Home » HtrA3 » HtrA3 Antibodies » HtrA3 Antibody

HtrA3 Antibody

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: HtrA3 Antibody [NB600-1151] - Western bot analysis of HtrA3 in rat heart lysate using NB600-1151 at 2 ug/ml.
Western Blot: HtrA3 Antibody [NB600-1151] - Western bot analysis of HtrA3 in rat heart lysate using NB600-1151 at 2 ug/ml.
Western Blot: HtrA3 Antibody [NB600-1151] - analysis of HtrA1/2/3/4 in heart lysate from A) human, B) mouse and C) rat using this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, FeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

100% Guarantee on every product

HtrA3 Antibody Summary

Immunogen
Amino acids 273-287 (IVSTAQREGRELGLR) of human HtrA3 were used as immunogen.
Localization
Secreted
Specificity
The amino acid sequence is 100% homologous in HtrA3, 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4.
Predicted Species
Feline (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTRA3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Western Blot: Detects a band of approximately 43 kDa (predicted molecular weight: 49 kDa). Immunocytochemistry/Immunofluorescence: See Beleford et al for more details.
Publications
Read Publication using NB600-1151.

Reactivity Notes

Predicted to react with Chicken and Cat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.2% Gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for HtrA3 Antibody

  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.108
  • High-temperature requirement factor A3
  • HtrA serine peptidase 3
  • Pregnancy-related serine protease
  • probable serine protease HTRA3
  • Prsp
  • serine protease HTRA3
  • Tasp

Background

HtrA3 belongs to the human high-temperature requirement factor A (HtrA) family. HtrA3 has two isoforms (long and short), which are produced by alternative splicing. Similar to HtrA1, HtrA3 binds to various TGF-beta proteins and inhibits the signaling of BMP-4, -2 and TGF-beta 1. HtrA3 does not inhibit signaling originated from a constitutively active BMP receptor, indicating that the inhibition occurs upstream of the cell surface receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

PDZD11 Antibody
AF5977
Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PDZD11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5977) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDZD11 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
NHE6/SLC9A6
Antibody
NBP2-32463
Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-32463] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-32463] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-32463] - Staining in human cerebral cortex and liver tissues using anti-SLC9A6 antibody. Corresponding SLC9A6 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
HTRA1/PRSS11
Antibody
NBP2-23869
Western Blot: HTRA1/PRSS11 Antibody [NBP2-23869] - Analysis of HtrA1 in human A) HeLa cell lysate (1 minute exposure) and B) human 293 cell lysate (15 minute exposure) using NBP2-23869 at 1:500.
Simple Western: HTRA1/PRSS11 Antibody [NBP2-23869] - Simple Western lane view shows a specific band for htrA1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(2 Publications)
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, Simple Western, ICC/IF
CXCL7/NAP-2
Antibody (59418) [Unc ...
MAB393
Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Recombinant Human CXCL7/NAP‑2 (Catalog #393‑NP) induces myeloperox­idase release in the cytochalasin‑B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). The ND50 is typically 15‑20 µg/mL in the presence of cytochalasin‑B.
(1 Review)
(5 Publications)
Species: Hu
Applications: WB, Simple Western, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
TRAP220/MED1
Antibody
AF5520
Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TRAP220 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5520) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRAP220 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
(1 Review)
Species: Hu, Mu
Applications: WB
RKIP/PBP Antibody
(SC58-09)
NBP2-67560
Western Blot: RKIP/PBP Antibody (SC58-09) [NBP2-67560] - Analysis of PBP on mouse brain lysates using anti-PBP antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: RKIP/PBP Antibody (SC58-09) [NBP2-67560] - Staining PBP in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RKIP/PBP Antibody (SC58-09) [NBP2-67560] - Analysis of paraffin-embedded mouse kidney tissue using anti-PBP antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P, IP
HTRA2/Omi Antibody
[Unconjugated]
AF1458
Western blot shows lysates of PC‑12 rat adrenal pheochromocytoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for HTRA2/Omi at approximately 36 and 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
HTRA2/Omi was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for HTRA2/Omi at approximately 51 kDa (precursor) and 41 kDa (processed) (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Review)
(21 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
Polycystin-1/PKD1
Antibody (PKS161f/B7)
NBP2-50247
Polycystin-1/PKD1 Antibody (PKS161f/B7)
Species: Hu
Applications: WB, IHC
TCIRG1 Antibody
NBP1-89333
Immunocytochemistry/Immunofluorescence: TCIRG1 Antibody [NBP1-89333] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TCIRG1 Antibody [NBP1-89333] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
CYP27A1 Antibody
NBP2-32433
Western Blot: CYP27A1 Antibody [NBP2-32433] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody [NBP2-32433] - Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.
Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-32433] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
transgelin 2
Antibody
NBP1-89722
Western Blot: transgelin 2 Antibody [NBP1-89722] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: transgelin 2 Antibody [NBP1-89722] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: transgelin 2 Antibody [NBP1-89722] - Staining of human tonsil shows moderate to strong positivity in germinal center cells.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Aquaporin-8
Antibody (1A1)
H00000343-M01
Western Blot: Aquaporin-8 Antibody (1A1) [H00000343-M01] - AQP8 monoclonal antibody (M01), clone 1A1. Analysis of AQP8 expression in PC-12.
Western Blot: Aquaporin-8 Antibody (1A1) [H00000343-M01] - AQP8 monoclonal antibody (M01), clone 1A1. Analysis of AQP8 expression in HeLa.
Western Blot: Aquaporin-8 Antibody (1A1) [H00000343-M01] - AQP8 monoclonal antibody (M01), clone 1A1 Analysis of AQP8 expression in Hela S3 NE.
(2 Publications)
Species: Hu, Rt
Applications: WB, ELISA
NDFIP2 Antibody
NBP1-81436
Western Blot: NDFIP2 Antibody [NBP1-81436] - Analysis in human cell line A-431.
Immunocytochemistry/Immunofluorescence: NDFIP2 Antibody [NBP1-81436] - Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
Immunohistochemistry-Paraffin: NDFIP2 Antibody [NBP1-81436] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Neuropeptide Y
Antibody
NBP1-46535
Neuropeptide Y Antibody
(8 Publications)
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, IHC, IHC-Fr, IHC-P
PPAR gamma/NR1C3
Antibody
NBP2-22106
Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P
HSF4 Antibody
(504524)
MAB5695
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, human LNCaP human prostate cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF4 Monoclonal Antibody (Catalog # MAB5695) followed by HRP-conjugated Anti‑Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSF4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Species: Hu, Mu
Applications: WB
LANCL2 Antibody
NBP1-83339
Western Blot: LANCL2 Antibody [NBP1-83339] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: LANCL2 Antibody [NBP1-83339] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: LANCL2 Antibody [NBP1-83339] - Staining of human hippocampus shows nuclear and cytoplasmic positivity in neuronal cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]
(4 Reviews)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - (5 ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - IHC analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. Image courtesy of anonymous customer product review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. Image from a confirmed customer review.
(3 Reviews)
(11 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
HtrA3 Antibody
NB600-1151
HtrA3 Antibody
Species: Hu, Mu, Rt, Fe
Applications: WB, ICC/IF
Supplier Logo

Publications for HtrA3 Antibody (NB600-1151)(1)

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
All Species

Reviews for HtrA3 Antibody (NB600-1151) (0)

There are no reviews for HtrA3 Antibody (NB600-1151). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HtrA3 Antibody (NB600-1151) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NB600-1151

Bioinformatics Tool for HtrA3 Antibody (NB600-1151)

Discover related pathways, diseases and genes to HtrA3 Antibody (NB600-1151). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HtrA3 Antibody (NB600-1151)

Discover more about diseases related to HtrA3 Antibody (NB600-1151).
 

Pathways for HtrA3 Antibody (NB600-1151)

View related products by pathway.

PTMs for HtrA3 Antibody (NB600-1151)

Learn more about PTMs related to HtrA3 Antibody (NB600-1151).

Blogs on HtrA3

There are no specific blogs for HtrA3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HtrA3 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol HTRA3
Entrez
Uniprot