Recombinant Human HspA6 His Protein

SDS-Page: Recombinant Human HspA6 Protein [NBP1-30291] - HSP70B, 73.2 kDa (663aa) with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: MA, PAGE
• Concentration: 1 mg/ml

Recombinant Human HspA6 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-643 of Human HspA6
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQAPRELAVG IDLGTTYSCV GVFQQGRVEI LANDQGNRTT PSYVAFTDTE RLVGDAAKSQ AALNPHNTVF DAKRLIGRKF ADTTVQSDMK HWPFRVVSEG GKPKVRVCYR GEDKTFYPEE ISSMVLSKMK ETAEAYLGQP VKHAVITVPA YFNDSQRQAT KDAGAIAGLN VLRIINEPTA AAIAYGLDRR GAGERNVLIF DLGGGTFDVS VLSIDAGVFE VKATAGDTHL GGEDFDNRLV NHFMEEFRRK HGKDLSGNKR ALRRLRTACE RAKRTLSSST QATLEIDSLF EGVDFYTSIT RARFEELCSD LFRSTLEPVE KALRDAKLDK AQIHDVVLVG GSTRIPKVQK LLQDFFNGKE LNKSINPDEA VAYGAAVQAA VLMGDKCEKV QDLLLLDVAP LSLGLETAGG VMTTLIQRNA TIPTKQTQTF TTYSDNQPGV FIQVYEGERA MTKDNNLLGR FELSGIPPAP RGVPQIEVTF DIDANGILSV TATDRSTGKA NKITITNDKG RLSKEEVERM VHEAEQYKAE DEAQRDRVAA KNSLEAHVFH VKGSLQEESL RDKIPEEDRR KMQDKCREVL AWLEHNQLAE KEEYEHQKRE LEQICRPIFS RLYGGPGVPG GSSCGTQARQ GDPSTGPIIE EVD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HSPA6
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Microarray
  • SDS-Page
• Application Notes: Use in Microarray reported in scientific literature (PMID:35059422)
• Theoretical MW: 73.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HspA6 His Protein

• heat shock 70 kDa protein 6
• Heat shock 70 kDa protein B'
• heat shock 70kD protein 6 (HSP70B')
• heat shock 70kDa protein 6 (HSP70B')
• HSP70B'

Background

HspA6 is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HspA6 and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HspA6, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for HspA6 Recombinant Protein (NBP1-30291)(1)

Publication using NBP1-30291

• Qi S, Li J, He X et al. Identification and Validation of Novel Serum Autoantibodies Biomarkers for Staging Liver Fibrosis in Patients With Chronic Hepatitis B Frontiers in medicine 2022-01-04 [PMID: 35059422] (MiAr)

Bioinformatics

• Gene Symbol: HSPA6