HSPA8/HSC71/Hsc70 Antibody

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Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - A. 30 ug 293T whole cell lysate/extractB. 30 ug A431whole cell lysate/extractC. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract7.5 % ...read more
Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSC70 protein stained by HSC70 antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 ...read more
Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16882] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.25 mg/ml

Order Details

HSPA8/HSC71/Hsc70 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.
Localization
Cytoplasm; Melanosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-16882 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%), Xenopus laevis (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSPA8/HSC71/Hsc70 Antibody

  • Heat shock 70 kDa protein 8
  • heat shock 70kD protein 8
  • heat shock 70kDa protein 8
  • heat shock cognate 71 kDa protein
  • heat shock cognate protein 54
  • heat shock cognate protein, 71-kDa
  • HSC54
  • HSC70
  • HSC70heat shock 70kd protein 10
  • HSC71
  • HSP71
  • HSP73
  • HSP73constitutive heat shock protein 70
  • HSPA10
  • HSPA8
  • LAP1
  • lipopolysaccharide-associated protein 1
  • LPS-associated protein 1
  • MGC131511
  • MGC29929
  • NIP71
  • N-myristoyltransferase inhibitor protein 71

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSPA8/HSC71/Hsc70 Antibody (NBP2-16882) (0)

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Review for HSPA8/HSC71/Hsc70 Antibody (NBP2-16882) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Opsanus beta.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-16882:
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Western Blot HSPA8/HSC71/Hsc70 NBP2-16882
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reviewed by:
Kevin Schauer
WB Opsanus beta 02/15/2017
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Summary

ApplicationWestern Blot
Sample TestedAdult small intestine
SpeciesOpsanus beta
Lot40079

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Recent Reviews

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Kevin Schauer
02/15/2017
Application: WB
Species: Opsanus beta

Bioinformatics

Gene Symbol HSPA8
Entrez
Uniprot