HtrA3 Antibody - BSA Free

Western Blot: HtrA3 Antibody [NB600-1151] - Western bot analysis of HtrA3 in rat heart lysate using NB600-1151 at 2 ug/ml.

Western Blot: HtrA3 Antibody [NB600-1151] - analysis of HtrA1/2/3/4 in heart lysate from A) human, B) mouse and C) rat using this antibody.

• Reactivity: Hu, Mu, Rt, Fe
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

HtrA3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HtrA3 Antibody - BSA Free (NB600-1151) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-HtrA3 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Amino acids 273-287 (IVSTAQREGRELGLR) of human HtrA3 were used as immunogen.
• Localization: Secreted
• Specificity: The amino acid sequence is 100% homologous in HtrA3, 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4.
• Predicted Species: Feline (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTRA3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000. Use reported in scientific literature (Beleford et al)
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot: Detects a band of approximately 43 kDa (predicted molecular weight: 49 kDa).

Reactivity Notes

Predicted to react with Chicken and Feline

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for HtrA3 Antibody - BSA Free

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.108
• High-temperature requirement factor A3
• HTRA 3
• HtrA serine peptidase 3
• HTRA3
• Pregnancy-related serine protease
• probable serine protease HTRA3
• Prsp
• serine protease HTRA3
• Tasp

Background

HtrA3 belongs to the human high-temperature requirement factor A (HtrA) family. HtrA3 has two isoforms (long and short), which are produced by alternative splicing. Similar to HtrA1, HtrA3 binds to various TGF-beta proteins and inhibits the signaling of BMP-4, -2 and TGF-beta 1. HtrA3 does not inhibit signaling originated from a constitutively active BMP receptor, indicating that the inhibition occurs upstream of the cell surface receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HtrA3 Antibody (NB600-1151)(3)

Publications using NB600-1151

• Wu Y, Rong X, Pan M et al. Integrated Analysis Reveals the Gut Microbial Metabolite TMAO Promotes Inflammatory Hepatocellular Carcinoma by Upregulating POSTN Frontiers in Cell and Developmental Biology 2022-05-23 [PMID: 35676936] (Western Blot)
• Ko T, Nomura S, Yamada S et al. Cardiac fibroblasts regulate the development of heart failure via Htra3-TGF-beta-IGFBP7 axis Nature communications 2022-06-07 [PMID: 35672400] (WB, Mouse)
• Beleford D, Rattan R, Chien J, Shridhar V High temperature requirement A3 (HtrA3) promotes EPE- and cisplatin-induced cytotoxicity in lung cancer cell lines J Biol Chem 2010-04-16 [PMID: 20154083] (WB)

Bioinformatics

• Gene Symbol: HTRA3
• Entrez:
  • 94031(Human)
  • 78558(Mouse)
  • 360959(Rat)
• Uniprot:
  • P83110(Human)