Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis using the Biotin conjugate of NBP1-97868. Detection of HSC70/HSP73, mAb (1B5) (biotin conjugate): Lane 1: HSC70/HSP73 (bovine), (rec); Lane 2: ...read more
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell ...read more
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73, mAb (1B5) (BIOTIN conjugate): Lane 1: HSC70/HSP73 (bovine), (rec); Lane 2: HSP70/HSP72 (human), (recombinant); Lane 3: 3T3 (heat ...read more
Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, ShSpecies Glossary
This antibody is useful in Electron Electron Microscopy, Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and detects a band of ~73kDa by Western blot.
Read 1 Review rated 4 using NBP1-97868 in the following applications:
Alternate Names for HSPA8/HSC71/Hsc70 Antibody (1B5)
Heat shock 70 kDa protein 8
heat shock 70kD protein 8
heat shock 70kDa protein 8
heat shock cognate 71 kDa protein
heat shock cognate protein 54
heat shock cognate protein, 71-kDa
HSC70heat shock 70kd protein 10
HSP73constitutive heat shock protein 70
lipopolysaccharide-associated protein 1
LPS-associated protein 1
N-myristoyltransferase inhibitor protein 71
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
NBP1-97868, on rat B103 cells (~125, 250, 750, and 1500 cell equivalents per lane [left to right]) using 1:25 dilution of antibody and Jackson Immuno HRP-conjugated anti-rabbit (1:100 dil). The signal is clean at ~70 kDa, although there is cross reactivity with the 230-kDa fluorescent migration control.
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