HSPA8/HSC71/Hsc70 Antibody

Images

 
Western Blot: Hsc70 Antibody [NBP2-16881] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16881] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: Hsc70 Antibody [NBP2-16881] - Sample (20 ug of whole cell lysate) A: Raw264. 7 NBP2-16881 diluted at 1:2000.
Western Blot: Hsc70 Antibody [NBP2-16881] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.79 mg/ml

Order Details

HSPA8/HSC71/Hsc70 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.
Localization
Cytoplasm; Melanosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-16881 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.79 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSPA8/HSC71/Hsc70 Antibody

  • Heat shock 70 kDa protein 8
  • heat shock 70kD protein 8
  • heat shock 70kDa protein 8
  • heat shock cognate 71 kDa protein
  • heat shock cognate protein 54
  • heat shock cognate protein, 71-kDa
  • HSC54
  • HSC70
  • HSC70heat shock 70kd protein 10
  • HSC71
  • HSP71
  • HSP73
  • HSP73constitutive heat shock protein 70
  • HSPA10
  • HSPA8
  • LAP1
  • lipopolysaccharide-associated protein 1
  • LPS-associated protein 1
  • MGC131511
  • MGC29929
  • NIP71
  • N-myristoyltransferase inhibitor protein 71

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSPA8/HSC71/Hsc70 Antibody (NBP2-16881)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol HSPA8
Entrez
Uniprot