HSPA8/HSC71/Hsc70 Antibody (1B5)

Images

 
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis using the Biotin conjugate of NBP1-97868. Detection of HSC70/HSP73, mAb (1B5) (biotin conjugate): Lane 1: HSC70/HSP73 (bovine), (rec); Lane 2: ...read more
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell ...read more
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73, mAb (1B5) (BIOTIN conjugate): Lane 1: HSC70/HSP73 (bovine), (rec); Lane 2: HSP70/HSP72 (human), (recombinant); Lane 3: 3T3 (heat ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, ShSpecies Glossary
Applications WB, EIA, EM, Flow, ICC/IF, IHC, IHC-P, IP
Clone
1B5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

HSPA8/HSC71/Hsc70 Antibody (1B5) Summary

Immunogen
Native hamster Hsc70 (Hsp73).
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
HSPA8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Electron Microscopy 1:10-1:500
  • Enzyme Immunoassay
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody is useful in Electron Electron Microscopy, Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and detects a band of ~73kDa by Western blot.
Positive Control
HSPA8/HSC71/Hsc70 Lysate (NBP2-66130)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-97868 in the following applications:

Publications
Read Publications using NBP1-97868.

Reactivity Notes

Turtle

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HSPA8/HSC71/Hsc70 Antibody (1B5)

  • Heat shock 70 kDa protein 8
  • heat shock 70kD protein 8
  • heat shock 70kDa protein 8
  • heat shock cognate 71 kDa protein
  • heat shock cognate protein 54
  • heat shock cognate protein, 71-kDa
  • HSC54
  • HSC70
  • HSC70heat shock 70kd protein 10
  • HSC71
  • HSP71
  • HSP73
  • HSP73constitutive heat shock protein 70
  • HSPA10
  • HSPA8
  • LAP1
  • lipopolysaccharide-associated protein 1
  • LPS-associated protein 1
  • MGC131511
  • MGC29929
  • NIP71
  • N-myristoyltransferase inhibitor protein 71

Background

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-97868
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, Sh
Applications: WB, EIA, EM, Flow, ICC/IF, IHC, IHC-P, IP

Publications for HSPA8/HSC71/Hsc70 Antibody (NBP1-97868)(10)


Showing Publications 1 - 10 of 10.
Publications using NBP1-97868 Applications Species
Li PP, Itoh N, Watanabe M et al. Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens. J Virol. 2009 Jan. [PMID: 18945763]
Lu HA, Sun TX, Matsuzaki T et al. Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking. J Biol Chem. 2007 Sep. [PMID: 17636261]
Chen S, Brown IR. Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases. Cell Stress Chaperones. 2007 Spring [PMID: 17441507]
Tetzlaff J, Tanzer L, Jones KJ et al. Exogenous androgen treatment delays the stress response following hamster facial nerve injury. J Neuroendocrinol. 2007 May [PMID: 17425613]
Meinander A, Soderstrom TS, Kaunisto A et al. Fever-like hyperthermia controls T Lymphocyte persistence by inducing degradation of cellular FLIPshort. J Immunol. 2007 Mar. [PMID: 17339495]
Suttitanamongkol S, Polanowska-Grabowska R, Gear AR et al. Heat-shock protein 90 complexes in resting and thrombin-activated platelets. Biochem Biophys Res Commun. 2002 Sep. [PMID: 12220519]
Yamano T, Murata S, Shimbara N et al. Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing. J Exp Med. 2002 Jul. [PMID: 12119343]
Bailey CK, Andriola IF, Kampinga HH et al. Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy. Hum Mol Genet. 2002 Mar. [PMID: 11875046]
Brighty DW, Jassal SR. The synthetic peptide P-197 inhibits human T-cell leukemia virus type 1 envelope-mediated syncytium formation by a mechanism that is independent of Hsc70. J Virol. 2001 Nov. [PMID: 11581416]
Sainis L, Angelidis C, Pagoulatos GN et al. HSC70 interactions with SV40 viral proteins differ between permissive and nonpermissive mammalian cells. Cell Stress Chaperones. 2000 Apr. [PMID: 11147964]

Review for HSPA8/HSC71/Hsc70 Antibody (NBP1-97868) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-97868:
Filter by Applications
Simple Western
(1)
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(1)
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Simple Western HSPA8/HSC71/Hsc70 NBP1-97868
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reviewed by:
Anonymous
Simple Western Rat 01/05/2017
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Summary

ApplicationSimple Western
Sample TestedB103 Cells
SpeciesRat

Comments

CommentsNBP1-97868, on rat B103 cells (~125, 250, 750, and 1500 cell equivalents per lane [left to right]) using 1:25 dilution of antibody and Jackson Immuno HRP-conjugated anti-rabbit (1:100 dil). The signal is clean at ~70 kDa, although there is cross reactivity with the 230-kDa fluorescent migration control.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSPA8/HSC71/Hsc70 Antibody (NBP1-97868) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-97876

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Recent Reviews

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Anonymous
01/05/2017
Application: Simple Western
Species: Rat

Bioinformatics

Gene Symbol HSPA8
Entrez