angiopoietins, Vascular Endothelial Growth Factors and Secretory Phospholipase A2 in Ischemic and Non-Ischemic Heart Failure J Clin Med, 2020-06-19;9(6):. 2020-06-19 [PMID: 32575548] (Human)
• PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes Dis. Markers, 2020-01-13;2020(0):6163487. 2020-01-13 [PMID: 32399088] (Human)
• K Shuto, M Mori, C Kosugi, K Narushima, S Nakabayash, T Fujisiro, A Sato, K Hayano, H Shimizu, K Koda Hepatic blood flow by perfusion computed tomography as an imaging biomarker for patients with gastric cancer Oncol Lett, 2019-01-25;17(3):3267-3276. 2019-01-25 [PMID: 30867759] (Human)
• KD Tang, J Liu, PJ Russell, JA Clements, MT Ling Gamma-Tocotrienol Induces Apoptosis in Prostate Cancer Cells by Targeting the Ang-1/Tie-2 Signalling Pathway Int J Mol Sci, 2019-03-07;20(5):. 2019-03-07 [PMID: 30866453] (Human)
• H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells Reprod Biol, 2019-03-06;0(0):. 2019-03-06 [PMID: 30852242] (Human)
• JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study BMC Pediatr, 2018-03-23;18(1):118. 2018-03-23 [PMID: 29571293] (Human)
• SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh Tie2 protects the vasculature against thrombus formation in systemic inflammation J. Clin. Invest., 2018-03-05;0(0):. 2018-03-05 [PMID: 29360642] (Mouse)
• Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha. PLoS ONE, 2015-09-14;10(9):e0137612. 2015-09-14 [PMID: 26367137] (Human)
• McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women. PLoS ONE, 2015-08-06;10(8):e0134619. 2015-08-06 [PMID: 26247200] (Human)
• Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta. PLoS Negl Trop Dis, 2015-06-19;9(6):e0003824. 2015-06-19 [PMID: 26090803] (Human)
• Ishtiaq S, Edwards S, Sankaralingam A, Evans B, Elford C, Frost M, Fogelman I, Hampson G The effect of nitrogen containing bisphosphonates, zoledronate and alendronate, on the production of pro-angiogenic factors by osteoblastic cells. Cytokine, 2014-11-17;71(2):154-60. 2014-11-17 [PMID: 25461393] (Human)
• Kim Aubin, Caroline Vincent, Maryse Proulx, Dominique Mayrand, Julie Fradette Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products Acta Biomaterialia 2015-01-01 [PMID: 25278444] (Human)
• Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-01-20;14(0):35. 2014-01-20 [PMID: 24444080] (Human)
• Giri H, Chandel S, Dwarakanath L, Sreekumar S, Dixit M Increased endothelial inflammation, sTie-2 and arginase activity in umbilical cords obtained from gestational diabetic mothers. PLoS ONE, 2013-12-20;8(12):e84546. 2013-12-20 [PMID: 24376824] (Human)
• Conroy AL, Liles WC, Molyneux ME Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. PLoS ONE, 2011-12-12;6(12):e28540. 2011-12-12 [PMID: 22174834] (Human)
• Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study. PLoS ONE, 2010-12-29;5(12):e15291. 2010-12-29 [PMID: 21209923] (Human)
• Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment. FASEB J., 2010-05-19;24(10):3782-95. 2010-05-19 [PMID: 20484670] (Human)
• Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy. Placenta, 2010-03-24;31(6):545-8. 2010-03-24 [PMID: 20338637] (Human)
• Boneberg EM, Legler DF, Hoefer MM, Ohlschlegel C, Steininger H, Fuzesi L, Beer GM, Dupont-Lampert V, Otto F, Senn HJ, Furstenberger G Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer. Br. J. Cancer, 2009-08-18;101(4):605-14. 2009-08-18 [PMID: 19672262] (Human)
• Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia. Am. J. Hypertens., 2009-05-28;22(8):891-5. 2009-05-28 [PMID: 19478794] (Human)
• Ferraro B, Cruz YL, Coppola D, Heller R Intradermal delivery of plasmid VEGF(165) by electroporation promotes wound healing. Mol. Ther., 2009-02-24;17(4):651-7. 2009-02-24 [PMID: 19240696] (Rat)
• Chung I, Han G, Seshadri M, Gillard BM, Yu WD, Foster BA, Trump DL, Johnson CS Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo. Cancer Res., 2009-01-13;69(3):967-75. 2009-01-13 [PMID: 19141646] (Mouse)
• Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H Serum levels of angiogenic factors and their prognostic relevance in bladder cancer. Pathol. Oncol. Res., 2008-09-20;15(2):193-201. 2008-09-20 [PMID: 18807212] (Human)
• van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients. Thorax, 2008-06-17;63(10):903-9. 2008-06-17 [PMID: 18559364] (Human)

FAQs for Angiopoietin-1 (DY923). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANGPT1