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HtrA3 Antibody

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Western Blot: HtrA3 Antibody [NB600-1151] - Western bot analysis of HtrA3 in rat heart lysate using NB600-1151 at 2 ug/ml.
Western Blot: HtrA3 Antibody [NB600-1151] - Western bot analysis of HtrA3 in rat heart lysate using NB600-1151 at 2 ug/ml.
Western Blot: HtrA3 Antibody [NB600-1151] - analysis of HtrA1/2/3/4 in heart lysate from A) human, B) mouse and C) rat using this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, FeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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HtrA3 Antibody Summary

Immunogen
Amino acids 273-287 (IVSTAQREGRELGLR) of human HtrA3 were used as immunogen.
Localization
Secreted
Specificity
The amino acid sequence is 100% homologous in HtrA3, 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4.
Predicted Species
Feline (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HTRA3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Western Blot: Detects a band of approximately 43 kDa (predicted molecular weight: 49 kDa). Immunocytochemistry/Immunofluorescence: See Beleford et al for more details.
Publications
Read Publication using NB600-1151.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Predicted to react with Chicken and Cat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.2% Gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for HtrA3 Antibody

  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.108
  • High-temperature requirement factor A3
  • HtrA serine peptidase 3
  • Pregnancy-related serine protease
  • probable serine protease HTRA3
  • Prsp
  • serine protease HTRA3
  • Tasp

Background

HtrA3 belongs to the human high-temperature requirement factor A (HtrA) family. HtrA3 has two isoforms (long and short), which are produced by alternative splicing. Similar to HtrA1, HtrA3 binds to various TGF-beta proteins and inhibits the signaling of BMP-4, -2 and TGF-beta 1. HtrA3 does not inhibit signaling originated from a constitutively active BMP receptor, indicating that the inhibition occurs upstream of the cell surface receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Detection of Human and Mouse CRSP1(TRAP220) on HeLa whole cell lysate using NB100-2574.
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Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - IHC analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. Image courtesy of anonymous customer product review.
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Publications for HtrA3 Antibody (NB600-1151)(1)

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol HTRA3
Entrez
Uniprot