HTRA2/Omi Antibody (1B3) - BSA Free

Western Blot: HTRA2/Omi Antibody (1B3) [NBP1-04325] - The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HTRA2/Omi Antibody (1B3) - BSA Free Summary

• Description: Novus Biologicals Mouse HTRA2/Omi Antibody (1B3) - BSA Free (NBP1-04325) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human HtrA2/Omi (134-458 aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HTRA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HTRA2/Omi Antibody (1B3) - BSA Free

• High temperature requirement protein A2
• HtrA serine peptidase 2
• HTRA2
• HtrA-like serine protease
• Omi
• OMIOmi stress-regulated endoprotease
• PARK13EC 3.4.21.108
• PRSS25
• PRSS25serine, 25
• Serine protease 25
• serine protease HTRA2, mitochondrial
• Serine proteinase OMI

Background

HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HTRA2
• Entrez:
  • 297376(Rat)
• Uniprot:
  • NP_037379(Human)