Human ACE Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human ACE Quantikine ELISA Kit Summary

• Background: The Quantikine Human ACE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACE in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed ectodomain of recombinant human sACE, and antibodies raised against the recombinant factor. Natural human ACE showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikin...e kit standards, indicating that this kit can be used to determine relative levels of natural human ACE.
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• Specificity: Natural and recombinant human ACE. In addition to soluble ACE, this kit recognizes membrane-bound forms from lysates of cultured cells and supernates.
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ACE

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human ACE Quantikine ELISA Kit

• ACE
• ACE1angiotensin converting enzyme, somatic isoform
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• carboxycathepsin
• CD143 antigen
• CD143
• DCP
• DCP1
• DCP1angiotensin-converting enzyme
• dipeptidyl carboxypeptidase 1
• Dipeptidyl carboxypeptidase I
• EC 3.2.1.-
• EC 3.4.15.1
• Kininase II
• MGC26566
• MVCD3
• peptidase P
• testicular ECA

Background

ACE and ACE-2, two cell surface proteases, are important regulators of the renin-angiotensin system (RAS), which plays a key role in maintaining blood pressure homeostasis and fluid salt balance in mammals. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. ACE exists in two isoforms. Somatic ACE comprises two highly similar protease domains whereas germinal ACE comprises a single protease domain. ACE-2 consists of a single protease domain and is an essential regulator of heart and lung function. It is also a cellular receptor for the Spike protein of SARS cornonavirus that causes severe acute respiratory syndrome.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for ACE/CD143 (DACE00)(5)

Publications using DACE00

• PL Brito, AF Dos Santos, H Chweih, ME Favero, EMF Gotardo, JAF Silva, FC Leonardo, CF Franco-Pen, MG de Oliveir, WA Ferreira, BC Zaidan, A Billis, G Baldanzi, DA Mashima, E Antunes, STO Saad, FF Costa, N Conran Reduced blood pressure in sickle cell disease is associated with decreased angiotensin converting enzyme (ACE) activity and is not modulated by ACE inhibition PLoS ONE, 2022;17(2):e0263424. 2022-01-01 [PMID: 35113975] (Human)
• X Xu, L Shi, X Ma, H Su, G Ma, X Wu, K Ying, R Zhang RhoA-Rho associated kinase signaling leads to renin-angiotensin system imbalance and angiotensin converting enzyme 2 has a protective role in acute pulmonary embolism Thromb. Res., 2019;176(0):85-94. 2019-01-01 [PMID: 30784777] (Human)
• S Yasar, VR Varma, GC Harris, MC Carlson Associations of Angiotensin Converting Enzyme-1 and Angiotensin II Blood Levels and Cognitive Function J. Alzheimers Dis., 2018;0(0):. 2018-01-01 [PMID: 29660936] (Human)
• Wang P, Holst C, Wodzig W, Andersen M, Astrup A, van Baak M, Larsen T, Jebb S, Kafatos A, Pfeiffer A, Martinez J, Handjieva-Darlenska T, Kunesova M, Viguerie N, Langin D, Saris W, Mariman E Circulating ACE is a predictor of weight loss maintenance not only in overweight and obese women, but also in men. Int J Obes (Lond), 2012;36(12):1545-51. 2012-01-01 [PMID: 22270380] (Human)
• Nielsen HM, Londos E, Minthon L, Janciauskiene SM Soluble adhesion molecules and angiotensin-converting enzyme in dementia. Neurobiol. Dis., 2007;26(1):27-35. 2007-01-01 [PMID: 17270454] (Human)

Bioinformatics

• Gene Symbol: ACE